Reagents and methods useful for detecting diseases of the prostate

Abstract

A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as PS108 and transcribed from prostate tissue, is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases and conditions of the prostate, such as prostate cancer. Also provided are antibodies which specifically bind to PS108-encoded polypeptide or protein, and agonists or inhibitors which prevent action of the tissue-specific PS108 polypeptide, which molecules are useful for the therapeutic treatment of prostate diseases, tumors or metastases.

1 - 9 . (canceled)  
     
     
         10 . A test kit useful for detecting a polynucleodie in a test sample, said test kit comprising a container containing at least one polynucleotide having at least 50% identity with a sequence selected from the group consisting of SEQUENCE ID NOS: 1-16, and fragments or complements thereof, wherein said fragments have a length of at least 10 nucleotides.  
     
     
         11 . A purified polynucleotide or fragment thereof having at least 50% identity with a sequence selected from the group consisting of SEQUENCE ID NOS: 1-16, and fragments or complements thereof, wherein said fragments have a length of at least 10 nucleotides.  
     
     
         12 . The purified polynucleotide of  claim 11 , wherein said polynucleotide is produced by recombinant techniques.  
     
     
         13 . The purified polynucleotide of  claim 11 , wherein said polynucleotide is produced by synthetic techniques.  
     
     
         14 . The purified polynucleotide of  claim 11 , wherein said polynucleotide comprises a sequence encoding at least one epitope.  
     
     
         15 . A recombinant expression system comprising a nucleic acid sequence that includes an open reading frame operably linked to a control sequence compatible with a desired host, wherein said nucleic acid sequence has at least 50% identity with a sequence selected from the group consisting of SEQUENCE ID NOS: 1-16, and fragments or complements thereof, wherein said fragments have a length of at least 10 nucleotides.  
     
     
         16 . A cell transfected with the recombinant expression system of  claim 15 .  
     
     
         17 - 32 . (canceled)  
     
     
         33 . A composition of matter comprising a polynucleotide or fragment thereof, wherein said polynucleotide or fragment thereof, wherein said polynucleotide has at least 50% identity with a polynucleotide selected from the group consisting of SEQUENCE ID NOS: 1-16, and fragments or complements thereof, wherein said fragments have a length of at least 10 nucleotides.  
     
     
         34 . (canceled)  
     
     
         35 . A test kit of  claim 10  further comprising a container with tools useful for collection of said sample, wherein the tools are selected from the group consisting of lancets, absorbent paper, cloth, swabs and cups.  
     
     
         36 - 37 . (canceled)  
     
     
         38 . A polynucleotide that codes for a protein comprising an amino acid sequence having at least 50% identity to SEQUENCE ID NO: 36.  
     
     
         39 . A polynucleotide comprising DNA having at least 50% identity with SEQUENCE ID NO: 15 or SEQUENCE ID NO: 16.