Degradation of Cercosporin by laccase

Abstract

Disease in plants caused by infection with cercosporin producing strains of Cercospora may be controlled by application of laccase. When applied to the locus of the plant, or parts or seed thereof, the cercosporin toxin produced by this pathogen is degraded by the enzyme, thereby preventing or minimizing damage to the plant. The laccase may be applied alone, or in combination with one or more laccase-producing microorganisms.

1 . A method for controlling disease in plants caused by infection with cercosporin producing species of  Cercospora  comprising applying a cercosporin-degrading effective amount of a composition comprising laccase to a plant or parts thereof, wherein said composition comprises a substantially cell-free extract recovered from a laccase producing organism.  
     
     
         2 . The method of  claim 1  wherein said composition comprises substantially pure laccase.  
     
     
         3 . The method of  claim 1  wherein said composition comprises a cell-free extract recovered from a laccase producing organism.  
     
     
         4 . The method of  claim 3  wherein said laccase producing organism is a first microorganism selected from the group consisting of bacteria and fungi, and said composition comprises a culture medium produced by a culture of said first microorganism and which is free of cells of said first microorganism.  
     
     
         5 . The method of  claim 1  wherein said plant is selected from the group consisting of sugar beets, carrots, soybeans, Juniper trees, Cedar trees, turfgrasses, alfalfa, watermelon, pearl millet, papaya, maize, celery, prunes, and rhododendrons.  
     
     
         6 . The method of  claim 5  wherein said plant is a sugar beet.  
     
     
         7 . The method of  claim 1  wherein said composition is applied onto the foliage of said plant.  
     
     
         8 . The method of  claim 1  wherein said composition is applied onto the harvested root of said plant.  
     
     
         9 . The method of  claim 8  wherein said root of said plant is selected from the group consisting of sugar beets and carrots.  
     
     
         10 . The method of  claim 1  further comprising applying a cercosporin-degrading effective amount of a laccase-producing microorganism to the locus of said plant or seed of said plant.  
     
     
         11 . The method of  claim 10  wherein said laccase-producing microorganism is applied onto the soil in the vicinity of said plant or seed thereof.  
     
     
         12 . (canceled)  
     
     
         13 . (canceled)  
     
     
         14 . The method of  claim 1  wherein laccase is applied to a field crop in an amount greater than or equal to about 5,000 Units of laccase per acre.  
     
     
         15 . The method of  claim 14  wherein laccase is applied to a field crop in an amount greater than or equal to about 5,000 Units of laccase per acre, and less than or equal to about 20,000 Units of laccase per acre.  
     
     
         16 . A method for controlling disease in plants from infection with cercosporin producing strains of  Cercospora  comprising applying a composition which comprises laccase in an amount effective to degrade cercosporin, to the locus of said plant or parts or seed thereof, wherein said composition is selected from the group consisting of substantially pure laccase, a substantially cell-free extract recovered from a laccase producing organism, and a culture medium produced by a culture of a first laccase-producing microorganism and which is substantially free of cells of said first microorganism.  
     
     
         17 . The method of  claim 16  wherein said composition comprises said substantially pure laccase.  
     
     
         18 . The method of  claim 16  wherein said composition comprises said substantially cell-free extract recovered from a laccase producing organism.  
     
     
         19 . The method of  claim 16  wherein said composition comprises said culture medium produced by a culture of a first laccase-producing microorganism and which is substantially free of cells of said first microorganism.  
     
     
         20 . The method of  claim 16  wherein said composition is applied onto the soil in the vicinity of said plant or seed thereof.  
     
     
         21 . The method of  claim 16  wherein said plant is selected from the group consisting of sugar beets, carrots, soybeans, Juniper trees, Cedar trees, turfgrasses, alfalfa, watermelon, pearl millet, papaya, maize, celery, prunes, and rhododendrons.  
     
     
         22 . The method of  claim 21  wherein said plant is a sugar beet.  
     
     
         23 . The method of  claim 18  wherein said extract recovered from a laccase producing organism is cell-free.  
     
     
         24 . The method of  claim 19  wherein said culture medium produced by a culture of said first laccase-producing microorganism is free of cells of said first microorganism.