Adipocyte complement related protein homolog zacrp3

Abstract

The present invention relates to polynucleotide and polypeptide molecules for zacrp3, a novel member of the family of proteins bearing a collagen-like domain and a C1q domain. The polypeptides and polynucleotides encoding them, are involved in dimerization or oligomerization and may be used in the study thereof. The present invention also includes antibodies to the zacrp3 polypeptides.

1 . An isolated polynucleotide encoding a polypeptide comprising amino acid residues 51-246 of SEQ ID NO:2.  
     
     
         2 . The isolated polynucleotide of  claim 1  wherein the polypeptide comprises amino acid residues 23-246 SEQ ID NO:2.  
     
     
         3 . The isolated polynucleotide of  claim 1  wherein the polypeptide comprises Gly-Xaa-Xaa repeats and Gly-Xaa-Pro repeats forming a collagen domain.  
     
     
         4 . The isolated polynucleotide of  claim 3  wherein the collagen domain consists of 15 Gly-Xaa-Xaa repeats and 6 Gly-Xaa-Pro repeats.  
     
     
         5 . The isolated polynucleotide of  claim 3  wherein the collagen domain comprises amino acid residues 51-113 of SEQ ID NO:2.  
     
     
         6 . The isolated polynucleotide of  claim 1  wherein the polypeptide comprises a carboxyl-terminal C1q domain which consists of 10 beta strands.  
     
     
         7 . The isolated polynucleotide of  claim 6  wherein the carboxyl-terminal C1q domain comprises an amino acid sequence of SEQ ID NO:5.  
     
     
         8 . The isolated polynucleotide of  claim 6  wherein the carboxy-terminal C1q domain comprises amino acid residues 119-123, 140-142, 148-151, 155-158, 161-173, 175-182, 190-197, 200-212, 217-222 and 236-241 of SEQ ID NO:2.  
     
     
         9 . The isolated polynucleotide of  claim 6  wherein the carboxy-terminal C1q domain consists of amino acid residues 114-246 of SEQ ID NO:2.  
     
     
         10 . The isolated polynucleotide of  claim 1  wherein the polypeptide specifically binds with an antibody that specifically binds with a polypeptide consisting of the amino acid sequence of SEQ ID NO:2.  
     
     
         11 . An isolated polynucleotide encoding a polypeptide comprising amino acid residues 23-246 of SEQ ID NO:2.  
     
     
         12 . An isolated polynucleotide encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:2.  
     
     
         13 . An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: 
 a) nucleotide 1 to nucleotide 1696 of SEQ ID NO:1;    b) nucleotide 69 to nucleotide 806 of SEQ ID NO:1;    c) nucleotide 135 to nucleotide 806 of SEQ ID NO:1;    d) nucleotide 219 to nucleotide 806 of SEQ ID NO:1;    e) nucleotide 408 to nucleotide 806 of SEQ ID NO:1;    f) nucleotide 69 to nucleotide 407 of SEQ ID NO:1;    g) nucleotide 135 to nucleotide 407 of SEQ ID NO:1; and    h) nucleotide 219 to nucleotide 407 of SEQ ID NO:1.    
     
     
         14 . An isolated polynucleotide encoding a fusion protein comprising a first portion and a second portion joined by a peptide bond, wherein the first portion is selected from the group consisting of: 
 a) a polypeptide comprising amino acid residues 51 to 246 of SEQ ID NO:2;    b) a polypeptide comprising amino acid residues 1 to 246 of SEQ ID NO:2; and    c) a polypeptide comprising amino acid residues 23 to 246 of SEQ ID NO:2; and    wherein the second portion comprises another polypeptide.    
     
     
         15 . An isolated polynucleotide consisting of a nucleotide sequence of nucleotide 1 to nucleotide 738 of SEQ ID NO:10.  
     
     
         16 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment encoding a polypeptide of  claim 1;  and    a transcription terminator.    
     
     
         17 . The expression vector of  claim 16  wherein the DNA segment encodes a polypeptide comprising amino acid residues 23-246 of SEQ ID NO:2.  
     
     
         18 . The expression vector of  claim 16  wherein the DNA segment encodes a polypeptide covalently linked at the amino or carboxyl terminus to an affinity tag.  
     
     
         19 . The expression vector of  claim 16  wherein the DNA segment further encodes a secretory signal sequence operably linked to the polypeptide.  
     
     
         20 . The expression vector of  claim 19  wherein the secretory signal sequence comprises amino acid residues 1-22 of SEQ ID NO:2.  
     
     
         21 . A cultured cell into which has been introduced an expression vector of  claim 16  wherein the cell expresses the polypeptide encoded by the DNA segment.  
     
     
         22 . A method of producing a polypeptide comprising: 
 culturing a cell into which has been introduced the expression vector of  claim 16;     whereby the cell expresses the polypeptide encoded by the DNA segment; and    recovering the expressed polypeptide.