Linoleate isomerase

Abstract

The present invention provides an isolated linoleate isomerase and its nucleic acid and amino acid sequence. The present invention also provides a method for producing CLA from an oil using an immobilized bacterial cell or an isolated linoleate isomerase.

1 - 27 . (canceled)  
     
     
         28 . An isolated antibody that selectively binds to an isolated linoleate isomerase of selected from the group consisting of  Lactobacillus, Clostridium, Propionibacterium, Butyrivibrio,  and  Eubacterium  linoleate isomerases.  
     
     
         29 - 44 . (canceled)  
     
     
         45 . An immobilized bacterial cell selected from the group consisting of immobilized  Lactobacillus, Clostridium, Propionibacterium, Butyrivibrio, Escherichia, Bacillus  and  Eubacterium  cells, wherein said cell comprises a genetic modification that increases the action of linoleate isomerase.  
     
     
         46 - 49 . (canceled)  
     
     
         50 . The immobilized bacterial cell of claim  47 , wherein said recombinant nucleic acid molecule comprises a nucleic acid sequence that hybridizes under stringent hybridization conditions to a nucleic acid molecule comprising the complement of a sequence selected from the group consisting of SEQ ID NO:4, SEQ ID NO:8, SEQ ED NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17 and SEQ ID NO:26.  
     
     
         51 . The immobilized bacterial cell of claim  47 , wherein said recombinant nucleic acid molecule comprises a nucleic acid sequence selected from the group consisting of SEQ ID NO:4, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17 and SEQ ID NO:26.  
     
     
         52 . The immobilized bacterial cell of claim  47 , wherein said recombinant nucleic acid molecule comprises nucleic acid sequence SEQ ID NO:17.  
     
     
         53 . The immobilized bacterial cell of claim  47 , wherein said recombinant nucleic acid molecule encodes a protein comprising an amino acid sequence with at least about 70% identity with the amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:5, SEQ ID NO:9, SEQ ID NO:11 and SEQ ID NO:18.  
     
     
         54 . The immobilized bacterial cell of claim  47 , wherein said recombinant nucleic acid molecule encodes a protein comprising amino acid sequence SEQ ID NO:18.  
     
     
         55 . (canceled)  
     
     
         56 . The immobilized bacterial cell of claim  47 , wherein said recombinant nucleic acid molecule encoding linoleate isomerase comprises a genetic modification which increases the action of said linoleate isomerase.  
     
     
         57 - 60 . (canceled)  
     
     
         61 . The immobilized bacterial cell of  claim 45 , wherein said cell has been immobilized by crosslinking with a bifunctional or multifunctional crosslinking agent.  
     
     
         62 . (canceled)  
     
     
         63 . Amended) A method for producing CLA, comprising contacting an oil, said oil comprising a compound selected from the group consisting of linoleic acid and linolenic acid, with the immobilized bacterial cell of  claim 45 .  
     
     
         64 - 78 . (canceled)  
     
     
         79 . An isolated nucleic acid molecule selected from the group consisting of: (a) a nucleic acid molecule comprising a nucleic acid sequence that encodes a protein having an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:5, SEQ ID NO:9, SEQ ID NO:11, and SEQ ID NO:18; (b) a nucleic acid molecule encoding a homologue of any of said amino acid sequences of (a), wherein said homologue comprises at least 8 contiguous amino acids having 100% identity with amino acids in said amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:5, SEQ ID NO:9, SEQ ID NO:11, and SEQ ID NO:18; (c) a nucleic acid molecule comprising a naturally occurring allelic variant of a nucleic acid molecule encoding any of said amino acid sequences of (a); and, (d) a nucleic acid molecule that is complementary to any of said nucleic acid molecules of (a), (b) or (c).  
     
     
         80 - 81 . (canceled)  
     
     
         82 . The nucleic acid molecule of  claim 79 , wherein said nucleic acid molecule comprises a nucleic acid sequence with at least about 70% identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO:4, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17 and SEQ ID NO:26.  
     
     
         83 . The nucleic acid molecule of  claim 79 , wherein said nucleic acid molecule comprises a nucleic acid sequence having at least 24 contiguous nucleotides having 100% identity with nucleic acid sequence SEQ ID NO:17.  
     
     
         84 . (canceled)  
     
     
         85 . The isolated nucleic acid molecule of  claim 79 , wherein said nucleic acid molecule encodes a protein comprising amino acid sequence SEQ ID NO:18.  
     
     
         86 . The isolated nucleic acid molecule of  claim 79 , wherein said nucleic acid molecule is selected from the group consisting of a nucleic acid molecule having a sequence selected from the group consisting of SEQ ID NO:4, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17 and SEQ ID NO:26.  
     
     
         87 . The isolated nucleic acid molecule of  claim 79 , wherein said nucleic acid molecule comprises nucleic acid sequence SEQ ID NO:17.  
     
     
         88 - 90 . (canceled)  
     
     
         91 . A recombinant molecule comprising an isolated nucleic acid molecule as set forth in  claim 79  operatively linked to a transcription control sequence.  
     
     
         92 . A recombinant virus comprising an isolated nucleic acid molecule as set forth in  claim 79 .  
     
     
         93 . A recombinant cell comprising an isolated nucleic acid molecule as set forth in  claim 79 , wherein said cell expresses said nucleic acid molecule.  
     
     
         94 - 96 . (canceled)  
     
     
         97 . Amended) A method to produce linoleate isomerase, comprising culturing a recombinant cell of  claim 93 .  
     
     
         98 - 99 . (canceled)