Hybridization probe and target nucleic acid detecting kit, target nucleic acid detecting apparatus and target nucleic acid detecting method using the same

Abstract

The present invention relates to a hybridization probe having a rod-shaped body and nucleic acid which is bonded to the rod-shape material and specifically bonds to a target nucleic acid and also relates to a target nucleic acid detecting kit, a target nucleic acid detecting apparatus and a target nucleic acid detecting method using the same.

1 - 14 . (canceled)  
     
     
         15 . A target nucleic acid detecting apparatus for detecting a target nucleic acid, comprising: 
 a hybridization probe;    means for adding a sample to the hybridization probe; and    means for measuring changes in a wavelength by the colored interference light of the hybridization probe hybridized aligned in a film-like shape to the target nucleic acid;    wherein the hybridization probe comprises a rod-shaped body and a nucleic acid bonded to the rod-shaped body in a length of 810 nm or shorter and specifically bonds to a target nucleic acid and reflects the incident light as colored interference light when aligned in a film-like shape.    
     
     
         16 . A target nucleic acid detecting apparatus according to  claim 15 , wherein the hybridization probe is amphiphilic and the means for adding is an adding means in which the hybridization probe is added to an aqueous phase together with an oil phase so that the hybridization probe contacts the sample.  
     
     
         17 . A target nucleic acid detecting apparatus comprising: 
 a biosensor which comprises one of quartz oscillator and surface acoustic wave (SAW) element and a hybridization probe aligned to the one of quartz oscillator and surface acoustic wave (SAW) element in a film-like shape;    an oscillation circuit in which changes in mass or changes in viscoelasticity when a target nucleic acid is hybridized to the biosensor are oscillated as frequency; and    a frequency counter in which a frequency oscillated from the oscillation circuit is measured;    wherein the hybridization probe comprises a rod-shaped body and a nucleic acid bonded to the rod-shaped body in a length of 810 nm or shorter and specifically bonds to a target nucleic acid and reflects the incident light as colored interference light when aligned in a film-like shape and the hybridization probe is amphiphilic.    
     
     
         18 . The target nucleic acid detecting apparatus according to  claim 17 , wherein the hybridization probe is aligned in a monomolecular film-like shape to one of the quartz oscillator and the surface acoustic wave element.  
     
     
         19 . A target nucleic acid detecting apparatus according to  claim 17 , wherein the hybridization probe is aligned in a bimolecular film-like shape to one of the quartz oscillator and the surface acoustic wave element.  
     
     
         20 . A target nucleic acid detecting method apparatus for detecting a target nucleic acid, comprising: 
 a step for contacting a sample to a hybridization probe;    a step for measuring changes in a wavelength by the colored interference light of the hybridization probe hybridized to the target nucleic acid;    wherein the hybridization probe comprises a rod-shaped body and a nucleic acid bonded to the rod-shaped body in a length of 810 nm or shorter and specifically bonds to a target nucleic acid and reflects the incident light as colored interference light when aligned in a film-like shape and the hybridization probe is amphiphilic.