Seed coat specific DNA regulatory region and peroxidase

Abstract

A novel seed coat specific peroxidase genomic sequence is characterized and presented. The seed coat peroxidase is translated as a 352 amino acid precursor protein of 38 kDa comprising a 26 amino acid signal sequence which when cleaved results in a 35 kDa protein. Probes derived from the cDNA, or genomic DNA can be used to detect polymorphisms that distinguish EpEp and epep genotypes. The regulatory region of the seed coat specific gene may be used to control expression of genes of interest such as genes encoding herbicide resistance, biological control of insects or pathogens, viral coat proteins to protect against viral infections, proteins of commercial interest, or proteins that alter the nutritive value, taste, or processing of seeds.

1 . (canceled)  
     
     
         2 . An isolated DNA molecule comprising at least 24 contiguous nucleotides selected from nucleotides 1-1532 of SEQ ID NO:2.  
     
     
         3 - 29 . (canceled)  
     
     
         30 . A chimeric DNA molecule, said DNA molecule comprising a segment of a plant expressible promoter, providing a TATA box region and a transcription start site, whereby a heterologous plant expressible structural gene, placed 3′ to said segment of the plant expressible promoter is expressed under the regulatory control of said segment of the plant expressible promoter, and wherein said segment of the plant expressible promoter is selected from the group consisting of: 
 a) a nucleotide sequence consisting of nucleotides 9 to 1532 of SEQ ID NO:2;    b) a nucleotide sequence consisting of nucleotides 459 to 1532 of SEQ ID NO:2;    c) a nucleotide sequence consisting of nucleotides 1066 to 1532 of SEQ ID NO:2; and    d) a nucleotide sequence consisting of nucleotides 1326 to 1532 of SEQ ID NO:2.