Nucleic acids encoding human inositolpolyphosphate 5-phosphatase

Abstract

Reagents which regulate human inositol polyphosphate 5-phosphatase and reagents which bind to human inositol polyphosphate 5-phosphatase gene products can play a role in preventing, ameliorating, or correcting dysfunctions or diseases including, but not limited to, COPD, asthma, diabetes, and cancer.

1 - 71 . (canceled)  
     
     
         72 . An isolated and purified polynucleotide which encodes an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOS:2 and 12.  
     
     
         73 . The polynucleotide of  claim 72  which comprises a nucleotide sequence selected from the group consisting of the nucleotide sequences shown in SEQ ID NOS:1 and 11.  
     
     
         74 . The polynucleotide of  claim 72  which is a cDNA.  
     
     
         75 . An isolated and purified single-stranded polynucleotide comprising at least 1950 contiguous nucleotides of a coding sequence or a complement of the coding sequence for an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOS:2 and 12.  
     
     
         76 . The polynucleotide of  claim 75  wherein the protein comprises an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOS:2 and 12 and a nucleotide sequence selected from the group consisting of the nucleotide sequences shown in SEQ ID NOS: 1 and 11.  
     
     
         77 . An expression construct, comprising; 
 a coding sequence for an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOS:2 and 12; and    a promoter which is located upstream from the coding sequence and which controls expression of the coding sequence.    
     
     
         78 . The expression construct of  claim 77  wherein the coding sequence comprises a nucleotide sequence selected from the group consisting of the nucleotide sequences shown in SEQ ID NOS:1 and 11.  
     
     
         79 . A host cell comprising an expression construct, wherein the expression construct comprises: 
 a coding sequence for a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOS:2 and 12; and    a promoter which is located upstream from the coding sequence and which controls expression of the coding sequence.    
     
     
         80 . The host cell of  claim 79  which is prokaryotic.  
     
     
         81 . The host cell of  claim 79  which is eukaryotic.  
     
     
         82 . A method of producing a protein, comprising the steps of: 
 culturing a host cell in a culture medium, wherein the host cell comprises an expression construct comprising (a) a coding sequence for a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOS:2 and 12 and (b) a promoter which is located upstream from the coding sequence and which controls expression of the coding sequence, wherein the step of culturing is carried out under conditions whereby the protein is expressed; and    recovering the protein.