Diaminofluorescein derivatives

Abstract

A compound represented by the following formula (I): wherein R 1 and R 2 represent amino groups that substitute at adjacent positions on the phenyl ring, provided that either of R 1 and R 2 represents a mono(C 1-6 alkyl)-substituted amino group and the other represents an unsubstituted amino group; and R 3 and R 4 independently represent hydrogen atom or an acyl group, and an agent for measurement of nitrogen monoxide which comprises said compound.

1 . 4-Amino-5-(N-methylamino)-2′,7′-difluoro-3′,6′-dihydroxy-spiro[isobenzofuran-1 (3H),9′-[9H]xanthen]-3-one (6: DAF-FM).  
     
     
         2 . A composition for measurement of nitrogen monoxide, which comprises a compound according to  claim 1 .  
     
     
         3 . A method for measuring nitrogen monoxide which comprises (1) reacting the (DAF-FM) of  claim 1  with nitrogen monoxide, and (2) detecting a compound formed by the reaction in (1).