Device for the detection of a fluorescent dye

Abstract

The present invention relates to a device for the detection of a fluorescent dye in a sample, comprising a radiation source means with the aid of which light for exciting the fluorescent dye to be detected can be transmitted into the sample applied to a carrier, and a detecting means for detecting fluorescent light which has been emitted by the fluorescent dye to be detected. The present invention is characterized by a hollow space having an internal high-reflectance surface, a first aperture directed towards the sample, and a second aperture located opposite the detecting means.

1 - 20 . (canceled)  
     
     
         21 . A fluorescence detection system, the system comprising: 
 a sample, wherein the sample comprises a plurality of fluorescent dyes;    multiple radiation sources, wherein each radiation source is configured to transmit light to excite at least one fluorescent dye in the sample; and    at least one detector configured to detect fluorescent light from at least one fluorescent dye in the sample,    wherein the detection system does not have a lens system to collect fluorescent light from the plurality of dyes, and    wherein the detection system does not have a light guide to collect fluorescent light from the plurality of dyes.    
     
     
         22 . The system of  claim 21 , further comprising: 
 a high-reflectance surface, wherein the surface is configured to collect the fluorescent light from the plurality of dyes.    
     
     
         23 . The system of  claim 22 , wherein the surface has a light-tight arrangement with the detector.  
     
     
         24 . The system of  claim 21 , further comprising: 
 multiple dichroic beam dividers, wherein a single dichroic beam divider corresponds to a single radiation source.    
     
     
         25 . The system of  claim 21 , further comprising: 
 a pinhole configured to fluorescence detection of a geometry of the sample.    
     
     
         26 . A method for detecting fluorescent dyes in a biological sample, the method comprising: 
 scanning excitation light of different excitation wavelengths over the carrier in two dimensions;    separating fluorescent light emitted from the carrier from the excitation wavelengths; and    passing the fluorescent light through an aperture; and    detecting the fluorescent light of multiple fluorescent dyes at different wavelengths by arranging multiple detectors with different color filters.    
     
     
         27 . The method of  claim 26 , wherein the separating comprises providing a dichroic beam divider.