Mammalian secreted proteins

Abstract

The present invention provides to polynucleotides and secreted proteins encoded by the polynucleotides. The proteins include a variety of fusion proteins, including fusions comprising a signal peptide selected from the group consisting of signal peptides shown in SEQ ID NO:M, wherein M is an even integer from 2 to 328, operably linked to a second polypeptide. The invention further provides therapeutic and diagnostic methods utilizing the polynucleotides, polypeptides, and antagonists of the polypeptides.

1 . An isolated polypeptide comprising fifteen contiguous amino acid residues of a polypeptide as shown in SEQ ID NO:M, wherein M is an even integer from 2 to 328.  
     
     
         2 . The isolated polypeptide of  claim 1  which is from 15 to 723 amino acid residues in length.  
     
     
         3 . The isolated polypeptide of  claim 2 , wherein said at least fifteen contiguous amino acid residues of SEQ ID NO:M are operably linked via a peptide bond or polypeptide linker to a second polypeptide selected from the group consisting of maltose binding protein, an immunoglobulin constant region, a polyhistidine tag, and a peptide as shown in SEQ ID NO:329.  
     
     
         4 . The isolated polypeptide of  claim 1  comprising at least 30 contiguous residues of SEQ ID NO:M.  
     
     
         5 . The isolated polypeptide of  claim 1  comprising at least 47 contiguous residues of SEQ ID NO:M.  
     
     
         6 . The isolated polypeptide of  claim 1  wherein the polypeptide comprises the mature protein encoded by SEQ ID NO:N, wherein N is an odd integer from 1 to 327.  
     
     
         7 . The isolated polypeptide of  claim 1  wherein the polypeptide comprises the full-length polypeptide encoded by SEQ ID NO:N, wherein N is an odd integer from 1 to 327.  
     
     
         8 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment encoding the polypeptide of  claim 1;  and    a transcription terminator.    
     
     
         9 . A cultured cell comprising the expression vector of  claim 8 .  
     
     
         10 . A method of producing a polypeptide comprising culturing the cell of  claim 9  under conditions whereby said sequence of nucleotides is expressed, and recovering said polypeptide.  
     
     
         11 . A polypeptide produced by the method of  claim 10 .  
     
     
         12 . An isolated polynucleotide encoding a fusion protein, said protein comprising a secretory peptide selected from the group consisting of secretory peptides shown in Table 1, operably linked to a second polypeptide.  
     
     
         13 . An expression vector comprising the following operably linked elements: 
 a transcription promoter;    a DNA segment encoding fusion protein, said protein comprising a secretory peptide selected from the group consisting of secretory peptides shown in Table 1, wherein M is an even integer from 2 to 328, operably linked to a second polypeptide; and    a transcription terminator.    
     
     
         14 . A cultured cell comprising the expression vector of  claim 13 , wherein the cell expresses the DNA segment and produces the encoded fusion protein.  
     
     
         15 . A method of producing a protein comprising culturing the cell of  claim 14  under conditions whereby said DNA segment is expressed, and recovering said second polypeptide.  
     
     
         16 . An antibody that specifically binds to a protein selected from of the group consisting of SEQ ID NO:M, wherein M is an even integer from 2 to 328.  
     
     
         17 . An isolated polypeptide comprising fourteen contiguous amino acid residues of a polypeptide as shown in SEQ ID NO:M, wherein M is an even integer from 2 to 328.  
     
     
         18 . A method of detecting protein secretion from a cell or tissue comprising detecting a mature MSP selected from the group consisting of SEQ ID NO:M, wherein M is an even integer from 2 to 328 in conditioned media or membrane extracts.