Cytosolic phospholipase A2-beta enzymes

Abstract

The invention provides a novel calcium-independent cytosolic phospholipase A 2 -Beta enzyme, polynucleotides encoding such enzyme and methods for screening unknown compounds for anti-inflammatory activity mediated by the arachidonic acid cascade.

1 - 20 . (canceled)  
     
     
         21 . An antibody that binds to a phospholipase enzyme peptide comprising an amino acid sequence encoded by a polynucleotide comprising a nucleotide sequence selected from: 
 (a) the nucleotide sequence of SEQ ID NO: 1;    (b) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2;    (c) a nucleotide sequence encoding a fragment of the amino acid sequence of SEQ ID NO: 2 having activity in a mixed micelle assay with 1-palmitoyl-2-[ 14 C]-arachidonyl-phosphatidylcholine as a substrate;    (d) a nucleotide sequence which hybridizes under stringent conditions of 4×SSC at 65 degrees C with the complement of the sequence of (a), (b) or (c) which encodes a peptide having activity in a mixed micelle assay with 1-palmitoyl-2-[ 14 C]-arachidonyl-phosphatidylcholine as a substrate;    (e) the nucleotide sequence of SEQ ID NO: 3;    (f) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 4;    (g) a nucleotide sequence encoding a fragment of the amino acid sequence of SEQ ID NO: 4 having activity in a mixed micelle assay with 1-palmitoyl-2-[ 14 C]-arachidonyl-phosphatidylcholine as a substrate; and    (h) a nucleotide sequence which hybridizes under stringent conditions of 4×SSC at 65 degrees C with the complement of the sequence of (e), (f) or (g) which encodes a peptide having activity in a mixed micelle assay with 1-palmitoyl-2-[ 14 C]-arachidonyl-phosphatidylcholine as a substrate.    
     
     
         22 . The antibody of  claim 21 , wherein the phospholipase enzyme peptide comprises an amino acid sequence encoded by a polynucleotide comprising a nucleotide sequence which hybridizes under stringent conditions of 4×SSC at 65 degrees C with the complement of a nucleotide sequence selected from: 
 (a) the nucleotide sequence of SEQ ID NO: 1; and    (b) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2.    
     
     
         23 . The antibody of  claim 21 , wherein the phospholipase enzyme peptide comprises an amino acid sequence encoded by a polynucleotide comprising a nucleotide sequence which hybridizes under stringent conditions of 4×SSC at 65 degrees C with the complement of a nucleotide sequence selected from: 
 (a) the nucleotide sequence of SEQ ID NO: 3; and    (b) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 4.    
     
     
         24 . An antibody that binds to a phospholipase enzyme peptide comprising an amino acid sequence selected from: 
 (a) the amino acid sequence of SEQ ID NO: 2; and    (b) a fragment of the amino acid sequence of SEQ ID NO: 2 having activity in a mixed micelle assay with 1-palmitoyl-2-[ 14 C]-arachidonyl-phosphatidylcholine as a substrate.    (c) the amino acid sequence of SEQ ID NO: 4; and    (d) a fragment of the amino acid sequence of SEQ ID NO: 4 having activity in a mixed micelle assay with 1-palmitoyl-2-[ 14 C]-arachidonyl-phosphatidylcholine as a substrate.    
     
     
         25 . The antibody of  claim 24 , wherein the phospholipase enzyme peptide comprises the amino acid sequence of SEQ ID NO: 2.  
     
     
         26 . The antibody of  claim 24 , wherein the phospholipase enzyme peptide comprises a fragment of the amino acid sequence of SEQ ID NO: 2 having activity in a mixed micelle assay with 1-palmitoyl-2-[ 14 C]-arachidonyl-phosphatidylcholine as a substrate.  
     
     
         27 . The antibody of  claim 24 , wherein the phospholipase enzyme peptide comprises the amino acid sequence of SEQ ID NO: 4.  
     
     
         28 . The antibody of  claim 24 , wherein the phospholipase enzyme peptide comprises a fragment of the amino acid sequence of SEQ ID NO: 4 having activity in a mixed micelle assay with 1-palmitoyl-2-[ 14 C]-arachidonyl-phosphatidylcholine as a substrate.  
     
     
         29 . The antibody of  claim 21 , which is a monoclonal antibody.  
     
     
         30 . The antibody of  claim 21 , which is a polyclonal antibody.  
     
     
         31 . The antibody of  claim 24 , which is a monoclonal antibody.  
     
     
         32 . The antibody of  claim 24 , which is a polyclonal antibody.  
     
     
         33 . A composition comprising the antibody of  claim 21  and at least one pharmaceutically acceptable carrier.  
     
     
         34 . A composition comprising the antibody of  claim 24  and at least one pharmaceutically acceptable carrier.