Nucleotide sequences which code for the cstA gene

Abstract

The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the cstA gene, and a host-vector system having a coryneform host bacterium in which the cstA gene is present in attenuated form and a vector which carries at least the cstA gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

1 - 30 . (canceled)  
     
     
         31 . A method for the fermentative production of L-amino acids, comprising: 
 a) culturing coryneform bacteria, which include an overexpressed cstA gene comprising the polynucleotide sequence of SEQ ID NO: 1, in a medium suitable for the expression of the cstA gene to thereby produce L-amino acids.    
     
     
         32 . The method according to  claim 31 , further comprising: 
 b) isolating the L-amino acids.    
     
     
         33 . The method as claimed in  claim 31 , wherein the L-amino acid produced is L-lysine.  
     
     
         34 . The method as claimed in  claim 31 , wherein the bacteria comprise, at the same time, one or more endogenous  Corynebacterium glutamicum  genes which are overexpressed, wherein the one or more genes is/are selected from the group consisting of: 
 the gene which encodes feed back resistant aspartate kinase,    the gene which encodes dihydrodipicolinate synthase,    the gene which encodes glycerolaldehyde 3-phosphate dehydrogenase,    the gene which encodes 3-phosphoglycerate kinase,    the gene which encodes pyruyate carboxylase,    the gene which encodes triose phosphate isomerase,    the gene which encodes a protein that exports lysine, and    the gene which encodes the Zwa1 protein.    
     
     
         35 . The method as claimed in  claim 31 , wherein the bacteria comprise, at the same time, one or more endogenous  Corynebacterium glutamicum  genes which are eliminated, wherein said one or more genes is/are selected from the group consisting of: 
 the gene which encodes phosphoenol pyruvate carboxykinase,    the gene which encodes glucose 6-phosphate isomerase,    the gene which encodes pyruvate oxidase, and    the gene which encodes the Zwa2 protein.    
     
     
         36 . The method according to  claim 31 , wherein the bacteria are  Corynebacterium glutamicum.    
     
     
         37 . The method according to  claim 31 , wherein the cstA gene is overexpressed by increasing the copy number of the cstA gene or by operably linking a promoter to the cstA gene.  
     
     
         38 . A method for the fermentative production of L-amino acids, comprising: 
 a) culturing coryneform bacteria, which include an overexpressed cstA gene having a polynucleotide sequence which encodes the polypeptide comprising the amino acid sequence of SEQ ID NO: 2, in a medium suitable for the expression of the cstA gene to thereby produce L-amino acids.    
     
     
         39 . The method according to  claim 38 , further comprising: 
 b) isolating the L-amino acids.    
     
     
         40 . The method as claimed in  claim 38 , wherein the L-amino acid produced is L-lysine.  
     
     
         41 . The method as claimed in  claim 38 , wherein the bacteria comprise, at the same time, one or more endogenous  Corynebacterium glutamicum  genes which are overexpressed, wherein the one or more genes is/are selected from the group consisting of: 
 the gene which encodes feed back resistant aspartate kinase,    the gene which encodes dihydrodipicolinate synthase,    the gene which encodes glycerolaldehyde 3-phosphate dehydrogenase,    the gene which encodes 3-phosphoglycerate kinase,    the gene which encodes pyruvate carboxylase,    the gene which encodes triose phosphate isomerase,    the gene which encodes a protein that exports lysine, and    the gene which encodes the Zwa1 protein.    
     
     
         42 . The method as claimed in  claim 38 , wherein the bacteria comprise, at the same time, one or more endogenous  Corynebacterium glutamicum  genes which are eliminated, wherein said one or more genes is/are selected from the group consisting of: 
 the gene which encodes phosphoenol pyruvate carboxykinase,    the gene which encodes glucose 6-phosphate isomerase,    the gene which encodes pyruvate oxidase, and    the gene which encodes the Zwa2 protein.    
     
     
         43 . The method according to  claim 38 , wherein the bacteria are  Corynebacterium glutamicum.    
     
     
         44 . The method according to  claim 38 , wherein the cstA gene is overexpressed by increasing the copy number of the cstA gene or by operably linking a promoter to the cstA gene.  
     
     
         45 . A method for the fermentative production of L-amino acids, comprising: 
 a) culturing coryneform bacteria, which include an overexpressed cstA gene having a polynucleotide sequence that comprises nucleotides 200 to 2515 of SEQ ID NO: 1, in a medium suitable for the expression of the cstA gene to thereby produce L-amino acids,    wherein the cstA gene is overexpressed by increasing the copy number of the cstA gene or by operably linking a promoter to the cstA gene.    
     
     
         46 . The method according to  claim 45 , further comprising: 
 b) isolating the L-amino acids.    
     
     
         47 . The method as claimed in  claim 45 , wherein the L-amino acid produced is L-lysine.  
     
     
         48 . The method as claimed in  claim 45 , wherein the bacteria comprise, at the same time, one or more endogenous  Corynebacterium glutamicum  genes which are overexpressed, wherein the one or more genes is/are selected from the group consisting of: 
 the gene which encodes feed back resistant aspartate kinase,    the gene which encodes dihydrodipicolinate synthase,    the gene which encodes glycerolaldehyde 3-phosphate dehydrogenase,    the gene which encodes 3-phosphoglycerate kinase,    the gene which encodes pyruvate carboxylase,    the gene which encodes triose phosphate isomerase,    the gene which encodes a protein that exports lysine, and    the gene which encodes the Zwa1 protein.    
     
     
         49 . The method as claimed in  claim 45 , wherein the bacteria comprise, at the same time, one or more endogenous  Corynebacterium glutamicum  genes which are eliminated, wherein said one or more genes is/are selected from the group consisting of: 
 the gene which encodes phosphoenol pyruvate carboxykinase,    the gene which encodes glucose 6-phosphate isomerase,    the gene which encodes pyruvate oxidase, and    the gene which encodes the Zwa2 protein.    
     
     
         50 . A method for the fermentative production of L-amino acids, comprising: 
 a) culturing coryneform bacteria, which include an overexpressed cstA gene having a polynucleotide sequence that comprises a fragment of SEQ ID NO: 1 which encodes the amino acid sequence of SEQ ID NO: 2, in a medium suitable for the expression of the cstA gene to thereby produce L-amino acids,    wherein the cstA gene is overexpressed by increasing the copy number of the cstA gene or by operably linking a promoter to the cstA gene.    
     
     
         51 . The method according to  claim 50 , further comprising: 
 b) isolating the L-amino acids.    
     
     
         52 . The method as claimed in  claim 50 , wherein the L-amino acid produced is L-lysine.  
     
     
         53 . The method as claimed in  claim 50 , wherein the bacteria comprise, at the same time, one or more endogenous  Corynebacterium glutamicum  genes which are overexpressed, wherein the one or more genes is/are selected from the group consisting of: 
 the gene which encodes feed back resistant aspartate kinase,    the gene which encodes dihydrodipicolinate synthase,    the gene which encodes glycerolaldehyde 3-phosphate dehydrogenase,    the gene which encodes 3-phosphoglycerate kinase,    the gene which encodes pyruvate carboxylase,    the gene which encodes triose phosphate isomerase,    the gene which encodes a protein that exports lysine, and    the gene which encodes the Zwa1 protein.    
     
     
         54 . The method as claimed in  claim 50 , wherein the bacteria comprise, at the same time, one or more endogenous  Corynebacterium glutamicum  genes which are eliminated, wherein said one or more genes is/are selected from the group consisting of: 
 the gene which encodes phosphoenol pyruvate carboxykinase,    the gene which encodes glucose 6-phosphate isomerase,    the gene which encodes pyruvate oxidase, and    the gene which encodes the Zwa2 protein.