Immunogen adherence inhibitor and method of making and using same

Abstract

A microbial adherence inhibitor in the form of fowl egg antibodies is disclosed, along with the method of making it and methods of using it. The inhibitor functions by substantially preventing the attachment or adherence of colony-forming immunogens in the rumen and intestinal tracts of host food animals. The inhibitor is made by inoculating female birds with the immunogen, harvesting the eggs which contain antibodies to the immunogen, harvesting the eggs which contain antibodies to the immunogen, drying the egg contents and adding to the feed or water for the host animals. Dependent upon the particular immunogen with which the female bird is inoculated, the egg antibody is used to promote the growth of food animals by improving feed conversion rates by decreasing the waste of dietary protein caused by the presence of certain colony-forming organisms in the animals, and to substantially reduce or eliminate the incidence of illnesses caused by the presence of certain illness-causing colony-forming immunogens, such as E. coli 0157:H7, in meat from food animals, and in other food stuffs.

1 . A method for reducing or eliminating the incidence of illnesses in humans caused by the presence of targeted colony-forming illness-causing immunogens from the class consisting of  E. coli, Listeria, Salmonella  and  Campylobacter  in meat by inhibiting the ability of the immunogens to adhere to the rumen or intestinal tracts of food animals to reduce the ability of the immunogens to multiply in the rumen or intestinal tracts of the food animals, said method comprising: 
 A. Inoculating female chickens, in or about to reach their egg laying age, with a targeted colony-forming illnesses-causing immunogen from the class consisting of  E. coli Listeria, Salmonella  and  Campylobacter;      B. Allowing a period of time sufficient to permit the production in the eggs of the chickens of antibody to the targeted colony-forming illness-causing immunogen, said antibody in the eggs including IgY immunoglobulins in the yolks of the eggs and IgM and IgA immunoglobulins in the albumin of the eggs;    C. Harvesting the eggs laid by the chickens;    D. Separating the entire contents of said harvested eggs from the shells;    E. Drying said separated entire contents of said eggs to provide a dried egg antibody product;    F. Mixing the resulting dried egg antibody product substantially uniformly through an animal feed or water; and    G. Supplying the resulting mixed dried egg antibody product and animal feed or water to food animals whereby the IgY immunoglobulins bind to the targeted colony-forming illness-causing immunogen, said binding being assisted by the IgM and IgA immunoglobulins to inhibit adherence of the targeted colony-forming illness-causing immunogen in the intestinal tract of the food animals thereby reducing or eliminating the incidence of food borne illnesses in humans caused by the presence of the targeted colony-forming illness-causing immunogen in meat.    
     
     
         2 . A method for reducing or eliminating the incidence of illnesses in humans caused by the presence of targeted colony-forming illness-causing immunogen from the class consisting of  E. coli, Listeria, Salmonella  and  Campylobacter  in meat by inhibiting the ability of the immunogen to adhere to the rumen or intestinal tracts of food animals to reduce the ability of the immunogen to multiply in the rumen or intestinal tracts of the food animals, said method comprising: 
 A. Inoculating female chickens, in or about to reach their egg laying age, with a particular targeted colony-forming illnesses-causing immunogen from the class consisting of  E. coli, Listeria, Salmonella  and  Campylobacter;      B. Allowing a period of time sufficient to permit the production in the eggs of the chickens of antibody to the targeted colony-forming illness-causing immunogen, said antibody in the eggs including IgY immunoglobulins in the yolks of the eggs and IgM and IgA immunoglobulins in the albumin of the eggs;    C. Harvesting the eggs laid by the chickens;    D. Separating the entire contents of said harvested eggs from the shells;    E. Drying said separated entire contents of said eggs to provide a dried egg antibody product;    F. Providing a dry feed carrier;    G. Coating the dry feed carrier with said egg antibody product;    H. Mixing the resulting dry feed carrier coated with the dried egg antibody product substantially uniformly through an animal feed or water; and    I. Supplying the resulting mixed dry feed carrier coated with dried egg antibody product and animal feed or water to food animals whereby the IgY immunoglobulins bind to the targeted colony-forming illness-causing immunogen, said binding being assisted by the IgM and IgA immunoglobulins to inhibit adherence of the targeted colony-forming illness-causing immunogen in the intestinal tract of the food animals thereby reducing or eliminating the incidence of food borne illnesses in humans caused by the presence of the targeted colony-forming illness-causing immunogen in meat.    
     
     
         3 . A method for reducing or eliminating the incidence of illnesses in humans caused by the presence of a colony-forming illness-causing  E. coli  immunogen in meat by inhibiting the ability of the  E. coli  immunogen to adhere to the rumen or intestinal tracts of food animals to reduce the ability of the  E. coli  immunogen to multiply in the rumen or intestinal tracts of the food animals, said method comprising: 
 A. Inoculating female chickens, in or about to reach their egg laying age, with the illness-causing  E. coli  immunogen;    B. Allowing a period of time sufficient to permit the production in the eggs of the chickens of antibody to the  E. coli  immunogen, said antibody in the eggs including IgY immunoglobulins in the yolks of the eggs and IgM and IgA immunoglobulins in the albumin of the eggs;    C. Harvesting the eggs laid by the chickens;    D. Separating the entire contents of said harvested eggs from the shells;    E. Drying said separated entire contents of said eggs to provide a dried egg antibody product;    F. Mixing the resulting dried egg antibody product substantially uniformly through an animal feed or water; and    G. Supplying the resulting mixed dried egg antibody product and animal feed or water to food animals whereby the IgY immunoglobulins bind to  E. coli  immunogen, said binding being assisted by the IgM and IgA immunoglobulins to inhibit adherence of the  E. coli  immunogen in the intestinal tract of the food animals thereby reducing or eliminating the incidence of food borne illnesses in humans caused by the presence of  E. coli  immunogen in meat.    
     
     
         4 . The method according to  claim 3  including: providing a dry feed carrier, said drying of the antibody-containing contents of said eggs is achieved by coating the dry feed carrier with said separated antibody-containing contents of said eggs.  
     
     
         5 . The method of  claim 4  wherein: providing a dry feed carrier material from a group of materials including soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.  
     
     
         6 . A method for reducing or eliminating the incidence of illnesses in humans caused by the presence of a colony-forming illness-causing  Listeria  immunogen in meat by inhibiting the ability of the  Listeria  immunogen to adhere to the rumen or intestinal tracts of food animals to reduce the ability of the  Listeria  immunogen to multiply in the rumen or intestinal tracts of the food animals, said method comprising: 
 A. Inoculating female chickens, in or about to reach their egg laying age, with the illness-causing  Listeria  immunogen;    B. Allowing a period of time sufficient to permit the production in the eggs of the chickens of antibody to the  Listeria  immunogen, said antibody in the eggs including IgY immunoglobulins in the yolks of the eggs and IgM and IgA immunoglobulins in the albumin of the eggs;    C. Harvesting the eggs laid by the chickens;    D. Separating the entire contents of said harvested eggs from the shells;    E. Drying said separated entire contents of said eggs to provide a dried egg antibody product;    F. Distributing the resulting dried egg antibody product substantially uniformly through an animal feed or water; and    G. Supplying the resulting mixed dried egg antibody product and animal feed or water to food animals whereby the IgY immunoglobulins bind to the  Listeria  immunogen, said binding being assisted by the IgM and IgA immunoglobulins to inhibit adherence of the  Listeria  immunogen in the intestinal tract of the food animals thereby reducing or eliminating the incidence of food borne illnesses in humans caused by the presence of  Listeria  immunogen in meat.    
     
     
         7 . The method according to  claim 6  including: providing a dry feed carrier, said drying of the antibody-containing contents of said eggs is achieved by coating the dry feed carrier with said separated antibody-containing contents of said eggs.  
     
     
         8 . The method of  claim 7  wherein: providing a dry feed carrier material from a group of materials including soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.  
     
     
         9 . A method for reducing or eliminating the incidence of illnesses in humans caused by the presence of a colony-forming illness-causing  Salmonella  immunogen in meat by inhibiting the ability of the  Salmonella  immunogen to adhere to the rumen or intestinal tracts of food animals to reduce the ability of the  Salmonella  immunogen to multiply in the rumen or intestinal tracts of the food animals, said method comprising: 
 A. Inoculating female chickens, in or about to reach their egg laying age, with the illness-causing  Salmonella  immunogen;    B. Allowing a period of time sufficient to permit the production in the eggs of the chickens of antibody to the  Salmonella  immunogen, said antibody in the eggs including IgY immunoglobulins in the yolks of the eggs and IgM and IgA immunoglobulins in the albumin of the eggs;    C. Harvesting the eggs laid by the chickens;    D. Separating the entire contents of said harvested eggs from the shells;    E. Drying said separated entire contents of said eggs to provide a dried egg antibody product;    F. Mixing the resulting dried egg antibody product substantially uniformly through an animal feed or water; and    G. Supplying the resulting mixed dried egg antibody product and animal feed or water to food animals whereby the IgY immunoglobulins bind to the  Salmonella  immunogen, said binding being assisted by the IgM and IgA immunoglobulins to inhibit adherence of the  Salmonella  immunogen in the intestinal tract of the food animals thereby reducing or eliminating the incidence of food borne illnesses in humans caused by the presence of  Salmonella  immunogen in meat.    
     
     
         10 . The method according to  claim 9  including: providing a dry feed carrier, said drying of the antibody-containing contents of said eggs is achieved by coating the dry feed carrier with said separated antibody-containing contents of said eggs.  
     
     
         11 . The method of  claim 10  wherein: providing a dry feed carrier material from a group of materials including soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.  
     
     
         12 . A method for reducing or eliminating the incidence of illnesses in humans caused by the presence of a colony-forming illness-causing  Campylobacter  immunogen in meat by inhibiting the ability of the  Campylobacter  immunogen to adhere to the rumen or intestinal tracts of food animals to reduce the ability of the  Campylobacter  immunogen to multiply in the rumen or intestinal tracts of the food animals, said method comprising: 
 A. Inoculating female chickens, in or about to reach their egg laying age, with the illness-causing  Campylobacter  immunogen;    B. Allowing a period of time sufficient to permit the production in the eggs of the chickens of antibody to the  Campylobacter  immunogen, said antibody in the eggs including IgY immunoglobulins in the yolks of the eggs and IgM and IgA immunoglobulins in the albumin of the eggs;    C. Harvesting the eggs laid by the chickens;    D. Separating the entire contents of said harvested eggs from the shells;    E. Drying said separated entire contents of said eggs to provide a dried egg antibody product;    F. Mixing the resulting dried egg antibody product substantially uniformly through an animal feed or water; and    G. Supplying the resulting mixed dried egg antibody product and animal feed or water to food animals whereby the IgY immunoglobulins bind to the  Campylobacter  immunogen, said binding being assisted by the IgM and IgA immunoglobulins to inhibit adherence of the  Campylobacter  immunogen in the intestinal tract of the food animals thereby reducing or eliminating the incidence of food borne illnesses in humans caused by the presence of  Campylobacter  immunogen in meat.    
     
     
         13 . The method according to  claim 12  including: providing a dry feed carrier, said drying of the antibody-containing contents of said eggs is achieved by coating the dry feed carrier with said separated antibody-containing contents of said eggs.  
     
     
         14 . The method of  claim 13  wherein: providing a dry feed carrier material from a group of materials including soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.  
     
     
         15 . A method for reducing or eliminating the incidence of illnesses in humans caused by the presence of a targeted colony-forming illness-causing immunogen from the class consisting of  E. coli, Listeria, Salmonella  and  Campylobacter  in meat by inhibiting the ability of the immunogen to adhere to the rumen or intestinal tracts of food animals to reduce the ability of the immunogen to multiply in the rumen or intestinal tracts of food animals, said method comprising: 
 A. Inoculating female chickens, in or about to reach their egg laying age, with a particular targeted colony-forming illnesses-causing immunogen from the class consisting of  E. coli, Listeria, Salmonella  and  Campylobacter;      B. Allowing a period of time sufficient to permit the production in the eggs of the chickens of antibody to the targeted immunogen, said antibody in the eggs including IgY immunoglobulins in the yolks of the eggs and IgM and IgA immunoglobulins in the albumin of the eggs;    C. Harvesting the eggs laid by the chickens;    D. Separating the entire contents of said harvested eggs from the shells;    E. Providing a dry feed carrier material;    F. Coating said dry feed carrier material with the separated entire contents of said harvested eggs;    G. Mixing said carrier material coated with the separated entire contents of said harvested eggs substantially uniformly in animal feed; and    H. Supplying the resulting mixed dry carrier material coated with the separated entire contents of said harvested eggs and animal feed to food animals whereby the IgY immunoglobulins bind to the targeted colony-forming illness-causing immunogen, said binding being assisted by the IgM and IgA immunoglobulins to inhibit adherence of the targeted colony-forming illness-causing immunogen in the rumen or intestinal tracts of the food animals thereby reducing or eliminating the incidence of food borne illnesses in humans caused by the presence of the targeted colony-forming illness-causing immunogen in meat.    
     
     
         16 . The method of  claim 15  wherein: providing a dry feed carrier material from a group of materials including soybean hulls, rice hulls, corn, cottonseed hulls, distilled dried grains and beet pulp.