13245, a novel human myotonic dystrophy type protein kinase and uses therefor

Abstract

The invention provides isolated nucleic acids molecules, designated 13245 nucleic acid molecules, which encode a novel myotonic dystrophy type protein kinase. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 13245 nucleic acid molecules, host cells into which the expression vectors have been introduced, and non-human transgenic animals in which a 13245 gene has been introduced or disrupted. The invention still further provides isolated 13245 proteins, fusion proteins, antigenic peptides and anti-13245 antibodies. Diagnostic methods utilizing compositions of the invention are also provided.

1 - 7 . (canceled)  
     
     
         8 . An isolated polypeptide selected from the group consisting of: 
 a) a polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to the nucleotide sequence of either of SEQ ID NOs: 1 and 3, wherein the polypeptide has kinase activity;    b) a polypeptide which is encoded by a nucleic acid molecule comprising a fragment of at least 1400 nucleotides of the nucleotide sequence of either of SEQ ID NO: 1 or SEQ ID NO:3, wherein the fragment has kinase activity;    c) a polypeptide comprising the amino acid sequence of SEQ ID NO: 2;    d) a polypeptide comprising an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO:2, wherein the polypeptide has kinase activity; and    e) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, wherein the fragment comprises at least 1000 contiguous amino acids of SEQ ID NO: 2; wherein the fragment has kinase activity.    
     
     
         9 . The isolated polypeptide of  claim 8  comprising the amino acid sequence of SEQ ID NO: 2.  
     
     
         10 . The polypeptide of  claim 8 , further comprising a heterologous amino acid sequence.  
     
     
         11 - 49 . (canceled)  
     
     
         50 . An isolated polypeptide selected from the group consisting of: 
 a) a polypeptide which is encoded by a nucleic acid molecule consisting of a nucleotide sequence which is at least 95% identical to the nucleotide sequence of either of SEQ ID NOs: 1 and 3, wherein the polypeptide has kinase activity;    b) a polypeptide fragment which is encoded by a nucleic acid molecule consisting of a fragment of at least 1400 nucleotides of the nucleotide sequence of either of SEQ ID NO: 1 or SEQ ID NO:3, wherein the polypeptide fragment has kinase activity;    c) a polypeptide consisting of the amino acid sequence of SEQ ID NO: 2;    d) a polypeptide consisting of an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO:2, wherein the polypeptide has kinase activity. and    e) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, wherein the fragment consists of at least 1000 contiguous amino acids of SEQ ID NO: 2; wherein the fragment has kinase activity.    
     
     
         51 . The isolated polypeptide of  claim 50  consisting of the amino acid sequence of SEQ ID NO: 2.  
     
     
         52 . The isolated polypeptide of  claim 8 , wherein the polypeptide is encoded by the nucleotide sequence comprising SEQ ID NO: 3.  
     
     
         53 . The isolated polypeptide of  claim 50 , wherein the polypeptide is encoded by the nucleotide sequence consisting of SEQ ID NO: 3.