Purification method

Abstract

The invention relates to a method of removing endotoxin from preparation of alpha-1-acid glycoprotein (orosomucoid) by contact with a finely divided non-toxic resin such as fumed silica. The invention also relates to a purification process for alpha-1-acid glycoprotein which includes this depyrogenation step, and to the depyrogenated product and its clinical uses.

1 - 26 . (canceled)  
   
   
       27 . A method of treating drug toxicity in a patient in need of such treatment comprising administering to the patient an effective amount of alpha-1-acid glycoprotein (AAG), wherein the AAG comprises less than or equal to 0.1 Eu lipopolysaccharide per mg AAG.  
   
   
       28 . The method of  claim 27 , wherein the dose of AAG administered is about 10 g to about 30 g.  
   
   
       29 . The method of  claim 27 , wherein the toxicity is caused by a drug overdose.  
   
   
       30 . The method of  claim 29 , wherein the drug comprises a tricyclic anti-depressant.  
   
   
       31 . The method of  claim 30 , wherein the tricyclic antidepressant is amitriptyline, desipramine, or nortiptyline.  
   
   
       32 . The method of  claim 29 , wherein the drug comprises a basic drug.  
   
   
       33 . The method of  claim 32 , wherein the basic drug is quinine, lignocaine or propranolol.  
   
   
       34 . The method of  claim 27 , wherein the AAG is administered enterally, parenterally, by transmucosal delivery, by an implant, intravenously, intramuscularly or subcutaneously.