Method of separating protective components of bordetella pertussis

Abstract

To provide a method of efficiently separate protective components of Bordetella pertussis. On the basis of differences in adsorbability to calcium phosphate gel formed by adding calcium ions to a Bordetella pertussis culture in the presence of excess phosphate ions, protective components of Bordetella pertussis are separated from the Bordetella pertussis culture. Traditionally, protective components of Bordetella pertussis have been separated using different purification methods for the respective components. According to the present invention, the use of the same means of purification for all subject components makes it possible to purify each component with high efficiency and high recovery rate, an aspect very advantageous for industrial production. It is also possible to efficiently produce an improved purified pertussis component vaccine comprising an effective combination of pertussis filamentous hemagglutinin (FHA), pertactin (PRN, 69K-OMP), pertussis fimbriae (FIM) and pertussis toxin (PT).

1 - 15 . (canceled)  
     
     
         16 . A pertussis vaccine comprising the protective components of  Bordetella pertussis  pertussis toxin (PT) and pertussis filamentous hemagglutinin (FHA), wherein said components are admixed in a weight ratio of 1:1 μg protein, and said vaccine further comprises a protective component pertactin (PRN) of  Bordetella pertussis.    
     
     
         17 . The pertussis vaccine according to  claim 16 , wherein said components are admixed in a weight ratio of about 1:1:0.4 μg protein of PT:FHA:PRN.  
     
     
         18 . The pertussis vaccine according to  claim 16 , further comprising the protective component pertussis fimbiaie (FIM) of  Bordetella pertussis.    
     
     
         19 . The pertussis vaccine according to  claim 18 , wherein said components are admixed in a weight ratio of about 1:1:0.2-0.4:0.2-0.4 μg protein of PT:FHA:PRN:FIM.  
     
     
         20 . The pertussis vaccine according to  claim 19 , wherein said components are admixed in a weight ratio of about 1:1:0.4:0.2 μg protein of PT:FHA:PRN:FIM.  
     
     
         21 . A pertussis vaccine comprising the protective components of  Bordetella pertussis  PT and FHA, wherein said components are admixed in a weight ratio of 1:1 μg protein, and said vaccine further comprises a protective component FIM of  Bordetella pertussis.    
     
     
         22 . The pertussis vaccine according to  claim 21 , wherein said components are admixed in a weight ratio of about 1:1:0.2 μg protein of PT:FHA:FIM.  
     
     
         23 . The pertussis vaccine according to  claim 16 , wherein said components are admixed in a weight ratio of 1:1:0.2-0.4 μg protein of PT:FHA:PRN.  
     
     
         24 . The pertussis vaccine according to  claim 23 , wherein said components are admixed in a weight ratio of 1:1:0.4 μg protein of PT:FHA:PRN.  
     
     
         25 . The pertussis vaccine according to  claim 18 , wherein said components are admixed in a weight ratio of 1:1:0.2-0.4:0.2-0.4 μg protein of PT:FHA:PRN:FIM.  
     
     
         26 . The pertussis vaccine according to  claim 25 , wherein said components are admixed in a weight ratio of 1:1:0.4:0.2 μg protein of PT:FHA:PRN:FIM.  
     
     
         27 . The pertussis vaccine according to  claim 21 , wherein said components are admixed in a weight ratio of 1:1:0.2-0.4 μg protein of PT:FHA:FIM.  
     
     
         28 . The pertussis vaccine according to  claim 27 , wherein said components are admixed in a weight ratio of 1:1:0.2 μg protein of PT:FHA:FIM.