Methods of donor specific crossmatching

Abstract

The detection of endothelial cell antibodies has been proven clinically important for successful organ transplantation. Disclosed are methods of isolating endothelial cell antibodies for donor-specific crossmatching.

1 . A kit for isolating an endothelial cell precursor cell in a sample comprising a first antibody that specifically binds an endothelial cell precursor cell surface marker and a detection reagent.  
     
     
         2 . The kit of  claim 1 , wherein said endothelial cell precursor cell surface marker is Tie2, HLA II, CD31, CD105, CD151, VEGFR-2, CD133, VEGFR-1, CD68, CD14, CD62E, CD106, CD54, VWF or Ac-LDL.  
     
     
         3 . The kit of  claim 1 , wherein the first antibody is a polyclonal antibody.  
     
     
         4 . The kit of  claim 1 , wherein the first antibody is a monoclonal.  
     
     
         5 . The kit of  claim 1 , wherein the kit further comprises written instructions for using the kit to determine whether a test sample contains an endothelial cell precursor cell.  
     
     
         6 . The kit according to  claim 1 , wherein the kit further comprises a positive control that comprises a polypeptide that comprises an antigenic determinant of an endothelial cell precursor cell surface marker protein, wherein the antigenic determinant binds to said first antibody.  
     
     
         7 . The kit according to  claim 1 , wherein the kit further comprises a negative control that comprises a polypeptide that does not comprises an antigenic determinant of an endothelial cell precursor cell surface marker protein.  
     
     
         8 . The kit of  claim 1 , wherein said sample is blood.  
     
     
         9 . The kit of  claim 1 , wherein said kit further comprises a sample collection means.  
     
     
         10 . The kit of  claim 9 , wherein said sample collection means further comprises an anti-coagulant.  
     
     
         11 . The kit of  claim 10 , wherein said anti-coagulant is heparin.  
     
     
         12 . The kit of  claim 9 , wherein said sample collection means is a CPT vacutainer tube.  
     
     
         13 . The kit of  claim 1 , wherein said detection reagent is a second antibody.  
     
     
         14 . The kit of  claim 13 , where said second antibody specifically binds human immunoglobin.  
     
     
         15 . The kit of  claim 14 , wherein said second antibody specifically binds an IgG or IgM immunoglobin.  
     
     
         16 . The kit of  claim 13 , wherein the second antibody is a polyclonal antibody.  
     
     
         17 . The kit of  claim 13 , wherein the second antibody is a monoclonal antibody.  
     
     
         18 . The kit of  claim 1 , wherein the first antibody is immobilized on a solid support.  
     
     
         19 . The kit of  claim 18 , wherein said solid support is, a particle, a microtiter plate, a cuvette, a tube, or a strip.  
     
     
         20 . The kit of  claim 19 , wherein said particle is a microparticle.  
     
     
         21 . The kit of  claim 20 , wherein said microparticle is a paramagnetic particle.