Self-assembling peptide surfaces for cell patterning and interactions

Abstract

This invention describes self assembled monolayers (SAMs) manufactured by imprinting reactive peptides onto solid supports. The invention further relates to methods of preparing and using these improved SAMs.

1 . A device comprising: 
 a solid support; and    an array of isolated regions on the support, the array comprising a layer of peptides, wherein the peptides are bound to the support by a bond between the support and a terminal amino acid in a preselected, reproducible pattern.    
     
     
         2 . The device of  claim 1  further comprising a background region surrounding the isolated regions, wherein the peptides are not bound to the background region.  
     
     
         3 . The device of  claim 2  wherein the background region comprises an inert compound.  
     
     
         4 . A device comprising: 
 a solid support; and    an isolated region comprising a layer of peptides, wherein the peptides are bound to the support by a bond between the support and a terminal reactive group in a preselected, reproducible pattern.    
     
     
         5 . The device of  claim 4  comprising a plurality of isolated regions of a self-assembled monolayer, the plurality of regions defining an ordered array on the support.  
     
     
         6 . A device comprising: 
 a solid support;    an isolated region comprising a self-assembled monolayer of peptides, wherein the peptides are bound to the support by a bond between the support and a terminal reactive group in a preselected, reproducible pattern; and    a background region surrounding the isolated region and comprising a compound which can react with the support.    
     
     
         7 . A device for immobilizing at least one biological material in a specific and predetermined pattern comprising: 
 a surface,    an array of immobilization islands in a specific and predetermined pattern over the surface isolated from each other by at least one background region, the array of immobilization islands comprising a first self-assembled monolayer comprising at least one first functional group wherein the at least one first functional group is selected to biophilic, and wherein the first self-assembled monolayer comprises a monolayer of linear peptides,    the at least one background region comprising a second self-assembled monolayer having a second functional group wherein the second functional group is selected to be biophobic.