Single-phase amplification of nucleic acids

Abstract

The amplification of nucleic acids in a single phase can reliably provide products at relatively low cost and labor. A single-phase amplification can also increase the amount of nucleic acids while preserving the relative abundance of the individual nucleic acid species, or portions thereof. A single-phase amplified nucleic acid preparation may be analyzed in a gene expression monitoring system, preferably involving a nucleic acid probe array.

1 - 13 . (canceled)  
     
     
         14 . An amplified nucleic acid preparation comprising RNA obtained by the method for amplification of nucleic acid comprising: 
 synthesizing double-stranded DNA from a single-stranded DNA population, and producing multiple copies of RNA from said double-stranded DNA, wherein said amplification occurs in a single phase.    
     
     
         15 . The Amplified nucleic acid preparation comprising RNA of  claim 14;  wherein said amplification is proportional.  
     
     
         16 - 24 . (canceled)