Magnetic fine particles having lower critical solution temperature

Abstract

The present invention relates to magnetic fine particles having a lower critical solution temperature to which at least one substance selected from biotin and avidin is immobilized, and a method of converting a substance, a method of separating or concentrating a microorganism, a method of modifying a denatured protein, a method of detecting a nucleic acid, a separating agent, and a method of separating a biological substance using the same.

1 . (canceled)  
     
     
         2 . The method according to  claim 9 , wherein said at least one substance selected from biotin and avidin is immobilized to the magnetic fine particles through a polymer having a lower critical solution temperature.  
     
     
         3 . The method according to  claim 9 , wherein said at least one substance is biotin.  
     
     
         4 . The method according to  claim 3 , wherein said biotin is immobilized to the magnetic fine particles through a polymer having a lower critical solution temperature.  
     
     
         5 . The method according to  claim 3 , wherein said biotin is biotin bound to avidin (hereinafter referred to as “avidin-bound biotin”).  
     
     
         6 . The method according to  claim 9 , wherein a substance having a mutual specific action with an objective substance and having immobilized thereto avidin is bound to said biotin.  
     
     
         7 . The method according to  claim 9 , wherein a substance having a mutual specific action with an objective substance and having immobilized thereto biotin is bound to said avidin.  
     
     
         8 . The method according to  claim 6 , wherein said substance which has a mutual specific action with an objective substance is at least one substance selected from enzymes, proteins, nucleic acids, peptides, molecular chaperon, heat shock proteins, and antibodies.  
     
     
         9 . A conversion method comprising converting a substance using magnetic fine particles having a lower critical solution temperature, and having immobilized thereto at least one substance selected from biotin and avidin.  
     
     
         10 - 16 . (canceled)  
     
     
         17 . The method according to  claim 4 , wherein said biotin is biotin bound to avidin (hereinafter referred to as “avidin-bound biotin”).  
     
     
         18 . The method according to  claim 2 , wherein a substance having a mutual specific action with an objective substance and having immobilized thereto avidin is bound to said biotin.  
     
     
         19 . The method according to  claim 3 , wherein a substance having a mutual specific action with an objective substance and having immobilized thereto avidin is bound to said biotin.  
     
     
         20 . The method according to  claim 4 , wherein a substance having a mutual specific action with an objective substance and having immobilized thereto avidin is bound to said biotin.  
     
     
         21 . The method according to  claim 2 , wherein a substance having a mutual specific action with an objective substance and having immobilized thereto biotin is bound to said avidin.  
     
     
         22 . The method according to  claim 5 , wherein a substance having a mutual specific action with an objective substance and having immobilized thereto biotin is bound to said avidin.  
     
     
         23 . The method according to  claim 7 , wherein said substance which has a mutual specific action with an objective substance is at least one substance selected from enzymes, proteins, nucleic acids, peptides, molecular chaperon, heat shock proteins, and antibodies.