Microspore-specific promoter and method for producing hybrid plants

Abstract

The invention concerns a microspore-specific promoter and its use for obtaining plants with gametophytic male sterility with inducible fertility. It also concerns a method for obtaining hybrid plants.

1 - 6 . (canceled)  
     
     
         7 . Plants with gametophytic male sterility with inducible fertility, comprising a gene encoding a male-gamete-specific cytotoxic product.  
     
     
         8 . Method for producing plants with gametophytic male sterility with inducible fertility, comprising: 
 the insertion into plants of a line A of a gene whose-expression product is cytotoxic for the microspores, and    the production of plants which do not produce male gametes.    
     
     
         9 . Method for producing plants with gametophytic male sterility with inducible fertility according to  claim 8 , comprising the steps of: 
 a) transformation of plants of a line A with a vector according to claim  3  or  4 ,    b) induction of the fertility of the plants obtained in a) by inhibition of the cytotoxicity of the product,    c) self-fertilization of the fertile plants obtained in b),    d) selection of the plants which do not produce male gametes, derived from c),    e) multiplication of the plants obtained in d) by reproduction of steps b) and c).    
     
     
         10 . Method for producing plants according to  claim 8  or  9 , characterized in that, when the cytotoxic product is a subtilisin, the induction of the fertility consists in applying to the plant an insecticide molecule of the fluorophosphate family.  
     
     
         11 . Seeds derived from the hybrid plants obtained by crossing plants of line A, which have gametophytic male sterility with inducible fertility, according to  claim 7 , or as obtained by using the method according to one of  claims 8  to  10 , with plants of line B of agronomic value.  
     
     
         12 . (canceled)