US2005169853A1PendingUtilityA1

Synthetic peptides containing protective epitopes for the treatment and prevention of periodontitis associated with Porphyromonas gingivalis

Assignee: VICTORIAN DAIRY INDUSTRY ASSPriority: Mar 1, 1999Filed: Mar 22, 2005Published: Aug 4, 2005
Est. expiryMar 1, 2019(expired)· nominal 20-yr term from priority
A61P 37/00A61P 1/02A61Q 11/00A61K 2039/6037A61K 8/64A61K 39/0216C07K 16/40
46
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Claims

Abstract

This invention relates to a peptide selected from the group: FLLDADHNTFGSVIPATGPLFTGTASS LYSANFESLIPANADPVVT-TQNIIVTG LYSANFEYLIPANADPVVTTQNIJVTG TNPEPASGKMWIAGDGGNQP RYDDFTFEAGKKYTFTMRRAGMGDGTD DDYVFEAGKKYHFLLLMKKMGSGDGTE TNPEPASGKMWIAGDGGNQPARYDDFTFEAGKKYTFTMRRAGMGDGTD NTFGSVIPATGPL PASGKMWIAGDG EAGKKYTFTMRRA EAGKKYHFLMKKM. It also relates to compositions and use of these peptides for treating and testing Porphyromonas gingialis.

Claims

exact text as granted — not AI-modified
1 . A composition for use in raising an immune response against  Porphyromonas gingivalis , the composition comprising a suitable adjuvant and/or acceptable carrier or excipient and at least one peptide of not more than 50 amino acids which peptide includes at least one  P. gingivalis  epitope, or multimers of said peptide, the at least one  P. gingivalis  epitope being selected from the epitopes included within a peptide selected from the group consisting of:  
       
         
           
                 
                 
               
                     
                 
                   (SEQ ID NO:1) 
                     
                 
                 
                 
               
                   FLLDADHNTFGSVIPATGPLFTGTASS, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:2) 
                     
                 
                 
                 
               
                   LYSANFESLIPANADPVVTTQNIIVTG, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:3) 
                     
                 
                 
                 
               
                   LYSANFEYLIPANADPVVTTQNIIVTG, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:4) 
                     
                 
                 
                 
               
                   TNPEPASGKMWIAGDGGNQP 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:5) 
                     
                 
                 
                 
               
                   RYDDFTFEAGKKYTFTMRRAGMGDGTD, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:6) 
                     
                 
                 
                 
               
                   DDYVFEAGKKYHFLMKKMGSGDGTE 
                     
                 
                   and 
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:7) 
                     
                 
                 
                 
               
                   TNPEPASGKMWLAGDGGNQPARYDDFTFEAGKKYTFTMRRAGMGDGTD. 
                     
                 
                     
                 
             
                
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
                
               
            
             
                
               
            
             
                
                
               
            
           
         
       
     
     
         2 . A composition as claimed in  claim 1  in which the at least one peptide comprises a sequence selected from the group consisting of:  
       
         
           
                 
                 
                 
                 
               
                     
                     
                 
                     
                   NTFGSVIPATGPL, 
                   (SEQ ID NO:8) 
                     
                 
                     
                     
                 
                     
                   LYSANFESLIPANADPVVTTQNIIVTG, 
                   (SEQ ID NO:2) 
                 
                     
                     
                 
                     
                   LYSANFEYLIPANADPVVTTQNIIVTG, 
                   (SEQ ID NO:3) 
                 
                     
                     
                 
                     
                   PASGKMWLAGDG, 
                   (SEQ ID NO:9) 
                 
                     
                     
                 
                     
                   EAGKKYTFTMRRA 
                   (SEQ ID NO:10) 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   EAGKKYHFLMKKM. 
                   (SEQ ID NO:11) 
                 
                     
                     
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         3 . A composition as claimed in  claim 1  in which the at least one peptide comprises a sequence selected from the group consisting of:  
       
         
           
                 
                 
               
                     
                 
                   (SEQ ID NO:1) 
                     
                 
                 
                 
               
                   FLLDADHNTFGSVIPATGPLFTGTASS, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:2) 
                     
                 
                 
                 
               
                   LYSANFESLIPANADPVVTTQNIIVTG, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:3) 
                     
                 
                 
                 
               
                   LYSANFEYLIPANADPVVTTQNIIVTG, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:4) 
                     
                 
                 
                 
               
                   TNPEPASGKMWIAGDGGNQP, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:5) 
                     
                 
                 
                 
               
                   RYDDFTFEAGKKYTFTMRRAGMGDGTD, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:6) 
                     
                 
                 
                 
               
                   DDYVFEAGKKYHFLMKKMGSGDGTE 
                     
                 
                   and 
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:7) 
                     
                 
                 
                 
               
                   TNPEPASGKMWIAGDGGNQPARYDDFTFEAGKKYTFTMRRAGMGDGTD. 
                     
                 
                     
                 
             
                
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
                
               
            
             
                
               
            
             
                
                
               
            
           
         
       
     
     
         4 . A composition as claimed in  claim 1  in which the at least one peptide is selected from the group consisting of:  
       
         
           
                 
                 
               
                     
                 
                   (SEQ ID NO:1) 
                     
                 
                 
                 
               
                   FLLDADHNTFGSVIPATGPLFTGTASS, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:2) 
                     
                 
                 
                 
               
                   LYSANFESLIPANADPVVTTQNIIVTG, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:3) 
                     
                 
                 
                 
               
                   LYSANFEYLIPANADPVVTTQNIIVTG, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:4) 
                     
                 
                 
                 
               
                   TNPEPASGKMWIAGDGGNQP, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:5) 
                     
                 
                 
                 
               
                   RYDDFTFEAGKKYTFTMRRAGMGDGTD, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:6) 
                     
                 
                 
                 
               
                   DDYVFEAGKKYHFLMKKMGSGDGTE 
                     
                 
                   and 
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:7) 
                     
                 
                 
                 
               
                   TNPEPASGKMWIAGDGGNQPARYDDFTFEAGKKYTFTMRRAGMGDGTD. 
                     
                 
                     
                 
             
                
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
                
               
            
             
                
               
            
             
                
                
               
            
           
         
       
     
     
         5 . A composition as claimed in  claim 1  which comprises more than one peptide.  
     
     
         6 . A composition as claimed in  claim 5  in which the peptides are in multimeric form(s).  
     
     
         7 . A composition as claimed in  claim 6  in which the multimer(s) comprise different peptides.  
     
     
         8 . A peptide having no more than 50 amino acids which peptide comprises at least one  P. gingivalis  epitope, the at least one  P. gingivalis  epitope being selected from the epitopes included within a peptide selected from the group consisting of:  
       
         
           
                 
                 
               
                     
                 
                   (SEQ ID NO:1) 
                     
                 
                 
                 
               
                   FLLDADHNTFGSVIPATGPLFTGTASS, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:2) 
                     
                 
                 
                 
               
                   LYSANFESLIPANADPVVTTQNIIVTG, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:3) 
                     
                 
                 
                 
               
                   LYSANFEYLIPANADPVVTTQNIIVTG, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:4) 
                     
                 
                 
                 
               
                   TNPEPASGKMWLAGDGGNQP, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:5) 
                     
                 
                 
                 
               
                   RYDDFTFEAGKKYTFTMRRAGMGDGTD, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:6) 
                     
                 
                 
                 
               
                   DDYVFEAGKKYHFLMKKMGSGDGTE 
                     
                 
                   and 
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:7) 
                     
                 
                 
                 
               
                   TNPEPASGKMWIAGDGGNQPARYDDFTFEAGKKYTFTMRRAGMGDGTD. 
                     
                 
                     
                 
             
                
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
                
               
            
             
                
               
            
             
                
                
               
            
           
         
       
     
     
         9 . A peptide as claimed in  claim 8  comprising at least one sequence selected from the group consisting of:  
       
         
           
                 
                 
                 
                 
               
                     
                     
                 
                     
                   NTFGSVIPATGPL, 
                   (SEQ ID NO:8) 
                     
                 
                     
                     
                 
                     
                   LYSANFESLIPANADPVVTTQNIIVTG, 
                   (SEQ ID NO:2) 
                 
                     
                     
                 
                     
                   LYSANFEYLIPANADPVVTTQNIIVTG, 
                   (SEQ ID NO:3) 
                 
                     
                     
                 
                     
                   PASGKMWLAGDG, 
                   (SEQ ID NO:9), 
                 
                     
                     
                 
                     
                   EAGKKYTFTFMRRA 
                   (SEQ ID NO:10) 
                 
                     
                   and 
                 
                     
                     
                 
                     
                   EAGKKYHFLMKKM. 
                   (SEQ ID NO:11) 
                 
                     
                     
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         10 . A peptide as claimed in  claim 8  comprising at least one sequence selected from the group consisting of:  
       
         
           
                 
                 
               
                     
                 
                   (SEQ ID NO:1) 
                     
                 
                 
                 
               
                   FLLDADHNTFGSVIPATGPLFTGTASS, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:2) 
                     
                 
                 
                 
               
                   LYSANFESLIPANADPVVTTQNIIVTG, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:3) 
                     
                 
                 
                 
               
                   LYSANFEYLIPANADPVVTTQNIIVTG, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:4) 
                     
                 
                 
                 
               
                   TNPEPASGKMWIAGDGGNQP, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:5) 
                     
                 
                 
                 
               
                   RYDDFTFEAGKKYTFTMRRAGMGDGTD, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:6) 
                     
                 
                 
                 
               
                   DDYVFEAGKKYHFLMKKMGSGDGTE 
                     
                 
                   and 
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:7) 
                     
                 
                 
                 
               
                   TNPEPASGKMWIAGDGGNQPARYDDFTFEAGKKYTFTMRRAGMGDGTD. 
                     
                 
                     
                 
             
                
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
                
               
            
             
                
               
            
             
                
                
               
            
           
         
       
     
     
         11 . A peptide as claimed in  claim 8  in which the peptide is selected from the group consisting of:  
       
         
           
                 
                 
               
                     
                 
                   (SEQ ID NO:1) 
                     
                 
                 
                 
               
                   FLLDADHNTFGSVIPATGPLFTGTASS, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:2) 
                     
                 
                 
                 
               
                   LYSANFESLIPANADPVVTTQNIIVTG, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:3) 
                     
                 
                 
                 
               
                   LYSANFEYLIPANADPVVTTQNIIVTG, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:4) 
                     
                 
                 
                 
               
                   TNPEPASGKMWIAGDGGNQP, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:5) 
                     
                 
                 
                 
               
                   RYDDFTFEAGKKYTFTMRRAGMGDGTD, 
                     
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:6) 
                     
                 
                 
                 
               
                   DYVFEAGKKYHFLMKKMGSGDGTE 
                     
                 
                   and 
                 
                     
                 
                 
                 
               
                   (SEQ ID NO:7) 
                     
                 
                 
                 
               
                   TNPEPASGKMWIAGDGGNQPARYDDFTFEAGKKYTFTMRRAGMGDGTD. 
                     
                 
                     
                 
             
                
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
               
            
             
                
               
            
             
                
                
                
               
            
             
                
               
            
             
                
                
               
            
           
         
       
     
     
         12 . Use of a peptide as claimed in  claim 8  as an antigen in a diagnostic test.  
     
     
         13 . An antibody specifically directed against a composition as clamed in  claim 1  or a peptide as defined above.  
     
     
         14 . An antibody as claimed in  claim 13  which is a monoclonal antibody.  
     
     
         15 . Use of an antibody as claimed in  claim 13  in a diagnostic test.  
     
     
         16 . A composition comprising an antibody as claimed in  claim 13  and a pharmaceutically acceptable carrier or diluent.  
     
     
         17 . A method of reducing the prospect of  P. gingivalis  infection in an individual and/or severity of disease, the method comprising administering to the individual an amount of a composition as claimed in  claim 1  effective to induce an immune response in the individual directed against  P. Gingivalis.    
     
     
         18 . A method of reducing the prospect of  P. gingivalis  infection in an individual and/or severity of disease, the method comprising administering to the individual an effective amount of a composition as claimed in  16 .

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