US2005239141A1PendingUtilityA1

Modified antibody variable domains

57
Individually held — no corporate assignee on recordPriority: Dec 13, 1991Filed: May 19, 2005Published: Oct 27, 2005
Est. expiryDec 13, 2011(expired)· nominal 20-yr term from priority
Inventors:Gary M. Studnicka
A61P 7/06A61P 5/14A61P 43/00A61P 37/06A61P 9/04A61P 37/02A61P 3/10A61P 31/04A61P 25/00A61P 27/02A61P 29/00A61P 1/04C07K 2317/24C07K 2317/74A61K 39/39516C07K 16/467A61P 21/04A61K 38/00A61K 2039/505A61P 17/12C07K 16/2866A61P 17/06G16B 15/00A61P 1/16C07K 16/465C07K 2319/00A61P 19/02G16B 30/00A61K 39/395C07K 16/2896G01N 33/6854G16B 30/10G16B 20/00G16B 15/20G16B 20/30
57
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Claims

Abstract

Methods are described for identifying the amino acid residues of an antibody variable domain which may be modified without diminishing the native affinity of the domain for antigen while reducing its immunogenicity with respect to a hereterologous species and for preparing so modified antibody variable domains which are useful for administration to heterologous species. Antibody variable regions prepared by the methods of the invention are also described.

Claims

exact text as granted — not AI-modified
1 . A method for determining the risk of changing an amino acid residue in an antibody variable domain, said method comprising 
 (a) aligning by computer the amino acid sequence of said variable domain with the amino acid sequence of an antibody light or heavy chain variable region or consensus sequence; and    (b) determining the risk assigned to changing said amino acid residue using the paired bind and bury lines as shown in  FIG. 6A  or  6 B.    
     
     
         2 . The method of  claim 1 , wherein said selected antibody variable region in part (b) is said light chain variable region.  
     
     
         3 . The method of  claim 1 , wherein said selected antibody variable region in part (b) is said heavy chain variable region.  
     
     
         4 . A method for determining the risk of changing an amino acid residue in an antibody variable region light chain sequence, said method comprising 
 (a) aligning by computer the amino acid sequence of said antibody variable domain light chain sequence with the amino acid sequence of an antibody light chain variable region sequence or consensus; and    (b) determining the risk assigned to changing said amino acid residue using the paired bind and bury lines as shown in  FIG. 6A .    
     
     
         5 . A method for determining the risk of changing an amino acid residue in an antibody variable region heavy chain sequence, said method comprising 
 (a) aligning by computer the amino acid sequence of said antibody variable domain heavy chain sequence with the amino acid sequence of an antibody heavy chain variable region sequence or consensus; and    (b) determining the risk assigned to changing said amino acid residue using the paired bind and bury lines as shown in  FIG. 6B .

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