Plant protein disulfide isomerase

Abstract

This invention relates to an isolated nucleic acid fragment encoding a protein disulfide isomerase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the protein disulfide isomerase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the protein disulfide isomerase in a transformed host cell.

1 - 15 . (canceled)  
     
     
         16 . An isolated polynucleotide comprising: 
 (a) a nucleotide sequence encoding a polypeptide having disulfide isomerase activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:16 have at least 90% identity, or    (b) the complement of the nucleotide sequence, wherein the complement and the nucleotide sequence contain the same number of nucleotides and are 100% complementary.    
     
     
         17 . The polynucleotide of  claim 16  wherein the amino acid sequence identity is at least 95%.  
     
     
         18 . The polynucleotide of  claim 16  wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:16.  
     
     
         19 . The polynucleotide of  claim 16  wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:15.  
     
     
         20 . A chimeric gene comprising the polynucleotide of  claim 16  operably linked to at least one regulatory sequence.  
     
     
         21 . A cell comprising the polynucleotide of  claim 16 .  
     
     
         22 . The cell of  claim 21 , wherein the cell is selected from the group consisting of a yeast cell, a bacterial cell and a plant cell.  
     
     
         23 . A transgenic plant comprising the polynucleotide of  claim 16 .  
     
     
         24 . A virus comprising the polynucleotide of  claim 16 .  
     
     
         25 . A method for transforming a cell comprising introducing into a cell the polynucleotide of  claim 16 .  
     
     
         26 . A method for producing a transgenic plant comprising (a) transforming a plant cell with the polynucleotide of  claim 16  and (b) regenerating a plant from the transformed plant cell.  
     
     
         27 . A vector comprising the polynucleotide of  claim 16 .  
     
     
         28 . A seed comprising the chimeric gene of  claim 20 .  
     
     
         29 . A method for isolating a polypeptide encoded by the polynucleotide of  claim 16  comprising isolating the polypeptide from a cell transformed with said polynucleotide.  
     
     
         30 . A composition comprising an isolated polynucleotide comprising a nucleotide sequence encoding a first polypeptide of at least 100 amino acids that has at least 85% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of a polypeptide of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, and 20, or an isolated polynucleotide comprising the complement of the nucleotide sequence.