Process for preparing clavam derivatives by using polypeptides having beta-lactam synthetase activity

Abstract

A process for the synthesis of clavam compounds, in particular clavulanic acid using β-lactam synthetase polypeptides and β-lactam synthetase polynucleotides.

1 - 32 . (canceled)  
     
     
         33 . A recombinant vector comprising a polynucleotide capable of producing a polypeptide having β-lactam synthetase activity and having at least 95% identity to the amino acid sequence of SEQ ID NO:2 over the entire length of SEQ ID NO:2.  
     
     
         34 . A recombinant vector according to  claim 33  comprising a polynucleotide comprising the polynucleotide sequence of SEQ ID NO:1.  
     
     
         35 . A host microorganism comprising a recombinant vector of claims  33 .  
     
     
         36 . A host microorganism according to  claim 35  which is selected from  Streptomyces , or  E. coli.