Insulin-like growth factor binding protein (IGFBP-5)

Abstract

A purified insulin-like growth factor binding protein (IGFBP) selected from the group consisting of insulin-like growth factor binding protein having an amino acid sequence that, preferably, is at least 70% homologous to the amino acid sequence of FIG. 1 and fragments thereof that are capable of binding to an antibody specific for the protein or to an insulin-like growth factor is described. This new IGFBP is designated herein as IGFBP-6. Recombinant DNA molecules encoding the binding proteins and subsequences thereof are also described along with recombinant microorganisms and cell lines containing the DNA molecules and methods for producing the binding proteins using recombinant hosts containing the relevant DNA molecules. Antibodies to the protein, useful in various diagnostic applications, are also described.

1 . A purified binding protein selected from the group consisting of an insulin-like growth factor binding protein (IGFPB) having an amino acid sequence that is at least 70% homologous to the amino acid sequence of  FIG. 1  and fragments thereof comprising at least 10 consecutive amino acids of the sequence that are capable of binding to an antibody specific for the binding protein or to an insulin-like growth factor.  
     
     
         2 . The binding protein of  claim 1 , wherein the binding protein comprises the amino acid sequence of  FIG. 1 .  
     
     
         3 . The binding protein of  claim 1 , wherein the binding protein comprises a fragment.  
     
     
         4 . A composition comprising human protein, wherein the human protein consists of the purified protein of  claim 1 .  
     
     
         5 . Recombinant insulin-like growth factor binding protein 6 (IGFBP-6).  
     
     
         6 . An antibody, antibody fragment, or derivative thereof that recognizes IGFBP-6.  
     
     
         7 . A recombinant DNA molecule comprising a nucleic acid sequence encoding IGFBP-6 or a subsequence thereof comprising at least 10 nucleotides.  
     
     
         8 . The recombinant DNA molecule of  claim 7 , wherein the sequence is as set forth for IGFBP-6 in  FIG. 1 .  
     
     
         9 . The recombinant DNA molecule of  claim 7 , wherein the sequence is a human DNA sequence.  
     
     
         10 . The recombinant DNA molecule of  claim 7 , wherein the sequence is a genomic sequence.  
     
     
         11 . The recombinant DNA molecule of  claim 7 , wherein the sequence is a cDNA sequence.  
     
     
         12 . The recombinant DNA molecule of  claim 7 , wherein the DNA molecule is contained in pBsBP6.1.  
     
     
         13 . A recombinant microorganism or cell line containing the DNA molecule of  claim 7 .  
     
     
         14 . The microorganism of  claim 13 , wherein the microorganism is a yeast.  
     
     
         15 . The cell line of  claim 13 , wherein the cell line is a CHO cell line.  
     
     
         16 . A method for producing IGFBP-6 or a fragment thereof comprising 
 growing a recombinant host containing the DNA molecule of  claim 7  under conditions wherein the IGFBP-6 or fragment thereof is expressed by the host; and    isolating the expressed IGFBP-6 or fragment thereof.    
     
     
         17 . The method of  claim 16 , wherein the host is a microorganism.  
     
     
         18 . The method of  claim 16 , wherein the host is a eucaryotic cell.  
     
     
         19 . The method of  claim 16 , wherein the host is a non-human, transgenic animal.