Novel polypeptide and its DNA

Abstract

It is intended to construct a screening method, etc. for searching for compounds which are capable of binding to apolipoprotein A-I and thus promote or inhibit the binding of a cubulin fragment to apolipoprotein A-I, and provide preventives or remedies containing compounds obtained by the above screening method, etc. These compounds, etc. are usable in preventives and remedies for various diseases in which cubulin participates and the like.

1 . A polypeptide having the ability to bind to apolipoprotein A-I, which is a partial fragment of cubilin, an amide or ester of said polypeptide, or a salt of said polypeptide.  
     
     
         2 . The polypeptide, amide or ester thereof, or salt thereof of  claim 1 , wherein the polypeptide is characterized by having an amino acid sequence identical or substantially identical with the amino acid sequence as shown in SEQ ID NO: 10.  
     
     
         3 . The polypeptide, amide or ester thereof, or salt thereof of  claim 1 , wherein the polypeptide has the amino acid sequence as shown in SEQ ID NO: 10.  
     
     
         4 - 5 . (canceled)  
     
     
         6 . A DNA comprising a DNA encoding the polypeptide of  claim 1 .  
     
     
         7 . The DNA of  claim 6 , wherein the DNA comprises the base sequence as shown in SEQ ID NO: 9.  
     
     
         8 . The DNA of  claim 6 , wherein the DNA comprises the base sequence as shown in SEQ ID NO: 22.  
     
     
         9 . A recombinant vector comprising the DNA of  claim 6 .  
     
     
         10 . A transformant transformed with the recombinant vector of  claim 9 .  
     
     
         11 . A method of producing the polypeptide, amide or ester thereof, or salt thereof of  claim 1 , comprising: 
 culturing a transformant transformed with a recombinant vector comprising a DNA encoding the polypeptide of  claim 1  and    allowing the transformant to produce the polypeptide.    
     
     
         12 . An antibody to the polypeptide, amide or ester thereof, or salt thereof of  claim 1 .  
     
     
         13 . A method of screening for compounds or salts thereof that promote or inhibit the activity of the polypeptide or salt thereof of  claim 1 , wherein the method is characterized by using the polypeptide, amide or ester thereof, or salt thereof of  claim 1  in a screening assay.  
     
     
         14 . A screening kit for compounds or salts thereof that promote or inhibit the activity of the polypeptide, amide or ester thereof, or salt thereof of  claim 1 , wherein the kit comprises the polypeptide or salt thereof of  claim 1 .  
     
     
         15 . A compound or salt thereof that promotes or inhibits the activity of a polypeptide having the ability to bind to apolipoprotein A-I, which is a partial fragment of cubilin, amide or ester thereof, or salt thereof wherein said compound or salt thereof is obtainable by using the screening method of  claim 13 .  
     
     
         16 . A medicine comprising a compound or salt thereof that promotes or inhibits the activity of a polypeptide having the ability to bind to apolipoprotein A-I, which is a partial fragment of cubilin, amide or ester thereof, or salt thereof wherein said compound or salt thereof is obtainable by using the screening method of  claim 13 .  
     
     
         17 . A prophylactic and/or therapeutic agent for renal disorders, nephritis, nephropathy, proteinuria, nervous disorders or vitamin B 12  deficiency, comprising a compound or salt thereof that promotes or inhibits the activity of a polypeptide having the ability to bind to apolipoprotein A-I, which is a partial fragment of cubilin, amide or ester thereof, or salt thereof wherein said compound or salt thereof is obtainable by using the screening method of  claim 13 .  
     
     
         18 . A prophylactic and/or therapeutic agent for hyperlipemia, hypertriglyceridemia, hypo-high density lipoproteinemia, hypoapolipoproteinemia A-I, after meal hyperlipemia, diabetes, obesity, arteriosclerosis, myocardial infarction or angina, comprising a compound or salt thereof that promotes or inhibits the activity of a polypeptide having the ability to bind to apolipoprotein A-I, which is a partial fragment of cubilin, amide or ester thereof, or salt thereof wherein said compound or salt thereof is obtainable by using the screening method of  claim 13 .  
     
     
         19 . A pharmaceutical composition comprising the polypeptide, amide or ester thereof, or salt thereof of  claim 1  and a pharmacologically acceptable carrier, excipient or diluent.  
     
     
         20 . A prophylactic and/or therapeutic agent for diabetes, obesity, arteriosclerosis, hyperlipemia, hypertriglyceridemia, hypo-high density lipoproteinemia, hypoapolipoproteinemia A-I or nervous disorders, comprising the polypeptide, amide or ester thereof, or salt thereof of  claim 1 .  
     
     
         21 . A diagnostic agent that is characterized by the use of the DNA of  claim 6 .  
     
     
         22 . A diagnostic agent comprising the antibody of  claim 12 .  
     
     
         23 . A method for preventing and/or treating renal disorders, nephritis, nephropathy, proteinuria, nervous disorders or vitamin B 12  deficiency in a mammal, comprising a compound or salt thereof that promotes or inhibits the activity of a polypeptide having the ability to bind to apolipoprotein A-I, which is a partial fragment of cubilin, amide or ester thereof, or salt thereof wherein said compound or salt thereof is obtainable by using the screening method of  claim 13 .  
     
     
         24 . A method for preventing and/or treating hyperlipemia, hypertriglyceridemia, hypo-high density lipoproteinemia, hypoapolipoproteinemia A-I, after meal hyperlipemia, diabetes, obesity, arteriosclerosis, myocardial infarction or angina, characterized by administering to a mammal an effective amount of a compound or salt thereof that promotes or inhibits the activity of a polypeptide having the ability to bind to apolipoprotein A-I, which is a partial fragment of cubilin, amide or ester thereof, or salt thereof wherein said compound or salt thereof is obtainable by using the screening method of  claim 13 .  
     
     
         25 . A method for manufacturing a prophylactic and/or therapeutic agent for treating or preventing, in a mammal in need thereof, hyperlipemia, hypertriglyceridemia, hypo-high density lipoproteinemia, hypoapolipoproteinemia A-I, after meal hyperlipemia, diabetes, obesity, arteriosclerosis, myocardial infarction or angina, comprising 
 combining a therapeutic amount of a compound or salt thereof that promotes or inhibits the activity of a polypeptide having the ability to bind to apolipoprotein A-I, which is a partial fragment of cubilin, amide or ester thereof, or salt thereof, wherein said compound or salt thereof is obtainable by using the screening method of  claim 13  with    a pharmacologically acceptable carrier, excipient or diluent.    
     
     
         26 . A method for preventing and/or treating diabetes, obesity, arteriosclerosis, hyperlipemia, hypertriglyceridemia, hypo-high density lipoproteinemia, hypoapolipoproteinemia A-I or nervous disorders, characterized by administering to a mammal an effective amount of the polypeptide, amide or ester thereof, or salt thereof of  claim 1 .  
     
     
         27 . A method for manufacturing a prophylactic and/or therapeutic agent for treating or preventing, in a mammal in need thereof, diabetes, obesity, arteriosclerosis, hyperlipemia, hypertriglyceridemia, hypo-high density lipoproteinemia, hypoapolipoproteinemia A-I or nervous disorders, comprising 
 combining a therapeutic amount of a compound or salt thereof that promotes or inhibits the activity of a polypeptide having the ability to bind to apolipoprotein A-I, which is a partial fragment of cubilin, amide or ester thereof, or salt thereof with    a pharmacologically acceptable carrier, excipient or diluent.