Nucleic acid affinity columns

Abstract

This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided.

1 . A method of making a nucleic acid pool comprising a plurality of different nucleic acids, said method comprising the steps of: 
 a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 20 oligonucleotides having different predetermined nucleic acid sequences, and wherein: 
 i) each different oligonucleotide is localized in a predetermined region of said surface;  
 ii) the density of said oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2; and  
 iii) said different oligonucleotides have an identical terminal 5′ nucleic acid sequence;  
   b) amplifying said multiplicity of oligonucleotides at least about 10 fold to provide said nucleic acid pool.    
     
     
         2 - 40 . (canceled)