Nucleotide sequences which code for the rpsL gene

Abstract

An isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the rpsL gene is present in enhanced form, as well as the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

1 - 40 . (canceled)  
     
     
         41 . An isolated polynucleotide which consists of at least 20 consecutive bases of a fragment of SEQ ID NO: 1 or a fragment of the full complement of SEQ ID NO: 1.  
     
     
         42 . The polynucleotide of  claim 41 , which consists of a fragment of SEQ ID No: 1.  
     
     
         43 . The polynucleotide of  claim 41 , which consists of a fragment of the full complement of SEQ ID NO: 1.  
     
     
         44 . The polynucleotide of  claim 41  which consists of at least 20 consecutive bases of a fragment between bases 1-499 of SEQ ID NO: 1 or the full complement thereof.  
     
     
         45 . The polynucleotide of  claim 41  which consists of at least 20 consecutive bases of a fragment between bases 500-883 of SEQ ID NO: 1 or the full complement thereof.  
     
     
         46 . The polynucleotide of  claim 41  which consists of at least 20 consecutive bases of a fragment between bases 884-1775 of SEQ ID NO: 1 or the full complement thereof.  
     
     
         47 . The polynucleotide of  claim 41  which consists of at least 30 consecutive bases of a fragment of SEQ ID NO: 1 or the full complement thereof.  
     
     
         48 . The polynucleotide of  claim 41  which consists of at least 50 consecutive bases of a fragment of SEQ ID NO: 1 or the full complement thereof.  
     
     
         49 . The polynucleotide of  claim 41  which consists of at least 100 consecutive bases of a fragment of SEQ ID NO: 1 or the full complement thereof.  
     
     
         50 . A hybridization probe which comprises the polynucleotide of  claim 41 .  
     
     
         51 . An array, microarray or chip to which the polynucleotide of  claim 41  has been applied.  
     
     
         52 . A primer which comprises the polynucleotide of  claim 41 .  
     
     
         53 . A vector comprising the polynucleotide of  claim 42 .  
     
     
         54 . A vector comprising the polynucleotide of  claim 43 .  
     
     
         55 . A host cell transformed with the polynucleotide of  claim 42 .  
     
     
         56 . A host cell transformed with the polynucleotide of  claim 43 .  
     
     
         57 . A method for identifying a polynucleotide sequence which encodes a ribosomal S12 polypeptide comprising: 
 contacting a polynucleotide sample with the probe of  claim 50 ,    isolating a polynucleotide sequence which binds to said probe, and    determining whether said polynucleotide sequence encodes a polypeptide having the activity of the ribosomal S12 polypeptide.    
     
     
         58 . A method for identifying a polynucleotide sequence which encodes a ribosomal S12 polypeptide comprising: 
 contacting a polynucleotide sample with at least one primer of  claim 52  under conditions suitable for amplification of polynucleotides encoding a ribosomal S12 polypeptide by the polymerase chain reaction,    isolating an amplified polynucleotide sequence, and    determining whether said polynucleotide sequence encodes a polypeptide having the activity of the ribosomal S12 polypeptide.    
     
     
         59 . An isolated polynucleotide which consists of at least 20 consecutive bases of a fragment of SEQ ID NO: 3 or a fragment of the full complement of SEQ ID NO: 3.  
     
     
         60 . The polynucleotide of  claim 59 , which consists of a fragment of SEQ ID NO: 3.  
     
     
         61 . The polynucleotide of  claim 59 , which consists of a fragment of the full complement of SEQ ID NO: 3.  
     
     
         62 . The polynucleotide of  claim 59  which consists of at least 30 consecutive bases of a fragment of SEQ ID NO: 3 or the full complement thereof.  
     
     
         63 . The polynucleotide of  claim 59  which consists of at least 50 consecutive bases of a fragment of SEQ ID NO: 3 or the full complement thereof.  
     
     
         64 . The polynucleotide of  claim 59  which consists of at least 100 consecutive bases of a fragment of SEQ ID NO: 3 or the full complement thereof.  
     
     
         65 . A hybridization probe which comprises the polynucleotide of  claim 59 .  
     
     
         66 . An array, microarray or chip to which the polynucleotide of  claim 59  has been applied.  
     
     
         67 . A primer which comprises the polynucleotide of  claim 59 .  
     
     
         68 . A vector comprising the polynucleotide of  claim 60 .  
     
     
         69 . A vector comprising the polynucleotide of  claim 61 .  
     
     
         70 . A host cell transformed with the polynucleotide of  claim 60 .  
     
     
         71 . A host cell transformed with the polynucleotide of  claim 61 .  
     
     
         72 . A method for identifying a polynucleotide sequence which encodes a ribosomal S12 polypeptide comprising: 
 contacting a polynucleotide sample with the probe of  claim 65 ,    isolating a polynucleotide sequence which binds to said probe, and    determining whether said polynucleotide sequence encodes a polypeptide having the activity of the ribosomal S12 polypeptide.    
     
     
         73 . A method for identifying a polynucleotide sequence which encodes a ribosomal S12 polypeptide comprising: 
 contacting a polynucleotide sample with at least one primer of  claim 67  under conditions suitable for amplification of polynucleotides encoding a ribosomal S12 polypeptide by the polymerase chain reaction,    isolating an amplified polynucleotide sequence, and    determining whether said polynucleotide sequence encodes a polypeptide having the activity of the ribosomal S12 polypeptide.