US2006068379A1PendingUtilityA1

Phage display of intact domains at high copy number

39
Individually held — no corporate assignee on recordPriority: Apr 11, 1997Filed: Jun 24, 2005Published: Mar 30, 2006
Est. expiryApr 11, 2017(expired)· nominal 20-yr term from priority
Inventors:Alasdair C. StevenPaul T. WingfieldLindsay W. BlackZhaojun Ren
C40B 40/02C12N 15/1037
39
PatentIndex Score
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Cited by
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References
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Claims

Abstract

Disclosed is a phage display system in which the molecules to be displayed (i.e., molecules of interest) are bound to dispensable capsid polypeptides such as SOC (small outer capsid) and HOC (highly antigenic outer capsid) polypeptides that are, in turn, bound to a surface lattice protein, such as those on the surface of a virion or polyhead. Also disclosed are methods of displaying a molecule of interest, methods of immunizing a patient by administering a displayed antigen, and methods of treating a patient who has a disorder associated with aberrent expression or activity of a biological molecule. In the latter instance, the method includes administering a displayed polypeptide, such as an immunoglobulin molecule or an enzyme, that is capable of specifically interacting with the aberrent biological molecule.

Claims

exact text as granted — not AI-modified
1 - 56 . (canceled)  
     
     
         57 . A method of making a system for displaying a molecule of interest, comprising contacting a T4 surface lattice protein array with a filamentous T4 phage-display system comprising a small outer capsid (SOC) fusion protein or highly antigenic outer capsid (HOC) fusion protein, wherein said fusion protein comprises the molecule of interest fused to the SOC or HOC protein, wherein said SOC or HOC fusion protein is capable of binding to a T4 surface protein.  
     
     
         58 . The method of  claim 57 , wherein the molecule of interest is an antigen.  
     
     
         59 . The method of  claim 57  wherein the molecule of interest is an enzyme.  
     
     
         60 . The method of  claim 57 , wherein the molecule of interest is an immunoglobulin.  
     
     
         61 . The method of  claim 57 , wherein the molecule of interest is a polypeptide.  
     
     
         62 . The method of  claim 61 , wherein the polypeptide consists of 4 or more amino acids.  
     
     
         63 . The method of  claim 61 , wherein the polypeptide consists of 40 or more amino acids.  
     
     
         64 . The method of  claim 61 , wherein the polypeptide consists of 300 or more amino acids.  
     
     
         65 . The method of  claim 57 , wherein at least 100 copies of the molecule of interest are displayed on the T4 surface lattice protein array.  
     
     
         66 . The method of  claim 57 , wherein at least 1000 copies of the molecule of interest are displayed on the T4 surface lattice protein array.  
     
     
         67 . The method of  claim 57 , wherein at least 10000 copies of the molecule of interest are displayed on the T4 surface lattice protein array.  
     
     
         68 . The method of  claim 57 , wherein the molecule of interest is bound to a carboxyl-terminus of the SOC or HOC protein.  
     
     
         69 . The method of  claim 57 , wherein the molecule of interest is bound to an amino-terminus of the SOC or HOC protein.  
     
     
         70 . A method of making a system for displaying a molecule of interest on a T4 surface lattice protein array, comprising: integrating into a virion's genome from which all or part of a nucleic acid encoding a wild type small outer capsid (SOC) protein or highly antigenic outer capsid (HOC) protein has been deleted, a nucleic acid molecule encoding a SOC or HOC fusion protein, wherein said fusion protein comprises the molecule of interest fused to the SOC or HOC protein, wherein said SOC or HOC fusion protein is capable of binding to a T4 surface protein; and wherein the molecule of interest is displayed on a T4 surface lattice protein array.  
     
     
         71 . The method of  claim 70 , wherein the molecule of interest is an antigen.  
     
     
         72 . The method of  claim 70 , wherein the molecule of interest is an enzyme.  
     
     
         73 . The method of  claim 70 , wherein the molecule of interest is an immunoglobulin.  
     
     
         74 . The method of  claim 70 , wherein the molecule of interest is a polypeptide.  
     
     
         75 . The method of  claim 74 , wherein the polypeptide consists of 4 or more amino acids.  
     
     
         76 . The method of  claim 74 , wherein the polypeptide consists of 40 or more amino acids.  
     
     
         77 . The method of  claim 74 , wherein the polypeptide consists of 300 or more amino acids.  
     
     
         78 . The method of  claim 70 , wherein at least 100 copies of the molecule of interest are displayed on the T4 surface lattice protein array.  
     
     
         79 . The method of  claim 70 , wherein at least 1000 copies of the molecule of interest are displayed on the T4 surface lattice protein array.  
     
     
         80 . The method of  claim 70 , wherein at least 10000 copies of the molecule of interest are displayed on the T4 surface lattice protein array.  
     
     
         81 . The method of  claim 70 , wherein the molecule of interest is bound to a carboxyl-terminus of the SOC or HOC protein.  
     
     
         82 . The method of  claim 70 , wherein the molecule of interest is bound to an amino-terminus of the SOC or HOC protein.

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