HADHs as modifiers of the p21 pathway and methods of use

Abstract

Human HADH genes are identified as modulators of the p21 pathway, and thus are therapeutic targets for disorders associated with defective p21 function. Methods for identifying modulators of p21, comprising screening for agents that modulate the activity of HADH are provided.

1 - 25 . (canceled)  
     
     
         26 . An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a polypeptide comprising amino acid residues 9 to 212 of SEQ ID NO:3.  
     
     
         27 . A vector comprising the nucleic acid molecule of  claim 26 .  
     
     
         28 . A host cell comprising the vector of  claim 27 .  
     
     
         29 . A process for producing a protein comprising a polypeptide comprising amino acid residues 9 to 212 of SEQ ID NO:3, said process comprising culturing the host cell of  claim 28  under conditions suitable for expression of said polypeptide and recovering said protein.  
     
     
         30 . An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a polypeptide that is at least 95% identical to amino acid residues 9 to 212 of SEQ ID NO:3.  
     
     
         31 . A vector comprising the nucleic acid molecule of  claim 30 .  
     
     
         32 . A host cell comprising the vector of  claim 31 .  
     
     
         33 . A process for producing a protein comprising a polypeptide comprising amino acid residues 9 to 212 of SEQ ID NO:3, said process comprising culturing the host cell of  claim 32  under conditions suitable for expression of said polypeptide and recovering said protein.  
     
     
         34 . An isolated nucleic acid molecule comprising a nucleic acid sequence that hybridizes under stringent conditions to a second nucleic acid molecule comprising a nucleic acid sequence encoding a polypeptide comprising amino acid residues 9 to 212 of SEQ ID NO:3.  
     
     
         35 . A vector comprising the nucleic acid molecule of  claim 34 .  
     
     
         36 . A host cell comprising the vector of  claim 35 .  
     
     
         37 . A process for producing a protein comprising a polypeptide comprising amino acid residues 9 to 212 of SEQ ID NO:3, said process comprising culturing the host cell of  claim 36  under conditions suitable for expression of said polypeptide and recovering said protein.