US2005227236A1PendingUtilityA1

Mold infections

60
Individually held — no corporate assignee on recordPriority: Feb 10, 2003Filed: Feb 10, 2004Published: Oct 13, 2005
Est. expiryFeb 10, 2023(expired)· nominal 20-yr term from priority
Inventors:Neil E. ForsbergSteven B. Puntenney
C12Q 1/6895C12Q 2600/16
60
PatentIndex Score
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Cited by
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References
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Claims

Abstract

Isolated nucleic acids, kits and methods are disclosed for detecting, quantifying and/or identifying a fungal species in a sample or for diagnosing jejunal hemorrhage syndrome in a subject. The methods generally include amplifying a fungal nucleic acid sequence present in the sample using a primer selected from the group consisting of: SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 11, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 or SEQ. ID NO 16; and observing or measuring the amplified nucleic acid sequence. The fungal species can thereby be detected, quantified, and/or identified. Fungal contamination also can be detected or measured in a biological or feed or food sample.

Claims

exact text as granted — not AI-modified
1 . A method of detecting a fungal species in a sample, comprising: 
 amplifying a fungal nucleic acid sequence in the sample using a primer selected from the group consisting of: SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 11, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 and SEQ. ID NO 16; and    observing an amplified fungal nucleic acid sequence.    
     
     
         2 . The method of  claim 1 , further comprising: 
 amplifying a fungal nucleic acid sequence in the sample using a primer selected from the group consisting of: SEQ. ID NO 1, SEQ. ID NO 2, SEQ. ID NO 3, SEQ. ID NO 4, and SEQ. ID NO 15.    
     
     
         3 . The method of  claim 1  wherein the sample is a feed or food sample.  
     
     
         4 . The method of  claim 1  wherein the sample is a livestock feed or food sample.  
     
     
         5 . The method of  claim 1  wherein the sample is a biological sample from a subject.  
     
     
         6 . The method of  claim 1  wherein the subject is a human.  
     
     
         7 . The method of  claim 1  wherein the subject is livestock.  
     
     
         8 . The method of  claim 1  wherein the subject is a ruminant.  
     
     
         9 . The method of  claim 1  wherein the subject is suspected of having jejunal hemorrhage syndrome.  
     
     
         10 . The method of  claim 1 , further comprising: 
 quantifying the amplified fungal nucleic acid sequence.    
     
     
         11 . The method of  claim 1 , further comprising: 
 identifying a detected fungal species by correlating the observed fungal nucleic acid sequence with a fungal species.    
     
     
         12 . The method of  claim 1  wherein observing the amplified nucleic acid sequence comprises measuring the amplified nucleic acid sequence.  
     
     
         13 . A method of diagnosing jejunal hemorrhage syndrome in a subject, comprising: 
 amplifying a fungal nucleic acid sequence present in a subject biological sample using a primer selected from the group consisting of: SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 11, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 and SEQ. ID NO 16;    observing the amplified fungal nucleic acid sequence; and    diagnosing jejunal hemorrhage syndrome in the subject.    
     
     
         14 . The method of  claim 13 , further comprising: 
 amplifying a fungal nucleic acid sequence in the sample using a primer selected from the group consisting of: SEQ. ID NO 1, SEQ. ID NO 2, SEQ. ID NO 3, SEQ. ID NO 4, and SEQ. ID NO 15.    
     
     
         15 . The method of  claim 13  wherein observing the amplified fungal nucleic acid sequence comprises measuring the amplified fungal nucleic acid sequence.  
     
     
         16 . A method of diagnosing jejunal hemorrhage syndrome in a subject, comprising: 
 amplifying a fungal nucleic acid sequence present in a sample of the subject's feed or food using a primer selected from the group consisting of: SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 1, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 and SEQ. ID NO 16;    observing the amplified nucleic acid sequence; and    diagnosing jejunal hemorrhage syndrome in the subject.    
     
     
         17 . The method of  claim 16 , further comprising: 
 amplifying a fungal nucleic acid sequence in the sample using a primer selected from the group consisting of: SEQ. ID NO 1, SEQ. ID NO 2, SEQ. ID NO 3, SEQ. ID NO 4, and SEQ. ID NO 15.    
     
     
         18 . The method of  claim 16  wherein observing the amplified nucleic acid sequence comprises measuring the amplified nucleic acid sequence.  
     
     
         19 . A method of detecting fungal contamination in a feed or food sample. The method includes amplifying a fungal nucleic acid sequence present in a feed or food sample using a primer selected from the group consisting of: SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 1, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 and SEQ. ID NO 16; and 
 observing or measuring the amplified nucleic acid sequence.    
     
     
         20 . The method of  claim 19 , further comprising: 
 amplifying a fungal nucleic acid sequence in the sample using a primer selected from the group consisting of: SEQ. ID NO 1, SEQ. ID NO 2, SEQ. ID NO 3, SEQ. ID NO 4, and SEQ. ID NO 15.    
     
     
         21 . The method of  claim 19 , further comprising: 
 measuring fungal contamination in the feed or food sample.    
     
     
         22 . The method of  claim 19  wherein the feed or food is livestock feed or food.  
     
     
         23 . An isolated nucleic acid comprising: 
 a nucleic acid sequence at least 85% identical to the nucleic acid sequence set forth as SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 11, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 and SEQ. ID NO 16.    
     
     
         24 . The isolated nucleic acid of  claim 23 , comprising: 
 a nucleic acid sequence at least 90% identical to the nucleic acid sequence set forth as SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 11, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 and SEQ. ID NO 16.    
     
     
         25 . The isolated nucleic acid of  claim 23 , comprising: 
 a nucleic acid sequence at least 95% identical to the nucleic acid sequence set forth as SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 11, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 and SEQ. ID NO 16.    
     
     
         26 . The isolated nucleic acid of  claim 23 , comprising: 
 a nucleic acid sequence set forth as SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 11, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 and SEQ. ID NO 16.    
     
     
         27 . A kit for detecting or measuring fungal contamination in a sample, comprising: 
 a container; and    a primer selected from the group consisting of: SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 11, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 and SEQ. ID NO 16.    
     
     
         28 . The kit of  claim 27 , further comprising: 
 a primer selected from the group consisting of: SEQ. ID NO 1, SEQ. ID NO 2, SEQ. ID NO 3, SEQ. ID NO 4, and SEQ. ID NO 15.    
     
     
         29 . The kit of  claim 27  wherein the sample is a biological sample.  
     
     
         30 . The kit of  claim 27  wherein the sample is a feed or food sample.  
     
     
         31 . The kit of  claim 27 , further comprising: 
 a preservative.    
     
     
         32 . The kit of  claim 27 , further comprising: 
 use instructions.    
     
     
         33 . A kit for diagnosing jejunal hemorrhage syndrome, comprising: 
 a container; and    a primer selected from the group consisting of: SEQ. ID NO 5, SEQ. ID NO 6, SEQ. ID NO 7, SEQ. ID NO 8, SEQ. ID NO 9, SEQ. ID NO 10, SEQ. ID NO 11, SEQ. ID NO 12, SEQ. ID NO 13, SEQ. ID NO 14 and SEQ. ID NO 16.    
     
     
         34 . The kit of  claim 33 , further comprising: 
 a primer selected from the group consisting of: SEQ. ID NO 1, SEQ. ID NO 2, SEQ. ID NO 3, SEQ. ID NO 4, and SEQ. ID NO 15.    
     
     
         35 . The kit of  claim 33 , further comprising: 
 a preservative.    
     
     
         36 . The kit of  claim 33 , further comprising: 
 use instructions.

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