Antibodies and peptide antigens for producing antibodies having a selective binding specificity to bioactive intact parathyroid hormone (PTH) 1-84

Abstract

Peptide antigens corresponding to amino acid residues 2-12, 1-12, 2-15 and 1-15 of parathyroid hormone (PTH), antibodies having an affinity to such peptide antigens and methods of producing the same. Such antigens, antibodies and methods producing the same according to the present invention are useful in determining bioactive intact PTH levels in serum, plasma, and/or cell culture media. Such antibodies further possess a high degree of species cross-reactivity, but substantially mitigated cross-reactivity to non-whole PTH peptide fragments and little to no recognition of the first amino acid residue of PTH.

1 - 24 . (canceled)  
     
     
         25 . An antibody having an affinity to the N-terminal portion of (1-84) PTH, said antibody being produced by a method comprising the steps: 
 a) administering a first peptide antigen to a host animal to induce antibody production against said first peptide antigen in said host animal, said first peptide antigen being selected from the group consisting of SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, (1-34) PTH and (1-84) PTH;    b) monitoring antibody titer produced by said administration of said at least one antigen to said host animal;    c) extracting antisera produced in said host animal by said administration of said at least one peptide antigen; and    d) isolating and selecting at least one antibody from said antisera extracted in step c) by affinity chromatography utilizing a second peptide antigen selected from the group consisting of SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, and SEQ ID NO.6.    
     
     
         26 . A test kit used for the determination of bioactive intact (1-84) PTH utilizing the antibody of  claim 25 .  
     
     
         27 . The antibody of  claim 25  wherein said antibody further includes a detectable moiety.  
     
     
         28 . The antibody of  claim 27  wherein said moiety is selected from the group consisting of a radioactive tracer, a fluorescent tracer, an enzymatic tracer and a dye tracer.