Development of DNA probes and immunological reagents specific for cell surface-expressed molecules and transformation-associated genes

Abstract

This invention provides a method for preparing a hybridoma cell line which produces an antibody capable of specifically binding to a cell surface-expressed protein which expresses on the surface of one cell type but not the other. This invention also provides a method for preparing a hybridoma cell line which produces an antibody capable of specifically binding to a cell surface-expressed protein. This invention provides a method to prepare a hybridoma cell line which specifically recognizes and binds to a tumor associated antigen associated with a neoplastic, human cell. This invention also provides a method of preparing DNA encoding a cell surface antigen associated with a neoplastic, human cell. This invention further provides an isolated mammalian nucleic acid molecule having the sequence of Prostate Carcinoma Tumor Antigen Gene-1. This invention also provides an isolated mammalian nucleic acid molecule having the sequence of Prostate Tumor Inducing Gene-1. This invention provides an isolated mammalian nucleic acid molecule having the sequence of Prostate Tumor Inducing Gene-2. Finally, this invention provides an isolated mammalian nucleic acid molecule having the sequence of Prostate Tumor Inducing Gene-1.

1 - 127 . (canceled)  
     
     
         128 . A purified mammalian Prostate Tumor Inducing Gene-1 protein.  
     
     
         129 . The purified mammalian Prostate Tumor Inducing Gene-1 protein of  claim 128 , wherein the protein comprises the amino acid sequence of SEQ ID NO:17.  
     
     
         130 . An antibody capable of specifically binding to the mammalian Prostate Tumor Inducing Gene-1 protein of  claim 128 .  
     
     
         131 . The antibody of  claim 130 , wherein the antibody is a monoclonal antibody.  
     
     
         132 . A composition comprising an antibody of  claim 130  and a pharmaceutically acceptable carrier.  
     
     
         133 . The antibody of  claim 130  coupled to a cytotoxic agent.  
     
     
         134 . The antibody of  claim 133 , wherein the cytotoxic agent is a radioisotope or a toxin.  
     
     
         135 . An immunoassay for detecting the presence of a mammalian Prostate Tumor Inducing Gene-1 protein in a biological sample comprising the steps of: 
 a) contacting the biological sample with the antibody of  claim 130  under conditions permitting the formation of a complex between said antibody and the mammalian Prostate Tumor Inducing Gene-1 protein; and    b) detecting said complex, wherein detection of said complex indicates the presence of mammalian Prostate Tumor Inducing Gene-1 protein in a biological sample.    
     
     
         136 . A mammalian Prostate Tumor Inducing Gene-1 protein bound to the antibody of  claim 130 .  
     
     
         137 . A method for detecting cancer cells in a sample comprising detection of the expression of Prostate Tumor Inducing Gene-1 in the sample, comprising the steps of: 
 a) contacting the sample with an antibody capable of specifically recognizing PTI-1 protein under conditions permitting the formation of a complex between the PTI-1 protein and the antibody; and    b) measuring the complex formed,    thereby detecting cancer cells in the sample.    
     
     
         138 . The method of  claim 137 , wherein the antibody is a monoclonal antibody.