Detection of human papillomaviruses

Abstract

A method for detecting human papillomaviruses (HPV) in biological material is described, in which method nucleic acids are extracted/isolated from biological material and HPV-specific DNA is detected in the isolated nucleic acids. This method uses at least one of the nucleotide sequences SEQ ID No. 1 or SEQ ID No. 2 from the enclosed sequence listing or sequences which bind to sequences to which one of the sequences SEQ ID No. 1 or SEQ ID No. 2 binds or sequences which are complementary to the abovementioned sequences.

1 . A method for detecting human papillomaviruses (HPV) in biological material, comprising the steps of: 
 a) extracting/isolating nucleic acids from biological material, and    b) detecting HPV-specific DNA in the isolated nucleic acids, by detecting specific HPV DNA segments using at least one nucleotide sequence out of the group consisting of:    SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3 from the enclosed sequence listing, sequences which bind (hybridize) to sequences to which one of the sequences SEQ ID No. 1. SEQ ID No. 2 or SEQ ID No. 3 binds.    
     
     
         2 . The method of  claim 1 , wherein the detection is effected by way of amplifying the specific HPV-DNA segments by means of a polymerase chain reaction (PCR).  
     
     
         3 . The method of  claim 2 , wherein the PCR is characterized by the following temperature profile:  
       
         
           
                 
                 
                 
               
                     
                     
                 
                     
                     
                 
                     
                   Heating: 
                    5-15 min., 90-100° C.; 
                 
                     
                   Heat denaturation: 
                   30-90 sec., 90-100° C.; 
                 
                     
                   Hybridization: 
                   30-90 sec., 50-60° C.; 
                 
                     
                   Polymerization: 
                   30-180 sec., 67-77° C.; 
                 
                     
                   Cooling: 
                    2-10 min., 67-77° C. 
                 
                     
                     
                 
                     
                     
                 
             
                
                
               
               
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         4 . The method of  claim 2 , wherein the primer pair employed for the PCR comprises the nucleotide sequences SEQ ID No. 1 and SEQ ID No. 2 from the enclosed sequence listing.  
     
     
         5 . The method of  claim 3 , wherein the primer pair employed for the PCR comprises the nucleotide sequences SEQ ID No. 1 and SEQ ID No. 2 from the enclosed sequence listing.  
     
     
         6 . The method of  claim 2 , wherein the primer pair employed for the PCR comprises the nucleotide sequences SEQ ID No. 1 and SEQ ID No. 3 from the enclosed sequence listing.  
     
     
         7 . The method of  claim 3 , wherein the primer pair employed for the PCR comprises the nucleotide sequences SEQ ID No. 1 and SEQ ID No. 3 from the enclosed sequence listing.  
     
     
         8 . The method of  claim 1 , wherein the HPVs are typed by sequencing the amplified HPV DNA segments.  
     
     
         9 . The method of  claim 2 , wherein the HPVs are typed by sequencing the amplified HPV DNA segments.  
     
     
         10 . The method of  claim 3 , wherein the HPVs are typed by sequencing amplified HPV DNA segments.  
     
     
         11 . The method of  claim 2 , as part of a diagnostic method and/or an early recognition method.  
     
     
         12 . The method of  claim 11 , as part of a method for the diagnosis and/or early recognition of cancers, in particular cancer of the uterine cervix.  
     
     
         13 . The nucleotide sequence SEQ ID No. 1 according to the enclosed sequence listing.  
     
     
         14 . The nucleotide sequence SEQ ID No. 2 according to the enclosed sequence listing.  
     
     
         15 . The nucleotide sequence SEQ ID No. 3 according to the enclosed sequence listing.  
     
     
         16 . A nucleotide sequence which binds (hybridizes) to nucleotide sequences to which the nucleotide sequence SEQ ID No. 1 binds.  
     
     
         17 . A nucleotide sequence which binds hybridizes) to sequences to which the nucleotide sequence SEQ ID No. 2 binds.  
     
     
         18 . A nucleotide sequence which binds (hybridizes) to sequences to which the nucleotide sequence SEQ ID No. 3 binds.  
     
     
         19 . A nucleotide sequence which is complementary to the nucleotide sequence of  claim 13 .  
     
     
         20 . A nucleotide sequence which is complementary to the nucleotide sequence of  claim 14 .  
     
     
         21 . A nucleotide sequence which is complementary to the nucleotide sequence of  claim 15 .  
     
     
         22 . A nucleotide sequence which is complementary to the nucleotide sequence of  claim 16 .  
     
     
         23 . A nucleotide sequence which is complementary to the nucleotide sequence of  claim 17 .  
     
     
         24 . A nucleotide sequence which is complementary to the nucleotide sequence of  claim 18 .  
     
     
         25 . A method for detecting, preferably for typing, HPV in the anogenital region, in particular in connection with cancer of the uterine cervix, wherein one or more nucleotide sequences from the HPV E1 open reading frame are used.