Biomarkers for liver diseases and method for using the same

Abstract

Biomarkers for liver diseases and method for using the same are provided. For detecting liver cirrhosis and liver cancer, the biomarkers are selected from any one of the amino acid sequences with SEQ ID NO:1 to SEQ ID NO:24 or derivatives or fragments or variants or the combination thereof or the antibodies against the amino acid sequences. Then the biomarkers are further developed into detection kits, such that by detecting the existence of autoantibodies or autoantigens in screened specimens, liver diseases are detected with higher accuracy and sensitivity.

1 . A Biomarker for liver diseases selected from any one of the amino acid sequences with SEQ ID NO:1 to SEQ ID NO:24 or derivatives or fragments or variants or the combination thereof or the antibodies against said amino acid sequences.  
     
     
         2 . The biomarker according to  claim 1 , wherein said liver disease is liver cirrhosis or liver cancer.  
     
     
         3 . The biomarker according to  claim 1 , wherein said variant and any one of the amino acid sequences with SEQ ID NO:1 to SEQ ID NO:24 show sequence homology greater than 80%.  
     
     
         4 . A detection kit for liver diseases, comprising biomarkers selected from any one of the amino acid sequences with SEQ ID NO:1 to SEQ ID NO:24 or derivatives or fragments or variants or the combination thereof.  
     
     
         5 . The detection kit according to  claim 4 , wherein said liver disease is liver cirrhosis or liver cancer.  
     
     
         6 . The detection kit according to  claim 4 , wherein the detection kit can further include secondary antibodies that can recognize autoantibody against any one of the amino acid sequences with SEQ ID NO:1 to SEQ ID NO:24 or derivatives or fragments or variants or the combination thereof.  
     
     
         7 . A method for screening liver diseases, comprising the steps of: 
 providing a specimen;    using a biomarker selected from any one of the amino acid sequences with SEQ ID NO:1 to SEQ ID NO:24 or derivatives or fragments or variants or the combination thereof to identify and capture autoantibodies in the specimen; and    detecting the autoantibodies.    
     
     
         8 . The method according to  claim 7 , wherein said specimen includes whole blood or serum.  
     
     
         9 . The method according to  claim 8 , wherein said specimen is serum.  
     
     
         10 . The method according to  claim 7 , wherein said biomarker may be made into detection kits.  
     
     
         11 . The method according to  claim 7 , wherein said biomarker is firstly immobilized on a substrate.  
     
     
         12 . The method according to  claim 11 , wherein said substrate is a immunoassay plate or a biochip.  
     
     
         13 . The method according to  claim 7 , wherein said specimen is firstly labeled with a fluorescence marker.  
     
     
         14 . The method according to  claim 7 , wherein the method further include a step of using a secondary antibody to recognize and adsorb the autoantibody.  
     
     
         15 . The method according to  claim 14 , wherein said secondary antibody is modified and has a special functional group detectable by means of color reaction, radioactivity or fluorescence.  
     
     
         16 . The method according to  claim 7 , wherein the detection of the autoantibody is achieved by using a fluorescence scanner to detect a fluorescence-labeled autoantibody.  
     
     
         17 . The method according to  claim 7 , wherein the detection of the autoantibody is achieved by detection of the secondary autoantibody with enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA) or immunofluorescence.  
     
     
         18 . A detection kit for liver diseases, comprising a set of antibodies against any one of amino acid sequences with SEQ ID NO:1 to SEQ ID NO:24.  
     
     
         19 . The detection kit according to  claim 18 , wherein said liver disease is liver cirrhosis or liver cancer.  
     
     
         20 . A screening method for liver diseases, comprising the steps of: 
 providing a specimen;    using an antibody against any one of the amino acid sequences with SEQ ID NO:1 to SEQ ID NO:24 to capture an antigen in the specimen; and    detecting the antibody-antigen complex.    
     
     
         21 . The screening method according to  claim 20 , wherein said specimen is whole blood or serum.  
     
     
         22 . The screening method according to  claim 21 , wherein said specimen is serum.