Adjuvant preparation for the induction of specific immunity

Abstract

Disclosed are methods and compositions useful in the recruitment, activation and initiation of proliferation of immune cells. The instant invention relates to the discovery that specific insect cells and insect cell compositions exhibit adjuvant properties. The added benefit of this system is that the insect cells utilized may be transformed with an expression system and thus proteins may be introduced into the composition through direct protein expression by the cells. The claimed compositions and methods are particularly relevant in anti-tumor and cancer therapy. Of specific interest is the ability of the compositions of the invention to elicit a response that controls not only the primary tumor but also any metastatic cells or metastatic tumors which subsequently arise.

1 - 132 . (canceled)  
   
   
       133 . A method of inhibiting cancer growth in a host having a cancer comprising: 
 (a) isolating cancer cells from said host;    (b) rendering said cancer cells inactive;    (c) reintroducing said inactivated cancer cells into said host in a pharmaceutical composition, said pharmaceutical composition further comprising an insect cell composition.    
   
   
       134 . The method of  claim 133 , wherein said insect cell composition further comprises an immunomodulator.  
   
   
       135 . The method of  claim 134 , wherein said immunomodulator is IFNα.  
   
   
       136 . The method of  claim 134 , wherein said immunomodulator is IL-2.  
   
   
       137 . The method of  claim 133 , wherein said cancer is localized.  
   
   
       138 . The method of  claim 133 , wherein said cancer is metastatic.  
   
   
       139 . The method of  claim 140 , wherein said exogenous DNA is a baculovirus expression vector.  
   
   
       140 . The method of  claim 133 , wherein said insect cell composition comprises an exogenous DNA.  
   
   
       141 . The method of  claim 140 , wherein said exogenous construct encodes an immunomodulator.  
   
   
       142 - 194 . (canceled)