US2006060211A1PendingUtilityA1

Modifying nicotine and nitrosamine levels in tobacco

59
Individually held — no corporate assignee on recordPriority: Jun 8, 2001Filed: Nov 22, 2005Published: Mar 23, 2006
Est. expiryJun 8, 2021(expired)· nominal 20-yr term from priority
Inventors:Mark Conkling
A61P 35/00A61P 25/34A24B 15/20A24B 15/18A24B 15/243C12N 9/1077C12N 15/8243C07K 14/415A24B 15/245A24B 15/10
59
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Claims

Abstract

The present invention generally relates to tobacco and tobacco products having a reduced amount of nicotine and/or tobacco specific nitrosamines (TSNA). More specifically, several ways to make tobacco plants that have reduced nicotine and TSNA levels have been discovered. Embodiments include tobacco harvested from said tobacco plants, cured tobacco from said tobacco plants, tobacco products made with said cured tobacco and methods of making these compositions.

Claims

exact text as granted — not AI-modified
1 . A tobacco product comprising a cured tobacco that comprises a genetic modification, wherein said tobacco product has a collective content of N′-nitrosonomicotine (NNN), N′-nitrosoanatabine (NAT), N′-nitrosoanabasine (NAB), 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) of less than 0.5 μg/g.  
     
     
         2 . The tobacco product of  claim 1 , wherein said cured tobacco is of the genus  Nicotiana.    
     
     
         3 . The tobacco product of  claim 2 , wherein said cured tobacco is selected from the group consisting of Burley, Flue, and Oriental.  
     
     
         4 . The tobacco product of  claim 2 , wherein said cured tobacco is Burley.  
     
     
         5 . The tobacco product of  claim 2 , wherein said cured tobacco is Flue.  
     
     
         6 . The tobacco product of  claim 1 , wherein said cured tobacco comprises an exogenous nucleic acid that encodes an enzyme in the nicotine synthesis pathway or a fragment thereof at least 25 nucleotides in length.  
     
     
         7 . The tobacco product of  claim 1 , wherein said cured tobacco comprises a fragment of a nucleic acid that encodes an enzyme in the nicotine synthesis pathway that is at least 50 nucleotides in length.  
     
     
         8 . The tobacco product of  claim 1 , wherein said cured tobacco comprises a fragment of a nucleic acid that encodes an enzyme in the nicotine synthesis pathway that is at least 100 nucleotides in length.  
     
     
         9 . The tobacco product of  claim 1 , wherein said cured tobacco comprises a fragment of a nucleic acid that encodes an enzyme in the nicotine synthesis pathway that is at least 250 nucleotides in length.  
     
     
         10 . The tobacco product of  claim 1 , wherein the amount of nicotine in said product is less than 11 mg/g.  
     
     
         11 . The tobacco product of  claim 1 , wherein the amount of nicotine in said product is less than 9 mg/g.  
     
     
         12 . The tobacco product of  claim 1 , wherein the amount of nicotine in said product is less than 7 mg/g.  
     
     
         13 . The tobacco product of  claim 1 , wherein the amount of nicotine in said product is less than 5 mg/g.  
     
     
         14 . The tobacco product of  claim 1 , wherein the amount of nicotine in said product is less than 3 mg/g.  
     
     
         15 . The tobacco product of  claim 1 , wherein the amount of nicotine in said product is less than 2 mg/g.  
     
     
         16 . The tobacco product of  claim 1 , wherein the amount of nicotine in said product is less than 1 mg/g.  
     
     
         17 . The tobacco product of  claim 1 , wherein the amount of nicotine in said product is less than 0.7 mg/g.  
     
     
         18 . The tobacco product of  claim 1 , wherein the amount of nicotine in said product is less than 0.5 mg/g.  
     
     
         19 . The tobacco product of  claim 1 , wherein the amount of nicotine in said product is less than 0.1 mg/g.  
     
     
         20 . The tobacco product of  claim 1 , wherein the collective content of N′-nitrosonomicotine (NNN), N′-nitrosoanatabine (NAT), N′-nitrosoanabasine (NAB), 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) is less than 0.4 μg/g.  
     
     
         21 . The tobacco product of  claim 1 , wherein the collective content of N′-nitrosonomicotine (NNN), N′-nitrosoanatabine (NAT), N′-nitrosoanabasine (NAB), 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) is less than 0.3 μg/g.  
     
     
         22 . The tobacco product of  claim 1 , wherein the collective content of N′-nitrosonornicotine (NNN), N′-nitrosoanatabine (NAT), N′-nitrosoanabasine (NAB), 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) is less than 0.2 μg/g.  
     
     
         23 . The tobacco product of  claim 1 , wherein the collective content of N′-nitrosonornicotine (NNN), N′-nitrosoanatabine (NAT), N′-nitrosoanabasine (NAB), 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) is less than 0.1 μg/g.  
     
     
         24 . The tobacco product of  claim 1 , wherein the collective content of N′-nitrosonornicotine (NNN), N′-nitrosoanatabine (NAT), N′-nitrosoanabasine (NAB), 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) is less than 0.05 μg/g.  
     
     
         25 . The tobacco product of  claim 1 , wherein the collective content of N′-nitrosonornicotine (NNN), N′-nitrosoanatabine (NAT), N′-nitrosoanabasine (NAB), 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) is less than 0.02 μg/g.  
     
     
         26 . The tobacco product of  claim 1 , wherein the collective content of N′-nitrosonornicotine (NNN), N′-nitrosoanatabine (NAT), N′-nitrosoanabasine (NAB), 4-(N-nitrosomethylamino)-1-(3-pyridyl)-1-butanone (NNK) is less than 0.01 μg/g.  
     
     
         27 . The tobacco product of  claim 6 , wherein said enzyme in the nicotine synthesis pathway is selected from the group consisting of putrescine N-methyltransferase, N-methylputrescine oxidase, ornithine decarboxylase, S-adenosylmethionine synthetase, NADH dehydrogenase, phosphoribosylanthranilate isomerase, and quinolate phosphoribosyl transferase.  
     
     
         28 . The tobacco product of  claim 7 , wherein said enzyme in the nicotine synthesis pathway is selected from the group consisting of putrescine N-methyltransferase, N-methylputrescine oxidase, ornithine decarboxylase, S-adenosylmethionine synthetase, NADH dehydrogenase, phosphoribosylanthranilate isomerase, and quinolate phosphoribosyl transferase.  
     
     
         29 . The tobacco product of  claim 8 , wherein said enzyme in the nicotine synthesis pathway is selected from the group consisting of putrescine N-methyltransferase, N-methylputrescine oxidase, ornithine decarboxylase, S-adenosylmethionine synthetase, NADH dehydrogenase, phosphoribosylanthranilate isomerase, and quinolate phosphoribosyl transferase.  
     
     
         30 . The tobacco product of  claim 9 , wherein said enzyme in the nicotine synthesis pathway is selected from the group consisting of putrescine N-methyltransferase, N-methylputrescine oxidase, ornithine decarboxylase, S-adenosylmethionine synthetase, NADH dehydrogenase, phosphoribosylanthranilate isomerase, and quinolate phosphoribosyl transferase.  
     
     
         31 . The tobacco product of  claim 1 , wherein said tobacco product is a cigarette.  
     
     
         32 . The tobacco product of  claim 6 , wherein said tobacco product is a cigarette.  
     
     
         33 . The tobacco product of  claim 10 , wherein said tobacco product is a cigarette.  
     
     
         34 . The tobacco product of  claim 15 , wherein said tobacco product is a cigarette.  
     
     
         35 . The tobacco product of  claim 18 , wherein said tobacco product is a cigarette.  
     
     
         36 . The tobacco product of  claim 22 , wherein said tobacco product is a cigarette.  
     
     
         37 . The tobacco product of  claim 25 , wherein said tobacco product is a cigarette.

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