Transformant containing transaldolase gene

Abstract

The present invention relates to a novel transaldolase gene, and to a polypeptide encoded by the gene, a recombinant DNA obtained by ligating the gene, a transformant carrying the recombinant DNA, and a process for producing the polypeptide, aromatic amino acids, aromatic vitamins, L-histidine, riboflavin, nucleic acids, nucleic acid-associated substances, novel saccharides and others by utilizing the transformant.

1 - 8 . (canceled)  
     
     
         9 . A transformant in which one or more nucleotides have been substituted, deleted or inserted in the nucleotide sequence of the DNA carried by the transformant of  claim 10  or in the nucleotide sequence of a DNA existing upstream the DNA and participating in transcription and translation, and of which the transaldolase activity is enhanced over that of the transformant not having undergone the substitution, deletion or insertion.  
     
     
         10 . A transformant comprising a recombinant DNA obtained by ligating a DNA to a vector said DNA coding for a polypeptide having transaldolase activity and comprising (i) SEQ ID NO:2, (ii) a DNA coding for the amino acid sequence of SEQ ID NO: 1 (iii) a DNA coding for an amino sequence which is at least 60% homologous to SEQ ID NO: 1 and (iv) a DNA hybridizing to any of (i)-(iii) at 62° C. in the presence of 0.7-1.0 mol/l NaCl followed by washing at 65° C. with 0.1-2.0 X SSC, wherein the transformant has an ability to produce an aromatic amino acid or aromatic vitamin.  
     
     
         11 . A process for producing an aromatic amino acid or aromatic vitamin, which comprises: 
 culturing the transformant of  claim 10  in a medium to thereby produce and accumulate in the culture the aromatic amino acid or aromatic vitamin, and    recovering the aromatic amino acid or aromatic vitamin from the culture.    
     
     
         12 . (canceled)  
     
     
         13 . The transformant according to  claim 10 , wherein the transformant has a reduced ability to produce a substance selected from L-histidine, riboflavin and nucleic acids.  
     
     
         14 . A process for producing a substance, which comprises: 
 culturing the transformant of  claim 13  in a medium to thereby produce and accumulate the substance in the culture, and    recovering the substance from the culture.    
     
     
         15 - 16 . (canceled)  
     
     
         17 . A process for producing an aromatic amino acid or aromatic vitamin, which comprises: 
 culturing the transformant of  claim 9  in a medium to thereby produce and accumulate in the culture the aromatic amino acid or aromatic vitamin, and    recovering the aromatic amino acid or aromatic vitamin from the culture.    
     
     
         18 . The transformant according to  claim 9 , wherein the transformant has a reduced ability to produce a substance selected from L-histidine, riboflavin and nucleic acids.  
     
     
         19 . A process for producing a substance, which comprises: 
 culturing the transformant of  claim 18  in a medium to thereby produce and accumulate the substance in the culture, and    recovering the substance from the culture.