Nematode ATP synthase subunit E-like sequences

Abstract

Nucleic acid molecules from nematodes encoding ATP synthase subunit E polypeptides are described. ATP synthase subunit E-like polypeptide sequences are also provided, as are vectors, host cells, and recombinant methods for production of ATP synthase subunit E-like nucleotides and polypeptides. Also described are screening methods for identifying inhibitors and/or activators, as well as methods for antibody production.

1 - 42 . (canceled)  
     
     
         43 . A method comprising: 
 (a) providing a polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E, the polypeptide having ATP synthase subunit E activity;    (b) contacting the polypeptide with a test compound; and    (c) measuring the binding of the test compound to the polypeptide.    
     
     
         44 . The method of  claim 43 , further comprising: 
 (d) measuring the ATP synthase subunit E activity of the polypeptide in the presence of the test compound.    
     
     
         45 . The method of  claim 43 , further comprising: 
 (d) providing a second polypeptide, wherein the second polypeptide comprises the amino acid sequence of a mammalian or plant ATP synthase subunit E and has ATP synthase subunit E activity;    (e) contacting the second polypeptide with a test compound; and    (f) measuring the binding of the test compound to the second polypeptide.    
     
     
         46 . A method comprising: 
 (a) providing a polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E, the polypeptide having ATP synthase subunit E activity;    (b) contacting the polypeptide with a test compound; and    (c) measuring a ATP synthase subunit E activity of the polypeptide, wherein a change in ATP synthase subunit E activity of the polypeptide in the presence of the test compound relative to the ATP synthase subunit E activity of the polypeptide in the absence of the test compound is an indication that the test compound alters the ATP synthase subunit E activity of the polypeptide.    
     
     
         47 . The method of  claim 46 , further comprising: 
 (d) providing a second polypeptide, wherein the second polypeptide comprises the amino acid sequence of a mammalian or ATP synthase subunit E and has ATP synthase subunit E activity;    (e) contacting the second polypeptide with the test compound; and    (f) measuring a ATP synthase subunit E activity of the second polypeptide in the presence of the test compound.    
     
     
         48 . The method of  claim 43  or  46  wherein the polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E comprises an amino acid sequence that is at least 70% identical to the amino acid sequence of SEQ ID NO: 4.  
     
     
         49 . The method of  claim 48  wherein the polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E comprises an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO: 4.  
     
     
         50 . The method of  claim 48  wherein the polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E comprises an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 4.  
     
     
         51 . The method of  claim 48  wherein the polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E comprises an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 4.  
     
     
         52 . The method of  claim 43  or  46  wherein the polypeptide comprising the amino acid sequence of a nematode ATP synthase subunit E comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 4, 5, and 6.