Biallelic markers related to genes involved in drug metabolism

Abstract

The invention provides polynucleotides including biallelic markers derived from genes involved in the biotransformation of xenobiotics such as drugs and from genomic regions flanking those genes. Primers hybridizing to regions flanking these biallelic markers are also provided. This invention also provides polynucleotides and methods suitable for genotyping a nucleic acid containing sample for one or more biallelic markers of the invention. Further, the invention provides methods to detect a statistical correlation between a biallelic marker allele and a phenotype and/or between a biallelic marker haplotype and a phenotype.

1 . A method of determining the genotype of an individual comprising the steps of: 
 a) obtaining a biological sample containing a polynucleotide comprising the nucleotide sequence of SEQ ID NO: 13 from said individual; and    b) determining the identity of a nucleotide at a biallelic marker of SEQ ID NO: 13 wherein said nucleotide determines said genotype of said individual.    
     
     
         2 . The method of  claim 1 , wherein said nucleotide is located at position 501 in SEQ ID NO: 13.  
     
     
         3 . The method of  claim 1 , further comprising amplifying a portion of said polynucleotide comprising said biallelic marker prior to said determining step.  
     
     
         4 . The method of  claim 2 , further comprising amplifying a portion of said polynucleotide comprising said biallelic marker prior to said determining step.  
     
     
         5 . The method of  claim 3 , wherein said amplifying is performed by PCR.  
     
     
         6 . The method of  claim 4 , wherein said amplifying is performed by PCR.  
     
     
         7 . The method of  claim 1 , wherein said determining is performed by an assay selected from the group consisting of hybridization assay, sequencing assay, microsequencing assay, and enzyme-based mismatch detection assay.  
     
     
         8 . An isolated polynucleotide comprising SEQ ID NO: 13.