US2006035323A2PendingUtilityA2
Method for producing sweetners and alcohol
Est. expiryFeb 3, 2015(expired)· nominal 20-yr term from priority
D06L 1/14C11D 3/38681C11D 3/38618C12N 9/2417C12Y 302/01001C11D 3/38609
54
PatentIndex Score
0
Cited by
0
References
0
Claims
Abstract
Abstract of the Disclosure The present invention relates to variants of a parent α-amylase, which parent α-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an α-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an α-amylase having one of these amino acid sequences; in which variant:(a) at least one amino acid residue of the parent α-amylase has been deleted; and/or(b) at least one amino acid residue of the parent α-amylase has been replaced by a different amino acid residue; and/or(c) at least one amino acid residue has been inserted relative to the parent α-amylase;the variant having α-amylase activity and exhibiting at least one of the following properties relative to the parent α-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca 2+ dependency;with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.
Claims
exact text as granted — not AI-modified1. Cancelled.
2. Cancelled.
3. Cancelled.
4. Cancelled.
5. Cancelled.
6. Cancelled.
7. Cancelled.
8. Cancelled.
9. Cancelled.
10. Cancelled.
11. Cancelled.
12. Cancelled.
13. Cancelled.\
14. Cancelled.
15. Cancelled.
16. Cancelled.
17. Cancelled.
18. Cancelled.
19. Cancelled.
20. Cancelled.
21. Cancelled.
22. Cancelled.
23. Cancelled.
24. Cancelled.
25. Cancelled.
26. Cancelled.
27. Cancelled.
28. Cancelled.
29. Cancelled.
30. Cancelled.
31. Cancelled.
32. Cancelled.
33. Cancelled.
34. Cancelled.
35. Cancelled.
36. Cancelled.
37. Cancelled.
38. Cancelled.
39. Cancelled.
40. Cancelled.
41. Cancelled.
42. Cancelled.
43. Cancelled.
44. Cancelled.
45. Cancelled.
46. Cancelled.
47. Cancelled.
48. Cancelled.
49. Cancelled.
50. Cancelled.
51. Cancelled.
52. Cancelled.
53. Cancelled.
54. Cancelled.
55. Cancelled.
56. Cancelled.
57. Cancelled.
58. Cancelled.
59. Cancelled.
60. Cancelled.
61. Cancelled.
62. Cancelled.
63. Cancelled.
64. Cancelled.
65. Cancelled.
66. Cancelled.
67. Cancelled.
68. Cancelled.
69. Cancelled.
70. Cancelled.
71. Cancelled.
72. Cancelled.
73. Cancelled.
74. Cancelled.
75. Cancelled.
76. Cancelled.
77. Cancelled.
78. Cancelled.
79. Cancelled.
80. Cancelled.
81. Cancelled.
82. Cancelled.
83. Cancelled.
84. Cancelled.
85. Cancelled.
86. Cancelled.
87. Cancelled.
88. Cancelled.
89. Cancelled.
90. Cancelled.
91. Cancelled.
92. Cancelled.
93. Cancelled.
94. Cancelled.
95. Cancelled.
96. Cancelled.
97. Cancelled.
98. Cancelled.
99. Cancelled.
100. Cancelled.
101. Cancelled.
102. Cancelled.
103. Cancelled.
104. Cancelled.
105. Cancelled.
106. Cancelled.
107. Cancelled.
108. Cancelled.
109. Cancelled.
110. Cancelled.
111. Cancelled.
112. Cancelled.
113. Cancelled.
114. Cancelled.
115. Cancelled.
116. Cancelled.
117. Cancelled.
118. A nucleic acid encoding a variant of a parent Bacillus stearothermophilus alpha-amylase, wherein the variant has an amino acid sequence which has at least 95% homology to the parent Bacillus stearothermophilus alpha-amylase and comprises a deletion of amino acids 179 and 180, using SEQ ID NO:3 for numbering, and wherein the variant has alpha-amylase activity
119. The nucleic acid of claim 118 , wherein the variant further comprises a substitution of a cysteine at amino acids 349 and 428, using SEQ ID NO:3 for numbering.
120. A nucleic acid construct comprising the nucleic acid of claim 118 operably linked to one or more control sequences that direct the production of the variant in a suitable expression host.
121. The nucleic acid construct of claim 120 , wherein one or more control sequence directs the production of the variant in a bacterial host.
122. The nucleic acid construct of claim 120 , wherein one or more control sequence directs the production of the variant in a fungal host.
123. A recombinant expression vector comprising the nucleic acid construct of claim 120 .
124. A recombinant host cell comprising the nucleic acid construct of claim 120 .
125. The recombinant host cell of claim 124 , wherein the host cell is a bacterial cell.
126. The recombinant host cell of claim 124 , wherein the host cell is a fungal cell.
127. The recombinant host cell of claim 124 , wherein the host cell is a yeast cell.
128. The recombinant host cell of claim 124 , wherein the host cell is a species of Bacillus .
129. The recombinant host cell of claim 124 , wherein the host cell is selected from the group consisting of Bacillus subtilis, Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus circulans, Bacillus lautus, Bacillus megaterium, Bacillus thuringiensis, Streptomyces lividans and Streptomyces murinus.
130. The recombinant host cell of claim 124 , wherein the host cell is Bacillus licheniformis.
131. A method for producing a variant alpha-amylase, which method comprises: (a) cultivating a host cell comprising the nucleic acid of claim 118 and (b) recovering the variant alpha-amylase from the host cell.
132. The method of claim 131 , wherein the host cell is a bacterial cell.
133. The method of claim 131 , wherein the host cell is a fungal cell.
134. The method of claim 131 , wherein the host cell is a yeast cell.
135. The method of claim 131 , wherein the host cell is a species of Bacillus .
136. The method of claim 131 , wherein the host cell is selected from the group consisting of Bacillus subtilis, Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus circulans, Bacillus lautus, Bacillus megaterium, Bacillus thuringiensis, Streptomyces lividans and Streptomyces murinus.
137. The method of claim 131 , wherein the host cell is Bacillus licheniformis.
138. The method of claim 131 , wherein the variant alpha-amylase is secreted from the host cell.
139. A nucleic acid encoding a variant alpha-amylase, wherein the variant has at least 95% homology to SEQ ID NO:3 and comprises a deletion of amino acids 179 and 180, using SEQ ID NO:3 for numbering, and wherein the variant has alpha-amylase activity .
140. A nucleic acid of claim 139 , wherein the variant further comprises a substitution of a cysteine at amino acids 349 and 428, using SEQ ID NO:3 for numbering.
141. A nucleic acid construct comprising the nucleic acid of claim 139 operably linked to one or more control sequences that direct the production of the variant in a suitable expression host.
142. A nucleic acid construct of claim 141 , wherein one or more control sequence directs the production of the variant in a bacterial host.
143. A nucleic acid construct of claim 141 , wherein one or more control sequence directs the production of the variant in a fungal host
144. A recombinant expression vector comprising the nucleic acid of claim 139 .
145. A recombinant host cell comprising the nucleic acid construct of claim 141 .
146. The recombinant host cell of claim 145 , wherein the host cell is a bacterial cell.
147. The recombinant host cell of claim 145 , wherein the host cell is a fungal cell.
148. The recombinant host cell of claim 145 , wherein the host cell is a yeast cell.
149. The recombinant host cell of claim 145 , wherein the host cell is a species of Bacillus.
150. The recombinant host cell of claim 145 , wherein the host cell is selected from the group consisting of Bacillus subtilis, Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus circulans, Bacillus lautus, Bacillus megaterium, Bacillus thuringiensis, Streptomyces lividans and Streptomyces murinus.
151. The recombinant host cell of claim 145 , wherein the host cell is Bacillus licheniformis.
152. A method for expressing a variant alpha-amylase, which method comprises: (a) cultivating a host cell comprising the nucleic acid of claim 139 and (b) recovering the variant alpha-amylase from the host cell.
153. The method of claim 152 , wherein the host cell is a bacterial cell.
154. The method of claim 152 , wherein the host cell is a fungal cell.
155. The method of claim 152 , wherein the host cell is a yeast cell.
156. The method of claim 152 , wherein the host cell is a species of Bacillus .
157. The method of claim 152 , wherein the host cell is selected from the group consisting of Bacillus subtilis, Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus circulans, Bacillus lautus, Bacillus megaterium, Bacillus thuringiensis, Streptomyces lividans and Streptomyces murinus.
158. The method of claim 152 , wherein the host cell is Bacillus licheniformis.
159. The method of claim 152 , wherein the variant alpha-amylase is secreted from the host cell.
160. A nucleic acid sequence encoding a variant of a Bacillus stearothermophilus alpha-amylase, wherein the alpha-amylase variant consists of a deletion of amino acids 179 and 180, using SEQ ID NO:3 for numbering.
161. A nucleic construct comprising the nucleic sequence of claim 160 operably linked to one or more control sequence that direct the production of the variant in a suitable expression host.
162. The nucleic acid construct of claim 161 , wherein one or more control sequences directs the production of the variant in a bacterial host.
163. The nucleic acid construct of claim 161 , wherein one or more control sequence directs the production of the variant in a fungal host.
164. A recombinant expression vector comprising the nucleic acid construct of claim 161 .
165. A recombinant host cell comprising the nucleic construct of claim 161 .
166. The recombinant host cell of claim 165 , wherein the host cell is a bacterial cell.
167. The recombinant host cell of claim 165 , wherein the host cell is a fungal cell.
168. The recombinant host cell of claim 165 , wherein the host cell is a yeast cell.
169. The recombinant host cell of claim 165 , wherein the host cell is a species of Bacillus.
170. The recombinant host cell of claim 165 , wherein the host cell is selected from the group consisting of Bacillus subtilis, Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus circulans, Bacillus lautus, Bacillus megaterium, Bacillus thuringiensis, Streptomyces lividans and Streptomyces murinus.
171. The recombinant host cell of claim 165 , wherein the host cell is Bacillus licheniformis.
172. A method for producing a variant alpha-amylase, which method comprises:
(a) cultivating a host cell comprising the nucleic acid of claim 160 and (b) recovering the variant alpha-amylase from the host cell.
173. The method of claim 172 , wherein the host cell is a bacterial cell.
174. The method of claim 172 , wherein the host cell is a fungal cell.
175. The method of claim 172 , wherein the host cell is a yeast cell.
176. The method of claim 172 , wherein the host cell is a species of Bacillus .
177. The method of claim 172 , wherein the host cell is selected from the group consisting of Bacillus subtilis, Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus circulans, Bacillus lautus, Bacillus megaterium, Bacillus thuringiensis, Streptomyces lividans and Streptomyces murinus.
178. The method of claim 172 , wherein the host cell is Bacillus licheniformis.
179. The method of claim 172 , wherein the variant alpha-amylase is secreted from the host cell.Join the waitlist — get patent alerts
Track US2006035323A2 — get alerts on status changes and closely related new filings.
We store only your email — no account needed. See our privacy policy.