US2005208030A1PendingUtilityA1

Myocardial grafts and cellular compositions useful for same

66
Individually held — no corporate assignee on recordPriority: Nov 16, 1993Filed: Nov 16, 2004Published: Sep 22, 2005
Est. expiryNov 16, 2013(expired)· nominal 20-yr term from priority
Inventors:Loren J. Field
C12N 5/0658A01K 67/0271A01K 2217/05A61K 35/12A61K 35/34A61K 48/00C07K 14/4716C12N 5/0657C12N 2500/22C12N 2506/02C12N 2510/00C12N 2517/02
66
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Claims

Abstract

Described are preferred myocardial grafts of skeletal myoblasts or cardiomyocytes, and cellular compositions and methods useful in obtaining the grafts. The myocardial grafts are stable and can be used, for example, to deliver recombinant proteins directly to the heart.

Claims

exact text as granted — not AI-modified
1 . A myocardial graft in an animal, comprising: 
 a stable graft of skeletal myoblasts or cardiomyocytes incorporated in myocardial tissue of said animal.    
     
     
         2 . The myocardial graft of  claim 1  wherein said stable graft comprises cardiomyocytes.  
     
     
         3 . The myocardial graft of  claim 1  wherein said stable graft comprises skeletal myoblasts.  
     
     
         4 . The myocardial graft of  claim 1  which is non-tumorogenic.  
     
     
         5 . The myocardial graft of  claim 1  wherein said stable graft delivers recombinant molecules to the myocardial tissue.  
     
     
         6 . The myocardial graft of  claim 5  which is non-tumorogenic.  
     
     
         7 . A method for forming a stable myocardial graft in an animal, comprising: 
 introducing skeletal myoblasts or cardiomyocytes in myocardial tissue of the animal so as to form a stable myocardial graft.    
     
     
         8 . The method of  claim 7  wherein said introducing comprises injecting the skeletal myoblasts or cardiomyocytes into myocardial tissue of the animal.  
     
     
         9 . The method of  claim 7  in which skeletal myoblasts are introduced into the myocardial tissue.  
     
     
         10 . The method of  claim 7  in which cardiomyocytes are introduced into the myocardial tissue.  
     
     
         11 . The method of  claim 7  wherein the myocardial graft is non-tumorogenic.  
     
     
         12 . The method of  claim 11  wherein the myocardial graft delivers recombinant molecules to the myocardial tissue.  
     
     
         13 . A method for delivering recombinant molecules to myocardial tissue of an animal, comprising: 
 establishing a stable graft of skeletal myoblasts or cardiomyocytes incorporated in myocardial tissue of the animal, wherein the myoblasts or cardiomyocytes deliver recombinant molecules to the myocardial tissue.    
     
     
         14 . The method of  claim 13  wherein the stable graft comprises skeletal myoblasts.  
     
     
         15 . The method of  claim 13  wherein the stable graft comprises cardiomyocytes.  
     
     
         16 . The method of  claim 13  wherein the graft is non-tumorogenic.  
     
     
         17 . A cellular composition comprising a substantially homogeneous population of non-immortalized cardiomyocytes.  
     
     
         18 . A method of obtaining a substantially homogeneous population of cells, comprising: 
 transfecting embryonic stem cells to introduce a marker gene enabling selection of one cell lineage from other cell lineages resulting from the differentiation of the stem cells;    causing the stem cells to differentiate; and    selecting said one cell lineage based on said marker gene.    
     
     
         19 . The method of  claim 18 , comprising: 
 transfecting the stem cells to introduce (i) a first marker gene enabling selection of transfected stem cells from non-transfected stem cells and (ii) a second marker gene enabling selection of said one cell lineage from said other cell lineages;    selecting transfected stem cells based on the first marker gene;    causing the selected. stem cells to differentiate; and    selecting said one cell lineage based on said marker gene.    
     
     
         20 . The method of  claim 19  wherein said one cell lineage is cardiomyocytes.  
     
     
         21 . A non-human animal having a stable graft of skeletal myoblasts or cardiomyocytes incorporated in myocardial tissue of the animal.  
     
     
         22 . The animal of  claim 21  which is a mammal.  
     
     
         23 . The animal of  claim 22  wherein the graft is non-tumorogenic.  
     
     
         24 . The animal of  claim 23  wherein the graft includes cardiomyocytes.  
     
     
         25 . The animal of  claim 23  wherein the graft includes skeletal myoblasts.

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