Mammalian CDP-diacylglycerol synthase

Abstract

There is disclosed cDNA sequences and polypeptides having the enzyme CDP-diacylglycerol synthase (CDS) activity. CDS is also known as CTP:phosphatidate cytidylyltransferase. There is further disclosed methods for isolation and production of polypeptides involved in phosphatidic acid metabolism and signaling in mammalian cells, in particular, the production of purified forms of CDS.

1 - 16 . (canceled)  
     
     
         17 . A method for determining whether a compound is a useful pharmaceutical compound, comprising (a) contacting a first sample of a polypeptide that comprises an amino acid sequence of  FIG. 8  (SEQ ID NO: 12) that has CDP-diacylglycerol synthase (CDS) activity with (i) at least one substrate for the polypeptide and (ii) at least one compound; (b) contacting a second sample of the polypeptide with the substrate but not with the compound; and (c) determining whether the CDS activity of the polypeptide of the first sample is increased or decreased in comparison to the CDS activity of the polypeptide of the second sample, wherein an increase or decrease in the CDS activity of the polypeptide of the first sample indicates that the compound modulates CDS activity and is a useful pharmaceutical compound.