Method of immobilizing cells on solid-phase surface

Abstract

Aspects of the invention can provide a method of immobilizing a chemical compound having the affinity for the cell membrane on the solid-phase surface in a desired pattern. The method of immobilizing a cell in a desired pattern on a solid-phase surface by use of a first chemical compound having an affinity for the cell and can include a step of immobilizing a second chemical compound, which is more easily immobilized on the solid-phase surface than the first chemical compound dose and has a molecular binding site that can bind to the first chemical compound, on the solid-phase surface according to the pattern.

1 . A method of immobilizing a cell in a desired pattern on a solid-phase surface by use of a first chemical compound having an affinity for the cell, comprising: 
 immobilizing a second chemical compound on the solid-phase surface according to the pattern, the second chemical compound being more easily immobilized on the solid-phase surface than the first chemical compound and having a molecular binding site that binds to the first chemical compound.    
     
     
         2 . The method of immobilizing a cell according to  claim 1 , the second chemical compound being a compound having the molecular binding site that binds to the first chemical compound and a substrate binding site that forms a self assembled monolayer film on a solid-phase substrate surface.  
     
     
         3 . The method of immobilizing a cell according to  claim 1 , the second chemical compound being a compound having the molecular binding site that binds to the first chemical compound and a polymer molecule part that is physically adsorbed to a solid-phase substrate surface and forms an insoluble thin film.  
     
     
         4 . The method of immobilizing a cell according to  claim 1 , the molecular binding site being an amino group.  
     
     
         5 . The method of immobilizing a cell according to  claim 1 , a droplet containing the second chemical compound being discharged on the solid-phase surface according to the pattern in order to immobilize the second chemical compound.  
     
     
         6 . The method of immobilizing a cell according to  claim 1 , further comprising: 
 immobilizing the second chemical compound on overall the solid-phase surface; and    inactivating the molecular binding site of the second chemical compound other than the molecular binding site in the pattern.    
     
     
         7 . The method of immobilizing a cell according to  claim 6 , the molecular binding site of the second chemical compound being inactivated by irradiating the second chemical compound with a radiant energy ray.  
     
     
         8 . The method of immobilizing a cell according to  claim 1 , further comprising: 
 forming a cell adhesion preventing film in an area other than the pattern after the second chemical compound is immobilized.    
     
     
         9 . The method of immobilizing a cell according to  claim 1 , further comprising: 
 forming a cell adhesion preventing film in an area other than the pattern; and    immobilizing the second chemical compound according to the pattern.    
     
     
         10 . The method of immobilizing a cell according to  claim 9 , the cell adhesion preventing film being formed by discharging a droplet containing a cell adhesion preventing compound in the area other than the pattern.  
     
     
         11 . The method of immobilizing a cell according to  claim 9 , further comprising: 
 forming the cell adhesion preventing film over all the solid-phase surface; and    removing a cell adhesion preventing compound according to the pattern.    
     
     
         12 . The method of immobilizing a cell according to  claim 11 , the cell adhesion preventing film being removed by irradiating the cell adhesion preventing film with a radiant energy ray.  
     
     
         13 . The method of immobilizing a cell according to  claim 8 , the cell adhesion preventing film including polyethylene glycol, saccharide, polyester and polyisocyanate.  
     
     
         14 . The method of immobilizing a cell according to  claim 1 , further comprising: 
 immobilizing the second chemical compound having the binding site to which a protecting group is bound on overall the solid-phase surface; and    detaching the protecting group according to the pattern.    
     
     
         15 . The method of immobilizing a cell according to  claim 14 , the protecting group being detached by discharging a droplet of the second chemical compound containing a protection removal inducing compound according to the pattern.  
     
     
         16 . The method of immobilizing a cell according to  claim 14 , further comprising: 
 removing the second chemical compound by irradiating an area other than the pattern with a radiant energy ray; and    reacting the second chemical compound left in the pattern with a protection removal inducing compound.    
     
     
         17 . The method of immobilizing a cell according to  claim 7 , the radiant energy ray being an electromagnetic wave, such as at least one of ultraviolet rays, electron ray and ion beam.  
     
     
         18 . A cell array in which the cell is immobilized by the method of immobilizing a cell according to  claim 1.