Compositions comprising viruses and methods for concentrating virus preparations

Abstract

A composition is disclosed comprising virus in a formulation comprising a polyhydroxy hydrocarbon buffered to maintain a pH in a range from about 7 to about 8.5 at a temperature in the range from about 2° C. to 27° C. Methods for concentrating and purifying virus preparations are also disclosed.

1 - 28 . (canceled)  
     
     
         29 . An adenovirus composition comprising adenovirus particles, sorbitol, and sucrose, wherein the amount of sorbitol and sucrose is sufficient to maintain stability of the adenovirus composition during storage.  
     
     
         30 . The adenovirus composition of  claim 29 , wherein the amount of sorbitol and sucrose is sufficient to maintain the stability of the adenovirus composition when the adenovirus composition is stored at 4° C.  
     
     
         31 . The adenovirus composition of  claim 29 , further comprising Tween 20.  
     
     
         32 . The adenovirus composition of  claim 29 , further comprising Tween 80.  
     
     
         33 . An adenovirus composition comprising adenovirus particles, sorbitol, and Tween 20, wherein the amount of sorbitol and Tween 20 is sufficient to maintain stability of the adenovirus composition during storage.  
     
     
         34 . The adenovirus composition of  claim 31 , wherein the amount of sorbitol and Tween 20 is sufficient to maintain stability of the adenovirus composition when the adenovirus composition is stored at 4° C.  
     
     
         35 . An adenovirus composition comprising adenovirus particles, sorbitol, and Tween 80, wherein the amount of sorbitol and Tween 80 is sufficient to maintain stability of the adenovirus composition during storage.  
     
     
         36 . The adenovirus composition of  claim 35 , wherein the amount of sorbitol and Tween 80 is sufficient to maintain stability of the adenovirus composition when the adenovirus composition is stored at 4° C.  
     
     
         37 . A method for preparing an adenovirus composition, comprising the steps of providing adenovirus particles and combining the adenovirus particles with a solution comprising a buffer, sorbitol, and sucrose, wherein the amount of sorbitol and sucrose is sufficient to maintain stability of the adenovirus composition during storage.  
     
     
         38 . The method of  claim 37 , wherein the amount of sorbitol and sucrose is sufficient to maintain stability of the adenovirus composition when the adenovirus composition is stored at 4° C.  
     
     
         39 . The method of  claim 37 , wherein the adenovirus particles are further combined with Tween 20.  
     
     
         40 . The method of  claim 37 , wherein the adenovirus particles are further combined with Tween 80.