Isoflavone-beta-D-glucan produced by Agaricus blazei in the submerged liquid culture and method of producing same

Abstract

The present invention relates to low and medium molecular weight isoflavone-β-D-glucan produced by submerged liquid culture of Agaricus blazei, a method of producing the isoflavone-β-D-glucan using autolysis enzyme of Agaricus blazei mycelia, and use of the isoflavone-β-D-glucan for anti-cancer and immunoenhancing effect.

1 . A method of producing isoflavone-β-D-glucan, which comprises the steps of: 
 culturing  Agaricus blazei  mycelia in a liquid medium containing isoflavone to produce a high molecular weight isoflavone-β-D-glucan;    activating an autolysis enzyme in the liquid culture of  Agaricus blazei  mycelia to produce a low and medium molecular weight isoflavone-β-D-glucan; and    separating and purifying the low and medium molecular weight isoflavone-β-D-glucan.    
   
   
       2 . The method according to  claim 1 , wherein the step of activating an autolysis enzyme from the liquid culture of  Agaricus blazei  mycelia is carried out by adjusting the pH of culture medium to pH 4.5-5.5 for 1-3 hours.  
   
   
       3 . The isoflavone-β-D-glucan produced by the method of  claim 1 .  
   
   
       4 . An immunoenhancing agent comprising the isoflavone-β-D-glucan of  claim 3.