Polyhydroxyalkanoate-containing structure and manufacturing method thereof

Abstract

A method for manufacturing polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with polyhydroxyalkanoate, the method comprises the steps of immobilizing a polyhydroxyalkanoate synthase on the base material surface, synthesizing, on the base material surface, polyhydroxyalkanoate using a 3-hydroxyacyl coenzyme A to become the substrate of the synthase and the synthase and coating at least a part of the base material surface with the synthesized polyhydroxyalkanoate, wherein the synthase contains an amino acid sequence capable of binding to the base material. A polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with a polyhydroxyalkanoate, comprises the base material, a polyhydroxyalkanoate synthase immobilized on the base material surface, and the polyhydroxyalkanoate with which at least a part of the base material surface is coated, wherein the synthase contains an amino acid sequence capable of binding to the base material.

1 - 18 . (canceled)  
     
     
         19 . A polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with a polyhydroxyalkanoate, comprising: 
 the base material, a polyhydroxyalkanoate synthase immobilized on the base material surface, and the polyhydroxyalkanoate with which at least a part of said base material surface is coated, wherein said synthase contains an amino acid sequence capable of binding to said base material.    
     
     
         20 . The polyhydroxyalkanoate-containing structure according to  claim 19 , wherein 
 said polyhydroxyalkanoate is comprised of at least one selected from the group consisting of monomer units expressed by Formulas [1] to [10]:                          (wherein symbol “a” represents an integer, and the combination of R1 and “a” is selected from the group consisting of a combination of a hydrogen atom and any one integer selected from the group consisting of 0 to 10;    a combination of a halogen atom and any one integer selected from the group consisting of 1 to 10;    a combination of a chromophoric group and any one integer selected from the group consisting of 1 to 10;    a combination of a carboxyl group or a salt thereof and any one integer selected from the group consisting of 1 to 10; and    a combination of                          and any one integer selected from the group consisting of 1 to 7),                          (wherein b represents any one integer selected from the group consisting of 0 to 7, and R2 is selected from the group consisting of a hydrogen atom (H), a halogen atom, —CN, —NO 2 , —CF 3 , —C 2 F 5  and —C 3 F 7 ),                          (wherein c represents any one integer selected from the group consisting of 1 to 8, and R3 is selected from the group consisting of a hydrogen atom (H), a halogen atom, —CN, —NO 2 , —CF 3 , —C2F 5  and —C 3 F 7 ),                          (wherein d represents any one integer selected from the group consisting of 0 to 7, and R4 is selected from the group consisting of a hydrogen atom (H), a halogen atom, —CN, —NO 2 , —CF 3 , —C 2 F 5  and —C 3 F 7 ),                          (wherein e represents any one integer selected from the group consisting of 1 to 8, and R5 is selected from the group consisting of a hydrogen atom (H), a halogen atom, —CN, —NO 2 , —CF 3 , —C 2 F 5 , C 3 F 7 , —CH 3 , —C 2 H 5  and —C 3 H 7 ),                          (wherein f represents any one integer selected from the group consisting of 0 to 7),                          (wherein g represents any one integer selected from the group consisting of 1 to 8),                          (wherein h represents any one integer selected from the group consisting of 1 to 7, and R6 is selected from the group consisting of a hydrogen atom (H), a halogen atom, —CN, —NO 2 , —COOR′, —SO 2 R″, —CH 3 , —C 2 H 5 , —C 3 H 7 , —CH(CH 3 ) 2  and —C(CH 3 ) 3 , wherein R′ is selected from the group consisting of a hydrogen atom (H), Na, K, —CH 3  and —C 2 H 5 , and R″ is selected from the group consisting of —OH, ONa, —OK, a halogen atom, —OCH 3  and —OC 2 H 5 ),                          (wherein i represents any one integer selected from the group consisting of 1 to 7, and R7 is selected from the group consisting of a hydrogen atom (H), a halogen atom, —CN, —NO 2 , —COOR′ and —SO 2 R″, wherein R′ is selected from the group consisting of a hydrogen atom (H), Na, K, —CH 3  and —C 2 H 5 , and R″ is selected from the group consisting of —OH, —ONa, —OK, a halogen atom, —OCH 3  and —OC 2 H 5 ), and                          (wherein j represents any one integer selected from the group consisting of 1 to 9).    
     
     
         21 . The polyhydroxyalkanoate-containing structure according to  claim 20 , wherein 
 said polyhydroxyalkanoate has a hydrophilic functional group.    
     
     
         22 . The polyhydroxyalkanoate-containing structure according to  claim 21 , wherein 
 said polyhydroxyalkanoate has an anionic functional group.    
     
     
         23 . The polyhydroxyalkanoate-containing structure according to  claim 22 , wherein 
 said polyhydroxyalkanoate has a carboxyl group.    
     
     
         24 . The polyhydroxyalkanoate-containing structure according to  claim 23 , wherein 
 said polyhydroxyalkanoate having a carboxyl group is comprised of at least one selected from the group consisting of monomer units indicated by Formula [11],                          wherein k is an integer of 1 to 10.    
     
     
         25 . The polyhydroxyalkanoate-containing structure according to  claim 19 , wherein 
 the monomer unit composition of said polyhydroxyalkanoate is made to change in the lamination direction of said polyhydroxyalkanoate-containing structure.    
     
     
         26 . The polyhydroxyalkanoate-containing structure according to  claim 19 , wherein 
 at least a part of said polyhydroxyalkanoate is chemically modified.    
     
     
         27 . The polyhydroxyalkanoate-containing structure according to  claim 26 , wherein 
 said chemically modified polyhydroxyalkanoate is polyhydroxyalkanoate having at least a graft chain.    
     
     
         28 . The polyhydroxyalkanoate-containing structure according to  claim 27 , wherein 
 said graft chain is a graft chain made by chemically modifying polyhydroxyalkanoate containing at least a monomer unit having an epoxy group.    
     
     
         29 . The polyhydroxyalkanoate-containing structure according to  claim 27 , wherein 
 said graft chain is a graft chain of a compound having an amino group.    
     
     
         30 . The polyhydroxyalkanoate-containing structure according to  claim 29 , wherein 
 said compound having an amino group is a terminal amino-modified compound.    
     
     
         31 . The polyhydroxyalkanoate-containing structure according to  claim 30 , wherein 
 said terminal amino-modified compound is at least one selected from the group consisting of a polyvinylamine, polyethyleneimine and a terminal amino-modified polysiloxane.    
     
     
         32 . The polyhydroxyalkanoate-containing structure according to  claim 26 , wherein 
 at least a part of said polyhydroxyalkanoate is a crosslinked polyhydroxyalkanoate.    
     
     
         33 . The polyhydroxyalkanoate-containing structure according to  claim 32 , wherein 
 said crosslinked polyhydroxyalkanoate is polyhydroxyalkanoate made by crosslinking polyhydroxyalkanoate containing at least a monomer unit having an epoxy group.    
     
     
         34 . The polyhydroxyalkanoate-containing structure according to  claim 32 , wherein 
 said crosslinked polyhydroxyalkanoate is polyhydroxyalkanoate crosslinked by at least one selected from the group consisting of a diamine compound, succinic anhydride, 2-methyl-4-methylimidazole and electron ray irradiation.    
     
     
         35 . The polyhydroxyalkanoate-containing structure according to  claim 34 , wherein 
 said diamine compound is hexamethyldiamine.    
     
     
         36 - 45 . (canceled)  
     
     
         46 . The polyhydroxyalkanoate-containing structure according to  claim 19 , wherein 
 the base material is copper phthalocyanine and the amino acid sequence capable of binding to said base material is all or a part of at least one amino acid sequence selected from the group consisting of followings:                              Lys-Tyr-Asp-Ser-Arg-His-Leu-His-   (SEQ ID NO: 24)         Thr-His-Ser-His           Pro-Asn-Arg-Leu-Gly-Arg-Arg-Pro-   (SEQ ID NO: 25)     Val-Arg-Trp-Glu           Lys-Cys-Cys-Tyr-Tyr-Asp-His-Ser-   (SEQ ID NO: 26)     His-Ala-Leu-Ser           Glu-Tyr-Leu-Ser-Ala-Ile-Val-Ala-   (SEQ ID NO: 27)     Gly-Pro-Trp-Pro           Lys-Leu-Trp-Ile-Leu-Glu-Pro-Thr-   (SEQ ID NO: 28)     Val-Thr-Pro-Thr           Gln-Ser-Asn-Leu-Lys-Val-Ile-Pro-   (SEQ ID NO: 29)     Ser-Trp-Trp-Phe           Trp-Ile-Pro-Pro-Gln-Trp-Ser-Arg-   (SEQ ID NO: 30)     Leu-Ile-Glu-Pro           Asp-His-Pro-Gln-Ala-Lys-Pro-Asn-   (SEQ ID NO: 31)     Trp-Tyr-Gly-Val           Gly-Leu-Pro-Pro-Tyr-Ser-Pro-His-   (SEQ ID NO: 32)     Arg-Leu-Ala-Gln           Lys-Leu-Thr-Thr-Gln-Tyr-Met-Ala-   (SEQ ID NO: 33)     Arg-Ser-Ser-Ser           Lys-Val-Trp-Met-Leu-Pro-Pro-Leu-   (SEQ ID NO: 34)     Pro-Gln-Ala-Thr           Asn-Val-Thr-Ser-Thr-Ala-Phe-Ile-   (SEQ ID NO: 35)     Asp-Thr-Pro-Trp           Arg-Leu-Asn-Leu-Asp-Ile-Ile-Ala-   (SEQ ID NO: 36)     Val-Thr-Ser-Val           Thr-Leu-Pro-Ser-Pro-Leu-Ala-Leu-   (SEQ ID NO: 37)     Leu-Thr-Val-His           Thr-Asn-Arg-His-Asn-Pro-His-His-   (SEQ ID NO: 38)     Leu-His-His-Val                                                                    
     
     
         47 . The polyhydroxyalkanoate-containing structure according to  claim 46 , wherein 
 the base material is copper phthalocyanine and the amino acid sequence capable of binding to said base material is all or a part of Lys-Tyr-Asp-Ser-Arg-His-Leu-His-Thr-His-Ser-His (SEQ ID NO:24).    
     
     
         48 . The polyhydroxyalkanoate-containing structure according to  claim 46 , wherein 
 the base material is copper phthalocyanine and the amino acid sequence capable of binding to said base material is all or a part of Pro-Asn-Arg-Leu-Gly-Arg-Arg-Pro-Val-Arg-Trp-Glu (SEQ ID NO:25).    
     
     
         49 . The polyhydroxyalkanoate-containing structure according to  claim 19 , wherein 
 the base material is Carbon Black and the amino acid sequence capable of binding to said base material is all or a part of at least one amino acid sequence selected from the group consisting of following:                              Trp-Pro-His-Ala-Trp-Lys-Val-Trp-   (SEQ ID NO: 39)         Trp-Pro-Ala-Ser           Asn-Trp-Trp-Trp-Pro-Pro-Tyr-Ile-   (SEQ ID NO: 40)     Arg-His-Gln-Pro           Trp-His-Trp-Ser-Trp-Thr-Pro-Trp-   (SEQ ID NO: 41)     Pro-Ser-His-His           Trp-Pro-Trp-Ala-Trp-His-Pro-Ser-   (SEQ ID NO: 42)     Arg-Asp-Val-Tyr           Trp-His-Gly-Tyr-Trp-Tyr-Ser-Asn-   (SEQ ID NO: 43)     Leu-Asn-Thr-Thr           Trp-Trp-Thr-Pro-Trp-Met-Ser-His-   (SEQ ID NO: 44)     Ala-Tyr-Pro-Val           Trp-Pro-Asn-Pro-Tyr-Trp-Gly-Trp-   (SEQ ID NO: 45)     Phe-Ala-Ala-Val           Thr-Ser-Trp-His-Thr-Trp-Trp-Trp-   (SEQ ID NO: 46)     Arg-Gln-Pro-Pro           Asn-Ala-Trp-His-Lys-Tyr-Trp-Trp-   (SEQ ID NO: 47)     Pro-Ile-Thr-Lys           His-Pro-Asn-Asn-Asp-Trp-Ser-Lys-   (SEQ ID NO: 48)     Ala-Pro-Gln-Phe           Trp-Trp-Thr-Pro-Gln-Pro-Trp-Trp-   (SEQ ID NO: 49)     Ser-Phe-Pro-Ile           Trp-Pro-His-Thr-Ser-Trp-Trp-Gln-   (SEQ ID NO: 50)     Thr-Pro-Leu-Thr           Trp-His-Val-Asn-Trp-Asp-Pro-Met-   (SEQ ID NO: 51)     Ala-Trp-Tyr-Arg           Ser-Trp-Pro-Trp-Trp-Thr-Ala-Tyr-   (SEQ ID NO: 52)     Arg-Val-His-Ser           Trp-His-Ser-Asn-Trp-Tyr-Gln-Ser-   (SEQ ID NO: 53)     Ile-Pro-Gln-Val           Gly-Tyr-Trp-Pro-Trp-Lys-Phe-Glu-   (SEQ ID NO: 54)     His-Ala-Thr-Val           Ala-Trp-Trp-Pro-Thr-Thr-Phe-Pro-   (SEQ ID NO: 55)     Pro-Tyr-Tyr-Tyr           Asn-Pro-Trp-Trp-Ser-His-Tyr-Tyr-   (SEQ ID NO: 56)     Pro-Arg-Ser-Val           Trp-Pro-His-Asn-Tyr-Pro-Leu-Asn-   (SEQ ID NO: 57)     His-Ser-Asn-Pro           Thr-Trp-Ala-His-Pro-Leu-Glu-Ser-   (SEQ ID NO: 58)     Asp-Tyr-Leu-Arg           His-Thr-Tyr-Tyr-His-Asp-Gly-Trp-   (SEQ ID NO: 59)     Arg-Leu-Ala-Pro           Thr-Phe-Val-Gln-Thr-Pro-Leu-Ser-   (SEQ ID NO: 60)     His-Leu-Ile-Ala           Arg-Val-Pro-Pro-Ser-Lys-Leu-Thr-   (SEQ ID NO: 61)     Arg-Pro-Pro-Phe           His-Ser-Ile-Tyr-Ser-Val-Thr-Pro-   (SEQ ID NO: 62)     Ser-Thr-Ala-Ser           Leu-Asn-Thr-Gln-Asn-His-Ala-Pro-   (SEQ ID NO: 63)     Leu-Pro-Ser-Ile                                                                                                  
     
     
         50 . The polyhydroxyalkanoate-containing structure according to  claim 49 , wherein 
 the base material is Carbon Black and the amino acid sequence capable of binding to said base material is all or a part of Trp-Pro-His-Ala-Trp-Lys-Val-Trp-Trp-Pro-Ala-Ser (SEQ ID NO:39).    
     
     
         51 . The polyhydroxyalkanoate-containing structure according to  claim 49 , wherein 
 the base material is Carbon Black and the amino acid sequence capable of binding to said base material is all or a part of Asn-Trp-Trp-Trp-Pro-Pro-Tyr-Ile-Arg-His-Gln-Pro (SEQ ID NO:40).    
     
     
         52 . The polyhydroxyalkanoate-containing structure according to  claim 19 , wherein 
 the base material is titanium oxide and the amino acid sequence capable of binding to said base material is all or a part of at least one amino acid sequence selected from the group consisting of followings:                              His-Ala-Thr-Gly-Thr-His-Gly-Leu-   (SEQ ID NO: 150)         Ser-Leu-Ser-His           Thr-Leu-Pro-Ser-Pro-Leu-Ala-Leu-   (SEQ ID NO: 151)     Leu-Thr-Val-His           Leu-Ser-Thr-His-Tyr-Val-Asn-Arg-   (SEQ ID NO: 152)     Ser-His-Ile-Thr           Ala-Tyr-His-Ile-Asn-Gln-Leu-Gly-   (SEQ ID NO: 153)     Ala-Pro-Pro-Ala           Leu-His-Leu-Thr-Pro-His-Pro-Gly-   (SEQ ID NO: 154)     Asp-Thr-Leu-Thr           Gln-Asp-Val-His-Leu-Thr-Gln-Gln-   (SEQ ID NO: 155)     Ser-Arg-Tyr-Thr           Leu-Glu-Ile-Pro-Ser-Asn-Gly-Leu-   (SEQ ID NO: 156)     Asn-His-Lys-Ile           Leu-Glu-Ile-Pro-Ser-Asn-Gly-Leu-   (SEQ ID NO: 157)     Asn-His-Asn-Ile                                               
     
     
         53 . The polyhydroxyalkanoate-containing structure according to  claim 52 , wherein 
 the base material is titanium oxide and the amino acid sequence capable of binding to said base material is all or a part of His-Ala-Thr-Gly-Thr-His-Gly-Leu-Ser-Leu-Ser-His (SEQ ID NO:150).    
     
     
         54 . The polyhydroxyalkanoate-containing structure according to  claim 52 , wherein 
 the base material is titanium oxide and the amino acid sequence capable of binding to said base material is all or a part of Thr-Leu-Pro-Ser-Pro-Leu-Ala-Leu-Leu-Thr-Val-His (SEQ ID NO:151).    
     
     
         55 . The polyhydroxyalkanoate-containing structure according to  claim 19 , wherein 
 the base material is a silicon board and the amino acid sequence capable of binding to said base material is Asp-Ser-His-Phe-Thr-Ile-Asn (SEQ ID NO:21).    
     
     
         56 - 65 . (canceled)  
     
     
         66 . A static charge image developing toner containing a coloring agent obtained by coating at least a part of a surface of a coloring material with polyhydroxyalkanoate, wherein 
 the structure according to  claim 19  in which the base material of the structure is a coloring material is at least a part of a constitution of the toner.    
     
     
         67 . A static charge image developing toner containing a coloring agent obtained by coating at least a part of a surface of copper phthalocyanine with polyhydroxyalkanoate, wherein 
 the structure according to  claim 46  is at least a part of constitution of the toner.    
     
     
         68 . A static charge image developing toner containing a coloring agent obtained by coating at least a part of a surface of Carbon Black with polyhydroxyalkanoate, wherein 
 the structure according to  claim 49  is at least a part of the constitution of the toner.    
     
     
         69 . A static charge image developing toner containing a coloring agent obtained by coating at least a part of a surface of titanium oxide with polyhydroxyalkanoate, wherein 
 the structure according to  claim 52  is at least a part of the constitution of the toner.    
     
     
         70 . A coloring composition for a color filter containing a coloring agent obtained by coating at least a part of a surface of a coloring material with polyhydroxyalkanoate, wherein 
 the structure according to  claim 19  in which the base material of the structure is a coloring material is at least a part of the constitution of the toner.    
     
     
         71 . A coloring composition for a color filter containing a coloring agent obtained by coating at least a part of a surface of copper phthalocyanine with polyhydroxyalkanoate, wherein 
 the structure according to  claim 46  is at least a part of the constitution of the composition.    
     
     
         72 . A coloring composition for a color filter containing a coloring agent obtained by coating at least a part of a surface of Carbon Black with polyhydroxyalkanoate, wherein 
 any one of the structures according to  claim 49  is at least a part of the constitution of the composition.    
     
     
         73 . A coloring composition for a color filter containing a coloring agent obtained by coating at least a part of a surface of titanium oxide with polyhydroxyalkanoate, wherein 
 the structure according to  claim 52  is at least a part of the constitution of the composition.    
     
     
         74 . An electrophoretic particle containing a coloring agent obtained by coating at least a part of a surface of a coloring material with polyhydroxyalkanoate, wherein 
 the structure according to  claim 19  in which the base material of the structure is a coloring material is at least a part of the constitution of the particle.    
     
     
         75 . An electrophoretic particle containing a coloring agent obtained by coating at least a part of a surface of copper phthalocyanine with polyhydroxyalkanoate, wherein 
 the structure according to  claim 46  is at least a part of the constitution of the particle.    
     
     
         76 . An electrophoretic particle containing a coloring agent obtained by coating at least a part of a surface of Carbon Black with polyhydroxyalkanoate, wherein 
 the structure according to  claim 49  is at least a part of the constitution of the particle.    
     
     
         77 . An electrophoretic particle containing a coloring agent obtained by coating at least a part of a surface of titanium oxide with polyhydroxyalkanoate, wherein 
 the structure according to  claim 52  is at least a part of the constitution of the particle.    
     
     
         78 . A pigment ink containing a coloring agent obtained by coating at least a part of a surface of a coloring material with polyhydroxyalkanoate, wherein 
 any one of the structures according to  claim 19  in which the base material of the structure is a coloring material is at least a part of the constitution of the ink.    
     
     
         79 . A pigment ink containing a coloring agent obtained by coating at least a part of a surface of copper phthalocyanine with polyhydroxyalkanoate, wherein 
 the structure according to  claim 46  is at least a part of the constitution of the ink.    
     
     
         80 . A pigment ink containing a coloring agent obtained by coating at least a part of a surface of Carbon Black with polyhydroxyalkanoate, wherein 
 the structure according to  claim 49  is at least a part of the constitution of the ink.    
     
     
         81 . A pigment ink containing a coloring agent obtained by coating at least a part of a surface of titanium oxide with polyhydroxyalkanoate, wherein 
 the structure according to  claim 52  is at least a part of the constitution of the ink.