Biocontrol for plants with bacillus subtilis, pseudomonas putida, and sporobolomyces roseus

Abstract

The present invention is directed to isolated Bacillus subtilis, Pseudomonas putida , and Sporobolomyces roseus which are useful as a biocontrol agent. These organisms are useful in a method of imparting to plants protection against plant pathogens by applying them to plants, plant seeds, or soil surrounding plants under conditions effective to impart disease protection to the plants or plants produced from the plant seeds. The biocontrol agents are also useful in a method of enhancing plant growth which involves applying them to plants, plants seeds, or soil surrounding plants under conditions effective to enhance growth in the plants or plants produced from the plant seeds.

1 . A supernatant obtained from a culture of a biocontrol agent comprising an isolated  Bacillus subtilis  having ATCC Accession No. 202152, wherein said supernatant comprises an antifungal metabolite produced by said biocontrol agent.  
   
   
       2 . A supernatant obtained from a culture of a biocontrol agent comprising an isolated  Bacillus subtilis  having a distinctive ribotype pattern, wherein said supernatant comprises an antifungal metabolite produced by said biocontrol agent, and wherein said distinctive ribotype pattern comprises: 
 a first peak having a relative band intensity (“RBI”) of between about 204 and 306 and an estimated molecular weight of between about 1.70 and 2.70 kilobase pairs (kb);    a second peak having an RBI of between about 168 and 252 and a molecular weight of between about 3.60 and 4.60 kb;    a third peak having an RBI of between about 72 and 108 and a molecular weight of between about 5.10 and 6.10 kb;    a fourth peak having an RBI of between about 116 and 174 and a molecular weight of between about 5.60 and 6.60 kb;    a fifth peak having an RBI of between about 116 and 174 and a molecular weight of between about 5.70 and 6.70 kb;    a sixth peak having an RBI of between about 84 and 126 and a molecular weight of between about 8.00 and 9.00 kb; and    a seventh peak having an RBI of between about 78 and 118 and a molecular weight of between about 11.00 and 12.17 kb,    and wherein said RBI is a value on a scale from 0 to 255.    
   
   
       3 . The supernatant according to  claim 2 , wherein said distinctive ribotype pattern comprises: 
 a first peak having an RBI of approximately 255 and an estimated molecular weight of approximately 2.17 kb;    a second peak having an RBI of approximately 210 and a molecular weight of approximately 4.12 kb;    a third peak having an RBI of approximately 90 and a molecular weight of approximately 5.61 kb;    a fourth peak having an RBI of approximately 145 and a molecular weight of approximately 6.13 kb;    a fifth peak having an RBI of approximately 145 and a molecular weight of approximately 6.24 kb;    a sixth peak having an RBI of approximately 105 and a molecular weight of approximately 8.53 kb; and    a seventh peak having an RBI of approximately 98 and a molecular weight of approximately 11.59 kb.    
   
   
       4 . The supernatant according to  claim 2 , wherein the ribotype pattern is substantially similar to that delineated in Table 1 of the present application.