Purified polypeptides having IL-13 receptor activity

Abstract

This invention relates to a purified polypeptide, comprising an amino acid sequence chosen from: a) the sequence SEQ ID No. 2, b) any biologically active sequence derived from SEQ ID No. 2.

1 - 43 . (canceled)  
     
     
         44 . A purified polypeptide comprising an amino acid sequence selected from the group consisting of: 
 (a) the amino acid sequence of SEQ ID NO:2,    (b) the amino acid sequence of SEQ ID NO:2 wherein the 8 c-terminal residues thereof are substituted with the 6 residues VRCVTL of SEQ ID NO: 11,    (c the amino acid sequence 1 to 343 of SEQ ID NO:2, and    (d) the amino acid sequence 1 to 337 of SEQ ID NO:2.    
     
     
         45 . A purified polypeptide comprising the amino acid sequence of SEQ ID NO:2.  
     
     
         46 . A purified polypeptide comprising the amino acid sequence of SEQ ID NO:2 wherein the 8 c-terminal residues thereof are substituted with the 6 residues VRCVTL of SEQ ID NO: 11.  
     
     
         47 . A purified polypeptide in soluble form comprising an amino acid sequence selected from the group consisting of: 
 (a) the amino acid sequence of SEQ ID NO:2 from residues 1 to 343 and    (b) the amino acid sequence of SEQ ID NO:2 from residues 1 to 337.    
     
     
         48 . An isolated IL-13bc (IL-13Rβ) protein comprising an amino acid sequence selected from the group consisting of: 
 (a) the amino acid sequence of SEQ ID NO:2,    (b) the amino acid sequence of SEQ ID NO:2 from amino acids 26 to 341,    (c) the amino acid sequence of SEQ ID NO: 2 from amino acids 363 to 380, and    (d) fragments of (a) to (c) which bind IL-13.    
     
     
         49 . The protein of  claim 48  comprising the sequence from amino acid 26 to 341 of SEQ ID NO: 2.  
     
     
         50 . The protein of  claim 48  comprising the sequence from amino acid sequence 363 to 380 of SEQ ID NO: 2.  
     
     
         51 . The protein of  claim 48  wherein said amino acid sequence is part of a fusion protein.  
     
     
         52 . The isolated protein of  claim 51  comprising an Fc fragment.  
     
     
         53 . A protein produced according to a process comprising: 
 (a) growing a culture of a host cell in a suitable culture medium and    (b) purifying the protein from the culture, wherein said host cell is transformed with a polynucleotide operably linked to an expression control sequence, and wherein said polynucleotide comprises a nucleotide sequence selected from the group consisting of: 
 a. a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:2;  
 b. a nucleotide sequence encoding the IL-13Rβ binding chain varying from the sequence of the nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2 as a result of degeneracy of the genetic code;  
 c. a nucleotide sequence capable of hybridizing under stringent conditions comprising hybridization at 52° C. in 5×SSC followed by washing at 52° C. in 2×SSC to a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2.  
   
     
     
         54 . The protein of  claim 53  wherein said nucleotide sequence is that of a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2.  
     
     
         55 . The protein of  claim 53  wherein said nucleotide sequence encodes the IL-13Rβ binding chain varying from the sequence encoding the amino acid sequence of SEQ ID NO: 2 as a result of the degeneracy of the genetic code.  
     
     
         56 . The protein of  claim 53  wherein said nucleotide sequence is that of a nucleotide sequence capable of hybridizing under stringent conditions comprising hybridization at 52° C. in 5×SSC followed by washing at 52° C. in 2×SSC.  
     
     
         57 . The protein of  claim 53  comprising SEQ ID NO: 2.  
     
     
         58 . An isolated IL-13Rβ protein comprising an amino acid sequence selected from the group consisting of: 
 (e) the amino acid sequence of SEQ ID NO:2,    (f) the amino acid sequence of SEQ ID NO:2 from amino acids 23 to 342,    (g) the amino acid sequence of SEQ ID NO: 2 from amino acids 365 to 380, and    (h) fragments of (a) to (c) which bind IL-13.    
     
     
         59 . The protein of  claim 58  comprising the sequence from amino acid 23 to 342 of SEQ ID NO:2.  
     
     
         60 . The protein of  claim 58  comprising the sequence from amino acid 365 to 380 of SEQ ID NO:2.  
     
     
         61 . An isolated IL-13Rβ protein comprising an amino acid sequence selected from the group consisting of: 
 (a) the amino acid sequence of SEQ ID NO: 2 from amino acids 23 to 380;    (b) the amino acid sequence of SEQ ID NO: 2 from amino acids 23 to 343;    (c) the amino acid sequence of SEQ ID NO: 2 from amino acids 364 to 380; and    (d) fragments of (a) to (c) which bind IL-13.    
     
     
         62 . A protein produced according to a process comprising: 
 (a) growing a culture of a host cell in a suitable culture medium and    (b) purifying the protein from the culture, wherein said host cell is transformed with a polynucleotide operably linked to an expression control sequence and wherein said polynucleotide comprises a nucleotide sequence selected from the group consisting of 
 a. the nucleotide sequence of SEQ ID NO:1 from nucleotide 53 to nucleotide 1192;  
 b. a nucleotide sequence encoding the IL-13Rβ binding chain varying from the sequence of SEQ ID NO: 1 from nucleotide 53 to nucleotide 1192 as a result of degeneracy of the genetic code,  
 c. a nucleotide sequence capable of hybridizing under stringent conditions comprising hybridization at 52° C. in 5×SSC followed by washing at 52° C. in 2×SSC to the nucleotide sequence of SEQ ID NO: 1 from nucleotide 53 to nucleotide 1192.  
   
     
     
         63 . The protein of  claim 62  wherein said nucleotide sequence comprises the nucleotide sequence of SEQ ID NO: 1 from nucleotide 53 to nucleotide 1192.  
     
     
         64 . The protein of  claim 62  wherein said nucleotide sequence comprises a nucleotide sequence encoding the IL-13Rβ binding chain varying from the sequence of SEQ ID NO: 1 from nucleotide 53 to nucleotide 1192 as a result of degeneracy of the genetic code.  
     
     
         65 . The protein of  claim 62  wherein said nucleotide sequence is that of an nucleotide sequence capable of hybridizing under stringent conditions at 52° C. in 5×SSC followed by washing at 52° C. in 2×SSC.